Phase separation on microtubules: from droplet formation to cellular function?

Structure of the Ndc80 complex and its interactions at the yeast kinetochore–microtubule interface

(2023) Jacob A. Zahm et al.   Open Biology

On the role of phase separation in the biogenesis of membraneless compartments

(2022) Andrea Musacchio   EMBO JOURNAL

Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells

(2022) Ivar Noordstra et al.   JOURNAL OF CELL SCIENCE

Lysate-based pipeline to characterize microtubule-associated proteins uncovers unique microtubule behaviours

(2022) A. S. Jijumon et al.   NATURE CELL BIOLOGY

Tau liquid–liquid phase separation in neurodegenerative diseases

(2022) Solomiia Boyko et al.   TRENDS IN CELL BIOLOGY

Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations

(2022) Maijia Liao et al.   MOLECULAR BIOLOGY OF THE CELL

Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography

(2022) Meng Zhang et al.   MOLECULAR CELL

A conceptual framework for understanding phase separation and addressing open questions and challenges

(2022) Tanja Mittag et al.   MOLECULAR CELL

CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation

(2022) Tsuyoshi Imasaki et al.   eLife

Self-assembly of pericentriolar material in interphase cells lacking centrioles

(2022) Fangrui Chen et al.   eLife

Microtubule lattice spacing governs cohesive envelope formation of tau family proteins

(2022) Valerie Siahaan et al.   Nature Chemical Biology

Phase separation of EB1 guides microtubule plus-end dynamics

(2022) Xiaoyu Song et al.   NATURE CELL BIOLOGY

Multivalency ensures persistence of a +TIP body at specialized microtubule ends

(2022) Sandro M. Meier et al.   NATURE CELL BIOLOGY

Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends

(2022) Renu Maan et al.   NATURE CELL BIOLOGY

1,6-Hexanediol, commonly used to dissolve liquid-liquid phase separated condensates, directly impairs kinase and phosphatase activities

(2021) Robert Düster et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin

(2021) Fernando R Balestra et al.   eLife

A hydrodynamic instability drives protein droplet formation on microtubules to nucleate branches

(2021) Sagar U. Setru et al.   Nature Physics

Suppression of liquid–liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells

(2021) Sergey V Ulianov et al.   NUCLEIC ACIDS RESEARCH

Tau forms oligomeric complexes on microtubules that are distinct from tau aggregates

(2021) Melina Theoni Gyparaki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid–liquid phase separation of tau: From molecular biophysics to physiology and disease

(2021) Sandeep K. Rai et al.   PROTEIN SCIENCE

Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly

(2021) Xueer Jiang et al.   JOURNAL OF CELL SCIENCE

SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation

(2021) Manjuan Zhang et al.   Journal of Molecular Cell Biology

Quantitative mapping of dense microtubule arrays in mammalian neurons

(2021) Eugene A Katrukha et al.   eLife

NuMA regulates mitotic spindle assembly, structural dynamics and function via phase separation

(2021) Mengjie Sun et al.   Nature Communications

A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion

(2020) Yiming Zheng et al.   NATURE CELL BIOLOGY

Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation

(2020) Matthew R. King et al.   Nature Communications

Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake

(2020) Arjen J. Jakobi et al.   Nature Communications

The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability

(2020) Johanna Roostalu et al.   eLife

Biomolecular Phase Separation: From Molecular Driving Forces to Macroscopic Properties

(2020) Gregory L. Dignon et al.   Annual Review of Physical Chemistry

LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation

(2020) Alexander von Appen et al.   NATURE

Composition-dependent thermodynamics of intracellular phase separation

(2020) Joshua A. Riback et al.   NATURE

Microtubules pull the strings: disordered sequences as efficient couplers of microtubule-generated force

(2020) Vladimir A. Volkov   Essays in Biochemistry

Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer

(2020) Zhong Y. Yeow et al.   NATURE

TRIM37 controls cancer-specific vulnerability to PLK4 inhibition

(2020) Franz Meitinger et al.   NATURE

Assembly of synaptic active zones requires phase separation of scaffold molecules

(2020) Nathan A. McDonald et al.   NATURE

The material state of centrosomes: lattice, liquid, or gel?

(2020) Jeffrey B Woodruff   CURRENT OPINION IN STRUCTURAL BIOLOGY

MAP7D2 Localizes to the Proximal Axon and Locally Promotes Kinesin-1-Mediated Cargo Transport into the Axon

(2019) Xingxiu Pan et al.   Cell Reports

MAP7 family proteins regulate kinesin-1 recruitment and activation

(2019) Peter Jan Hooikaas et al.   JOURNAL OF CELL BIOLOGY

Phase Separation and the Centrosome: A Fait Accompli?

