Optical Approaches for Investigating Neuromodulation and G Protein–Coupled Receptor Signaling

Photoswitchable Serotonins for Optical Control of the 5‐HT2A Receptor

(2022) Johannes Morstein et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enlightening the “Spirit Molecule”: Photomodulation of the 5‐HT 2A Receptor by a Light‐Controllable N , N ‐Dimethyltryptamine Derivative

(2022) Hubert Gerwe et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Fluorescence Imaging of Neural Activity, Neurochemical Dynamics, and Drug-Specific Receptor Conformation with Genetically Encoded Sensors

(2022) Chunyang Dong et al.   Annual Review of Neuroscience

Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling

(2022) Selma E. Anton et al.   CELL

Optical control of Class A G protein-coupled receptors with photoswitchable ligands

(2022) Maikel Wijtmans et al.   CURRENT OPINION IN PHARMACOLOGY

Advances in tethered photopharmacology for precise optical control of signaling proteins

(2022) Johannes Broichhagen et al.   CURRENT OPINION IN PHARMACOLOGY

β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice

(2022) Zhuogui Lei et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Optogenetics at the presynapse

(2022) Benjamin R. Rost et al.   NATURE NEUROSCIENCE

Conformational selection guides β-arrestin recruitment at a biased G protein–coupled receptor

(2022) Andrew B. Kleist et al.   SCIENCE

β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells

(2022) Raphael S. Haider et al.   Nature Communications

Control of Gα q signaling dynamics and GPCR cross-talk by GRKs

(2022) Guoqing Xiang et al.   Science Advances

Optical Control of Cannabinoid Receptor 2-Mediated Ca2+ Release Enabled by Synthesis of Photoswitchable Probes

(2021) Roman C. Sarott et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Using a bistable animal opsin for switchable and scalable optogenetic inhibition of neurons

(2021) Jessica Rodgers et al.   EMBO REPORTS

Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin

(2021) Mathias Mahn et al.   NEURON

A photoswitchable GPCR-based opsin for presynaptic inhibition

(2021) Bryan A. Copits et al.   NEURON

Selective Photoswitchable Allosteric Agonist of a G Protein-Coupled Receptor

(2021) Prashant Donthamsetti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A locus coeruleus to dentate gyrus noradrenergic circuit modulates aversive contextual processing

(2021) Dong-oh Seo et al.   NEURON

Cell specific photoswitchable agonist for reversible control of endogenous dopamine receptors

(2021) Prashant Donthamsetti et al.   Nature Communications

Reverse optogenetics of G protein signaling by zebrafish non-visual opsin Opn7b for synchronization of neuronal networks

(2021) Raziye Karapinar et al.   Nature Communications

An endogenous opioid circuit determines state-dependent reward consumption

(2021) Daniel C. Castro et al.   NATURE

Letting the little light of mind shine: Advances and future directions in neurochemical detection

(2021) Nikki Tjahjono et al.   NEUROSCIENCE RESEARCH

Heterotrimeric Gq proteins as therapeutic targets?

(2020) Evi Kostenis et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure-based development of caged dopamine D2/D3 receptor antagonists

(2020) Marie Gienger et al.   Scientific Reports

Agonist-selective recruitment of engineered protein probes and of GRK2 by opioid receptors in living cells

(2020) Miriam Stoeber et al.   eLife

Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System

(2020) Avery C. Hunker et al.   Cell Reports

Conformational Basis of G Protein-Coupled Receptor Signaling Versatility

(2020) Laura M. Wingler et al.   TRENDS IN CELL BIOLOGY

Optical Mapping of cAMP Signaling at the Nanometer Scale

(2020) Andreas Bock et al.   CELL

Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein

(2020) Mikel Garcia-Marcos et al.   eLife

Restoration of high-sensitivity and adapting vision with a cone opsin

(2019) Michael H. Berry et al.   Nature Communications

GPCR photopharmacology

(2019) Maria Ricart-Ortega et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Revisiting and Redesigning Light-Activated Cyclic-Mononucleotide Phosphodiesterases

