Engineered biosynthesis of natural products in heterologous hosts

Rapid prototyping of microbial cell factories via genome-scale engineering

(2015) Tong Si et al.   BIOTECHNOLOGY ADVANCES

Distributing a metabolic pathway among a microbial consortium enhances production of natural products

(2015) Kang Zhou et al.   NATURE BIOTECHNOLOGY

Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming ofPenicillium chrysogenum

(2015) Kirsty J. McLean et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted engineering and scale up of lycopene overproduction in Escherichia coli

(2015) Fayin Zhu et al.   PROCESS BIOCHEMISTRY

Total synthesis of a eukaryotic chromosome: Redesigning and SCRaMbLE-ing yeast

(2014) Dejana Jovicevic et al.   BIOESSAYS

Production of lycopene by metabolically-engineered Escherichia coli

(2014) Tao Sun et al.   BIOTECHNOLOGY LETTERS

Integrating Biological Redesign: Where Synthetic Biology Came From and Where It Needs to Go

(2014) Jeffrey C. Way et al.   CELL

Production of bioactive ginsenoside compound K in metabolically engineered yeast

(2014) Xing Yan et al.   CELL RESEARCH

Synthetic microbial consortia: from systematic analysis to construction and applications

(2014) Hao Song et al.   CHEMICAL SOCIETY REVIEWS

Aryl-aldehyde Formation in Fungal Polyketides: Discovery and Characterization of a Distinct Biosynthetic Mechanism

(2014) Meng Wang et al.   CHEMISTRY & BIOLOGY

Designer microbes for biosynthesis

(2014) Maureen B Quin et al.   CURRENT OPINION IN BIOTECHNOLOGY

Recent advances in natural product discovery

(2014) Yunzi Luo et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering the lactococcal mevalonate pathway for increased sesquiterpene production

(2014) Adelene A. Song et al.   FEMS MICROBIOLOGY LETTERS

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

(2014) Jiazhang Lian et al.   METABOLIC ENGINEERING

Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol

(2014) Jiazhang Lian et al.   METABOLIC ENGINEERING

Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways

(2014) Jianming Yang et al.   Microbial Cell Factories

Linalool Exhibits Cytotoxic Effects by Activating Antitumor Immunity

(2014) Mei-Yin Chang et al.   MOLECULES

Natural product and natural product derived drugs in clinical trials

(2014) Mark S. Butler et al.   NATURAL PRODUCT REPORTS

A semi-synthetic organism with an expanded genetic alphabet

(2014) Denis A. Malyshev et al.   NATURE

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Biosynthesis of Taxadiene in Saccharomyces cerevisiae : Selection of Geranylgeranyl Diphosphate Synthase Directed by a Computer-Aided Docking Strategy

(2014) Ming-zhu Ding et al.   PLoS One

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

Rethinking production of Taxol® (paclitaxel) using endophyte biotechnology

(2014) Souvik Kusari et al.   TRENDS IN BIOTECHNOLOGY

Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae

(2014) Elena Fossati et al.   Nature Communications

Producing aglycons of ginsenosides in bakers' yeast

(2014) Zhubo Dai et al.   Scientific Reports

RNAi-Assisted Genome Evolution in Saccharomyces cerevisiae for Complex Phenotype Engineering

(2014) Tong Si et al.   ACS Synthetic Biology

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Improvement of Reticuline Productivity from Dopamine by Using EngineeredEscherichia coli

(2013) Ju-Sung KIM et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Construction of a controllable β-carotene biosynthetic pathway by decentralized assembly strategy inSaccharomyces cerevisiae

(2013) Wenping Xie et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improved heterologous erythromycin A production through expression plasmid re-design

(2013) Ming Jiang et al.   BIOTECHNOLOGY PROGRESS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Getting to the bottom of Taxol biosynthesis by fungi

(2013) Uwe Heinig et al.   FUNGAL DIVERSITY

Strategies for mining fungal natural products

(2013) Philipp Wiemann et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

An Efficient System for Heterologous Expression of Secondary Metabolite Genes in Aspergillus nidulans

(2013) Yi-Ming Chiang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Deoxysugar pathway interchange for erythromycin analogues heterologously produced through Escherichia coli

(2013) Ming Jiang et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli for production of salvianic acid A via an artificial biosynthetic pathway

(2013) Yuan-Feng Yao et al.   METABOLIC ENGINEERING

Engineering central metabolic modules of Escherichia coli for improving β-carotene production

(2013) Jing Zhao et al.   METABOLIC ENGINEERING

Improving carotenoids production in yeast via adaptive laboratory evolution

(2013) Luis H. Reyes et al.   METABOLIC ENGINEERING

Cloning and Characterization of the Glycoside Hydrolases That Remove Xylosyl Groups from 7-β-xylosyl-10-deacetyltaxol and Its Analogues

(2013) Hai-Li Cheng et al.   MOLECULAR & CELLULAR PROTEOMICS

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Engineering dynamic pathway regulation using stress-response promoters