(2019) Jordan W. Raff   TRENDS IN CELL BIOLOGY

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes

(2019) Chun So et al.   SCIENCE

Microtubules gate tau condensation to spatially regulate microtubule functions

(2019) Ruensern Tan et al.   NATURE CELL BIOLOGY

The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex

(2019) Prasad Trivedi et al.   NATURE CELL BIOLOGY

Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes

(2019) Valerie Siahaan et al.   NATURE CELL BIOLOGY

First-generation predictors of biological protein phase separation

(2019) Robert M Vernon et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

(2019) David T. McSwiggen et al.   GENES & DEVELOPMENT

The ERC1 scaffold protein implicated in cell motility drives the assembly of a liquid phase

(2019) Kristyna Sala et al.   Scientific Reports

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Kinesin expands and stabilizes the GDP-microtubule lattice

(2018) Daniel R. Peet et al.   Nature Nanotechnology

Structure-Function Relationship of the Bik1-Bim1 Complex

(2018) Marcel M. Stangier et al.   STRUCTURE

Competition between microtubule-associated proteins directs motor transport

(2018) Brigette Y. Monroy et al.   Nature Communications

Mapping multivalency in the CLIP-170–EB1 microtubule plus-end complex

(2018) Yaodong Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Kinesin-binding–triggered conformation switching of microtubules contributes to polarized transport

(2018) Tomohiro Shima et al.   JOURNAL OF CELL BIOLOGY

EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

(2017) Chao Yang et al.   JOURNAL OF CELL BIOLOGY

Microtubule nucleation: beyond the template

(2017) Johanna Roostalu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Differentiation between Oppositely Oriented Microtubules Controls Polarized Neuronal Transport

(2017) Roderick P. Tas et al.   NEURON

Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends

(2017) Anil Kumar et al.   STRUCTURE

Local Nucleation of Microtubule Bundles through Tubulin Concentration into a Condensed Tau Phase

(2017) Amayra Hernández-Vega et al.   Cell Reports

Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin

(2016) Alexandra Friese et al.   Nature Communications

Phase Transition of Spindle-Associated Protein Regulate Spindle Apparatus Assembly

(2015) Hao Jiang et al.   CELL

Control of microtubule organization and dynamics: two ends in the limelight

(2015) Anna Akhmanova et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Centrosome function and assembly in animal cells

(2015) Paul T. Conduit et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle

(2015) Faye M Nixon et al.   eLife

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Reconstitution of a hierarchical +TIP interaction network controlling microtubule end tracking of dynein

(2014) Christian Duellberg et al.   NATURE CELL BIOLOGY

Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation

(2014) Sandra Maday et al.   NEURON

A molecular mechanism of mitotic centrosome assembly in Drosophila

(2014) Paul T Conduit et al.   eLife

Amorphous no more: subdiffraction view of the pericentriolar material architecture

(2013) Vito Mennella et al.   TRENDS IN CELL BIOLOGY

EBs Recognize a Nucleotide-Dependent Structural Cap at Growing Microtubule Ends

(2012) Sebastian P. Maurer et al.   CELL

Estimating the Microtubule GTP Cap Size In Vivo

(2012) Dominique Seetapun et al.   CURRENT BIOLOGY

MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function

(2012) Thomas Metzger et al.   NATURE

Multimodal microtubule binding by the Ndc80 kinetochore complex

(2012) Gregory M Alushin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Beyond Stereospecificity: Liquids and Mesoscale Organization of Cytoplasm

(2011) Anthony A. Hyman et al.   DEVELOPMENTAL CELL

In Vitro Reconstitution of the Functional Interplay between MCAK and EB3 at Microtubule Plus Ends

(2010) Susana Montenegro Gouveia et al.   CURRENT BIOLOGY

Aurora B kinase controls the targeting of the Astrin–SKAP complex to bioriented kinetochores

(2010) Jens C. Schmidt et al.   JOURNAL OF CELL BIOLOGY

Regulation of Microtubule Dynamics by Bim1 and Bik1, the Budding Yeast Members of the EB1 and CLIP-170 Families of Plus-End Tracking Proteins

(2010) Kristina A. Blake-Hodek et al.   MOLECULAR BIOLOGY OF THE CELL

An EB1-Binding Motif Acts as a Microtubule Tip Localization Signal

(2009) Srinivas Honnappa et al.   CELL

Mammalian end binding proteins control persistent microtubule growth

(2009) Yulia Komarova et al.   JOURNAL OF CELL BIOLOGY

Phosphoregulation of the budding yeast EB1 homologue Bim1p by Aurora/Ipl1p

(2009) Tomasz Zimniak et al.   JOURNAL OF CELL BIOLOGY

Sensing Chromosome Bi-Orientation by Spatial Separation of Aurora B Kinase from Kinetochore Substrates

(2009) D. Liu et al.   SCIENCE

Shaping Fission Yeast with Microtubules

(2009) F. Chang et al.   Cold Spring Harbor Perspectives in Biology

Drosophila Ensconsin Promotes Productive Recruitment of Kinesin-1 to Microtubules

(2008) Hsin-Ho Sung et al.   DEVELOPMENTAL CELL

Dynamic behavior of GFP–CLIP-170 reveals fast protein turnover on microtubule plus ends

(2008) Katharina A. Dragestein et al.   JOURNAL OF CELL BIOLOGY

EB1 regulates microtubule dynamics and tubulin sheet closure in vitro

(2008) Benjamin Vitre et al.   NATURE CELL BIOLOGY

Tracking the ends: a dynamic protein network controls the fate of microtubule tips

(2008) Anna Akhmanova et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Capturing protein tails by CAP-Gly domains

(2008) Michel O. Steinmetz et al.   TRENDS IN BIOCHEMICAL SCIENCES