(2019) Robert Stabel et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetically Targeted Optical Control of an Endogenous G Protein-Coupled Receptor

(2019) Prashant C. Donthamsetti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Lamprey Parapinopsin (“UVLamP”): a bistable UV‐sensitive optogenetic switch for ultrafast control of GPCR pathways

(2019) Dennis Eickelbeck et al.   CHEMBIOCHEM

Photo-Release of 2-Arachidonoylglycerol in Live Cells

(2019) Aurelien Laguerre et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A toolbox of molecular photoswitches to modulate the CXCR3 chemokine receptor with light

(2019) Xavier Gómez-Santacana et al.   Beilstein Journal of Organic Chemistry

Branched Photoswitchable Tethered Ligands Enable Ultra-efficient Optical Control and Detection of G Protein-Coupled Receptors In Vivo

(2019) Amanda Acosta-Ruiz et al.   NEURON

Battery-free, lightweight, injectable microsystem for in vivo wireless pharmacology and optogenetics

(2019) Yi Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Photoswitching the efficacy of a small-molecule ligand for a peptidergic GPCR: from antagonism to agonism

(2018) Rob Leurs et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In Vivo Photopharmacology

(2018) Katharina Hüll et al.   CHEMICAL REVIEWS

Synthesis and Characterization of a Bidirectional Photoswitchable Antagonist Toolbox for Real-Time GPCR Photopharmacology

(2018) Niels J. Hauwert et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor

(2018) Keita Sato et al.   Nature Communications

Optical functionalization of human Class A orphan G-protein-coupled receptors

(2018) Maurizio Morri et al.   Nature Communications

Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 Å structure of the adenylyl cyclase domain

(2018) Ulrike Scheib et al.   Nature Communications

An all-trans-retinal-binding opsin peropsin as a potential dark-active and light-inactivated G protein-coupled receptor

(2018) Takashi Nagata et al.   Scientific Reports

Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers

(2018) Osamu Takenouchi et al.   Scientific Reports

Optogenetic Inhibition of Gαq Protein Signaling Reduces Calcium Oscillation Stochasticity

(2018) Pimkhuan Hannanta-anan et al.   ACS Synthetic Biology

SNAP-Tagged Nanobodies Enable Reversible Optical Control of a G Protein-Coupled Receptor via a Remotely Tethered Photoswitchable Ligand

(2018) Helen Farrants et al.   ACS Chemical Biology

Synthesis of Photoswitchable Δ9-Tetrahydrocannabinol Derivatives Enables Optical Control of Cannabinoid Receptor 1 Signaling

(2017) Matthias V. Westphal et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Optical Control of Dopamine Receptors Using a Photoswitchable Tethered Inverse Agonist

(2017) Prashant C. Donthamsetti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Silencing Neurons: Tools, Applications, and Experimental Constraints

(2017) J. Simon Wiegert et al.   NEURON

Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors

(2017) Joshua Levitz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deconstructing behavioral neuropharmacology with cellular specificity

(2017) Brenda C. Shields et al.   SCIENCE

Optical control of cell signaling by single-chain photoswitchable kinases

(2017) Xin X. Zhou et al.   SCIENCE

Optical control of GPR40 signalling in pancreatic β-cells

(2017) James Allen Frank et al.   Chemical Science

Structural basis of arrestin-3 activation and signaling

(2017) Qiuyan Chen et al.   Nature Communications

A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs

(2017) Raquel M. Melero-Fernandez de Mera et al.   Nature Communications

Optical control of pain in vivo with a photoactive mGlu5 receptor negative allosteric modulator

(2017) Joan Font et al.   eLife

Allosteric Optical Control of a Class B G-Protein-Coupled Receptor

(2016) Johannes Broichhagen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optogenetic user s guide to Opto-GPCRs

(2016) Sonja Kleinlogel   Frontiers in Bioscience-Landmark

Getting into the brain: liposome-based strategies for effective drug delivery across the blood–brain barrier

