Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 437, Issue 1-2, Pages 303-305Publisher
ELSEVIER
DOI: 10.1016/j.ijpharm.2012.08.002
Keywords
Serum-free media; CHO cells; Monoclonal antibody; Production; Cell growth
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Funding
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/46661/2008, SFRH/BD/46660/2008]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/46660/2008, SFRH/BD/46661/2008] Funding Source: FCT
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The selection of a serum-free medium for a particular process of production using mammalian cells is a critical step for its success. In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell growth and monoclonal antibody (mAb) production of a transfected CHO-K1 cell line. In the conditions assayed, EX-CELL and particularly CDM4CHO are the most recommended media for extended biopharmaceutical processes, on account of inducing superior levels of cell proliferation and mAb production, accentuated by a tendency to improve over time. Furthermore, the less positive results obtained with some media emphasize the importance and the impact of the correct medium selection. (C) 2012 Elsevier B. V. All rights reserved.
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