(2013) Robert H Dahl et al.   NATURE BIOTECHNOLOGY

Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols

(2013) José L Avalos et al.   NATURE BIOTECHNOLOGY

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping

(2013) Hsu-Hua Yeh et al.   ORGANIC LETTERS

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts

(2013) J. Guo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and characterization of synthetic fungal-bacterial consortia for direct production of isobutanol from cellulosic biomass

(2013) Jeremy J. Minty et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Causes and Effects of N-Terminal Codon Bias in Bacterial Genes

(2013) D. B. Goodman et al.   SCIENCE

One pathway, many compounds: heterologous expression of a fungal biosynthetic pathway reveals its intrinsic potential for diversity

(2013) Zahida Wasil et al.   Chemical Science

Programming adaptive control to evolve increased metabolite production

(2013) Howard H. Chou et al.   Nature Communications

Discovery of Cryptic Polyketide Metabolites from Dermatophytes Using Heterologous Expression in Aspergillus nidulans

(2013) Wen-Bing Yin et al.   ACS Synthetic Biology

Multiplex Iterative Plasmid Engineering for Combinatorial Optimization of Metabolic Pathways and Diversification of Protein Coding Sequences

(2013) Yifan Li et al.   ACS Synthetic Biology

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

Yeast Oligo-Mediated Genome Engineering (YOGE)

(2013) James E. DiCarlo et al.   ACS Synthetic Biology

Assembly of eukaryotic algal chromosomes in yeast

(2013) Bogumil J Karas et al.   Journal of Biological Engineering

Controlling promoter strength and regulation inSaccharomyces cerevisiaeusing synthetic hybrid promoters

(2012) John Blazeck et al.   BIOTECHNOLOGY AND BIOENGINEERING

Increase in the production of β-carotene in recombinant Escherichia coli cultured in a chemically defined medium supplemented with amino acids

(2012) Hyun-Koo Nam et al.   BIOTECHNOLOGY LETTERS

Dynamics and Memory of Heterochromatin in Living Cells

(2012) Nathaniel A. Hathaway et al.   CELL

Establishing a New Methodology for Genome Mining and Biosynthesis of Polyketides and Peptides through Yeast Molecular Genetics

(2012) Kan'ichiro Ishiuchi et al.   CHEMBIOCHEM

Functional expression of a foreign gene in Aspergillus oryzae producing new pyrone compounds

(2012) Juntira Punya et al.   FUNGAL GENETICS AND BIOLOGY

Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis

(2012) Adelene Ai Lian Song et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Engineering fungal secondary metabolism: A roadmap to novel compounds

(2012) Daniel H. Scharf et al.   JOURNAL OF BIOTECHNOLOGY

Natural Products As Sources of New Drugs over the 30 Years from 1981 to 2010

(2012) David J. Newman et al.   JOURNAL OF NATURAL PRODUCTS

Modular Pathway Engineering of Diterpenoid Synthases and the Mevalonic Acid Pathway for Miltiradiene Production

(2012) Yongjin J. Zhou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism

(2012) Yun Chen et al.   METABOLIC ENGINEERING

Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production

(2012) Bilge Özaydın et al.   METABOLIC ENGINEERING

Positive genetic interactors of HMG2 identify a new set of genetic perturbations for improving sesquiterpene production in Saccharomyces cerevisiae

(2012) Codruta Ignea et al.   Microbial Cell Factories

Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae

(2012) Gionata Scalcinati et al.   Microbial Cell Factories

New Insights into Plant Isoprenoid Metabolism

(2012) Pablo Pulido et al.   Molecular Plant

Advances in Aspergillus secondary metabolite research in the post-genomic era

(2012) James F. Sanchez et al.   NATURAL PRODUCT REPORTS

Spatiotemporal control of gene expression by a light-switchable transgene system

(2012) Xue Wang et al.   NATURE METHODS

Coordinated induction of multi-gene pathways in Saccharomyces cerevisiae

(2012) Jing Liang et al.   NUCLEIC ACIDS RESEARCH

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

(2012) Rafael Silva-Rocha et al.   NUCLEIC ACIDS RESEARCH

Molecular characterization of the pentacyclic triterpenoid biosynthetic pathway in Catharanthus roseus

(2012) Lili Huang et al.   PLANTA

Overexpressing 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGR) in the Lactococcal Mevalonate Pathway for Heterologous Plant Sesquiterpene Production

(2012) Adelene Ai-Lian Song et al.   PLoS One

Fine-tuning gene networks using simple sequence repeats

(2012) R. G. Egbert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin

(2012) P. J. Westfall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Increase of lycopene production by supplementing auxiliary carbon sources in metabolically engineered Escherichia coli

(2011) Yeong-Su Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Analysis of heterologous taxadiene production in K- and B-derived Escherichia coli

(2011) Brett A. Boghigian et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improved E. coli erythromycin a production through the application of metabolic and bioprocess engineering

(2011) Haoran Zhang et al.   BIOTECHNOLOGY PROGRESS

Isolation and characterization of Stemphylium sedicola SBU-16 as a new endophytic taxol-producing fungus from Taxus baccata grown in Iran