(2016) Débora Vieira et al.   International Journal of Nanomedicine

Using melanopsin to study G protein signaling in cortical neurons

(2016) K. M. McGregor et al.   JOURNAL OF NEUROPHYSIOLOGY

A bacterial phytochrome-based optogenetic system controllable with near-infrared light

(2016) Andrii A Kaberniuk et al.   NATURE METHODS

Biophysical constraints of optogenetic inhibition at presynaptic terminals

(2016) Mathias Mahn et al.   NATURE NEUROSCIENCE

Chemogenetic and Optogenetic Activation of Gαs Signaling in the Basolateral Amygdala Induces Acute and Social Anxiety-Like States

(2016) Edward R Siuda et al.   NEUROPSYCHOPHARMACOLOGY

Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems

(2016) Gaigai Yu et al.   Scientific Reports

AzoCholine Enables Optical Control of Alpha 7 Nicotinic Acetylcholine Receptors in Neural Networks

(2015) Arunas Damijonaitis et al.   ACS Chemical Neuroscience

Optical Control of Insulin Secretion Using an Incretin Switch

(2015) Johannes Broichhagen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics

(2015) Jae-Woong Jeong et al.   CELL

Optogenetic activation of intracellular adenosine A2A receptor signaling in the hippocampus is sufficient to trigger CREB phosphorylation and impair memory

(2015) P Li et al.   MOLECULAR PSYCHIATRY

Optogenetics: 10 years of microbial opsins in neuroscience

(2015) Karl Deisseroth   NATURE NEUROSCIENCE

Spatiotemporal Control of Opioid Signaling and Behavior

(2015) Edward R. Siuda et al.   NEURON

Optogenetic control of intracellular signaling pathways

(2015) Kai Zhang et al.   TRENDS IN BIOTECHNOLOGY

Optodynamic simulation of β-adrenergic receptor signalling

(2015) Edward R. Siuda et al.   Nature Communications

Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering

(2015) L. J. Bugaj et al.   Nature Communications

Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool

(2015) Michiel van Wyk et al.   PLOS BIOLOGY

Orthogonal Optical Control of a G Protein-Coupled Receptor with a SNAP-Tethered Photochromic Ligand

(2015) Johannes Broichhagen et al.   ACS Central Science

Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates

(2015) Emi Kawano-Yamashita et al.   PLoS One

A Photochromic Agonist for μ-Opioid Receptors

(2014) Matthias Schönberger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Photomodulation of G Protein-Coupled Adenosine Receptors by a Novel Light-Switchable Ligand

(2014) María Isabel Bahamonde et al.   BIOCONJUGATE CHEMISTRY

Natural Neural Projection Dynamics Underlying Social Behavior

(2014) Lisa A. Gunaydin et al.   CELL

Subcellular optogenetics - controlling signaling and single-cell behavior

(2014) W. K. A. Karunarathne et al.   JOURNAL OF CELL SCIENCE

Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration

(2014) Patrick R. O'Neill et al.   MOLECULAR BIOLOGY OF THE CELL

An allosteric modulator to control endogenous G protein-coupled receptors with light

(2014) Silvia Pittolo et al.   Nature Chemical Biology

Vertebrate Cone Opsins Enable Sustained and Highly Sensitive Rapid Control of G i/o Signaling in Anxiety Circuitry

(2014) Olivia A. Masseck et al.   NEURON

Optogenetic control of chemokine receptor signal and T-cell migration

(2014) Y. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of a red-light-activated human cAMP/cGMP-specific phosphodiesterase

(2014) C. Gasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine

(2013) Roberto Araya et al.   ACS Chemical Neuroscience

Nanotools for Neuroscience and Brain Activity Mapping

(2013) A. Paul Alivisatos et al.   ACS Nano

Design and functional evaluation of an optically active μ-opioid receptor

(2013) Philip A. Barish et al.   EUROPEAN JOURNAL OF PHARMACOLOGY

Conformational Flexibility and Structural Dynamics in GPCR-Mediated G Protein Activation: A Perspective