(2011) Mohammad Hossein Mirjalili et al.   FEMS MICROBIOLOGY LETTERS

Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools

(2011) Michael S. Siddiqui et al.   FEMS YEAST RESEARCH

Harnessing yeast subcellular compartments for the production of plant terpenoids

(2011) Moran Farhi et al.   METABOLIC ENGINEERING

Stepwise increase of resveratrol biosynthesis in yeast Saccharomyces cerevisiae by metabolic engineering

(2011) Yechun Wang et al.   METABOLIC ENGINEERING

Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin

(2011) Karin Lemuth et al.   Microbial Cell Factories

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

(2011) Zengyi Shao et al.   Molecular BioSystems

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

(2011) Jessica S. Dymond et al.   NATURE

Elucidation of the Pathway to Astaxanthin in the Flowers ofAdonis aestivalis

(2011) Francis X. Cunningham et al.   PLANT CELL

Organization of Intracellular Reactions with Rationally Designed RNA Assemblies

(2011) C. J. Delebecque et al.   SCIENCE

A Synthetic Optogenetic Transcription Device Enhances Blood-Glucose Homeostasis in Mice

(2011) H. Ye et al.   SCIENCE

A bacterial platform for fermentative production of plant alkaloids

(2011) Akira Nakagawa et al.   Nature Communications

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

Diversion of Flux toward Sesquiterpene Production inSaccharomyces cerevisiaeby Fusion of Host and Heterologous Enzymes

(2010) Line Albertsen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae

(2010) J. Rico et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of geranylgeraniol on overexpression of a prenyl diphosphate synthase fusion gene in Saccharomyces cerevisiae

(2010) Chikara Ohto et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Construction of a novel expression vector in Pseudonocardia autotrophica and its application to efficient biotransformation of compactin to pravastatin, a specific HMG-CoA reductase inhibitor

(2010) Yoshikazu Fujii et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Intracellular Acetyl Unit Transport in Fungal Carbon Metabolism

(2010) Karin Strijbis et al.   EUKARYOTIC CELL

Microbial hydrolysis of 7-xylosyl-10-deacetyltaxol to 10-deacetyltaxol

(2010) Kang Wang et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Analysis of Intact and Dissected Fungal Polyketide Synthase-Nonribosomal Peptide Synthetase in Vitro and inSaccharomyces cerevisiae

(2010) Wei Xu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Geraniol — A review of a commercially important fragrance material

(2010) W. Chen et al.   SOUTH AFRICAN JOURNAL OF BOTANY

BglBricks: A flexible standard for biological part assembly

(2010) J Christopher Anderson et al.   Journal of Biological Engineering

Metabolic Engineering of Saccharomyces cerevisiae for Astaxanthin Production and Oxidative Stress Tolerance

(2009) K. Ukibe et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Synthesis of Methyl Halides from Biomass Using Engineered Microbes

(2009) Travis S. Bayer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces cerevisiae

(2009) Emmanouil Trantas et al.   METABOLIC ENGINEERING

Spatiotemporal control of cell signalling using a light-switchable protein interaction

(2009) Anselm Levskaya et al.   NATURE

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Diversity-based, model-guided construction of synthetic gene networks with predicted functions

(2009) Tom Ellis et al.   NATURE BIOTECHNOLOGY

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzyme cascades activated on topologically programmed DNA scaffolds

(2009) Ofer I. Wilner et al.   Nature Nanotechnology

A fast and efficient translational control system for conditional expression of yeast genes

(2009) Peter Kötter et al.   NUCLEIC ACIDS RESEARCH

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast

(2009) C. Lartigue et al.   SCIENCE

Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production

(2008) Benedikt Engels et al.   METABOLIC ENGINEERING

Recent advances in the total synthesis of pharmaceutically relevant diterpenes

(2008) Torsten Busch et al.   NATURAL PRODUCT REPORTS

Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae

(2008) Kristy M Hawkins et al.   Nature Chemical Biology

Two nearly identical terpene synthases catalyze the formation of nerolidol and linalool in snapdragon flowers

(2008) Dinesh A. Nagegowda et al.   PLANT JOURNAL

Terpenoid biomaterials

(2008) Jörg Bohlmann et al.   PLANT JOURNAL

Uncovering cis Regulatory Codes Using Synthetic Promoter Shuffling

(2008) Ali Kinkhabwala et al.   PLoS One

Microbial production of plant benzylisoquinoline alkaloids

(2008) H. Minami et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE

Engineering microbial consortia: a new frontier in synthetic biology

(2008) Katie Brenner et al.   TRENDS IN BIOTECHNOLOGY

Engineering the Saccharomyces cerevisiae isoprenoid pathway for de novo production of aromatic monoterpenes in wine

(2007) Óscar Herrero et al.   METABOLIC ENGINEERING

Ameliorating effects of compounds derived from Salvia miltiorrhiza root extract on microcirculatory disturbance and target organ injury by ischemia and reperfusion

(2007) Jing-Yan Han et al.   PHARMACOLOGY & THERAPEUTICS

Combining Classical, Genetic, and Process Strategies for Improved Precursor-Directed Production of 6-Deoxyerythronolide B Analogues

(2004) Ruchir P. Desai et al.   BIOTECHNOLOGY PROGRESS