(2013) Anita M. Preininger et al.   JOURNAL OF MOLECULAR BIOLOGY

Optogenetic protein clustering and signaling activation in mammalian cells

(2013) Lukasz J Bugaj et al.   NATURE METHODS

Optical control of metabotropic glutamate receptors

(2013) Joshua Levitz et al.   NATURE NEUROSCIENCE

Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis

(2013) Toshiyuki Kakumoto et al.   PLoS One

Homologs of vertebrate Opn3 potentially serve as a light sensor in nonphotoreceptive tissue

(2013) M. Koyanagi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Control over molecular motion using thecis–transphotoisomerization of the azo group

(2012) Estíbaliz Merino et al.   Beilstein Journal of Organic Chemistry

LOV to BLUF: Flavoprotein Contributions to the Optogenetic Toolkit

(2012) John M. Christie et al.   Molecular Plant

Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors

(2012) Ivan Tochitsky et al.   Nature Chemistry

Photoactivatable Neuropeptides for Spatiotemporally Precise Delivery of Opioids in Neural Tissue

(2012) Matthew R. Banghart et al.   NEURON

Ectopic expression of melanopsin in orexin/hypocretin neurons enables control of wakefulness of mice in vivo by blue light

(2012) Tomomi Tsunematsu et al.   NEUROSCIENCE RESEARCH

Molecular Mechanisms of Opioid Receptor-dependent Signaling and Behavior

(2011) Ream Al-Hasani et al.   ANESTHESIOLOGY

The Development and Application of Optogenetics

(2011) Lief Fenno et al.   Annual Review of Neuroscience

Azobenzene photoswitches for biomolecules

(2011) Andrew A. Beharry et al.   CHEMICAL SOCIETY REVIEWS

Photoisomerization in different classes of azobenzene

(2011) H. M. Dhammika Bandara et al.   CHEMICAL SOCIETY REVIEWS

Light Modulation of Cellular cAMP by a Small Bacterial Photoactivated Adenylyl Cyclase, bPAC, of the Soil BacteriumBeggiatoa

(2010) Manuela Stierl et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Substitution of 5-HT1AReceptor Signaling by a Light-activated G Protein-coupled Receptor

(2010) Eugene Oh et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Rapid blue-light–mediated induction of protein interactions in living cells

(2010) Matthew J Kennedy et al.   NATURE METHODS

Opn5 is a UV-sensitive bistable pigment that couples with Gi subtype of G protein

(2010) T. Yamashita et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Kinase cascades and ligand-directed signaling at the kappa opioid receptor

(2010) Michael R. Bruchas et al.   PSYCHOPHARMACOLOGY

Spatiotemporal control of cell signalling using a light-switchable protein interaction

(2009) Anselm Levskaya et al.   NATURE

Temporally precise in vivo control of intracellular signalling

(2009) Raag D. Airan et al.   NATURE

The structure and function of G-protein-coupled receptors

(2009) Daniel M. Rosenbaum et al.   NATURE

A genetically encoded photoactivatable Rac controls the motility of living cells

(2009) Yi I. Wu et al.   NATURE

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Light Activation of the LOV Protein Vivid Generates a Rapidly Exchanging Dimer†‡

(2008) Brian D. Zoltowski et al.   BIOCHEMISTRY

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

Rod and cone photoreceptors: Molecular basis of the difference in their physiology

(2008) Satoru Kawamura et al.   COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY

Estimation of the available free energy in a LOV2-Jα photoswitch

(2008) Xiaolan Yao et al.   Nature Chemical Biology

Photo-control of peptide conformation on a timescale of seconds with a conformationally constrained, blue-absorbing, photo-switchable linker

(2008) Andrew A. Beharry et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Restoration of visual function in retinal degeneration mice by ectopic expression of melanopsin

(2008) B. Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distinct conformational changes in  -arrestin report biased agonism at seven-transmembrane receptors

(2008) A. K. Shukla et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Photoexcited CRY2 Interacts with CIB1 to Regulate Transcription and Floral Initiation in Arabidopsis

(2008) H. Liu et al.   SCIENCE