A library approach for the de novo high-throughput isolation of humanized VHH domains with favorable developability properties following camelid immunization

The RESP AI model accelerates the identification of tight-binding antibodies

(2023) Jonathan Parkinson et al.   Nature Communications

Design of antibody variable fragments with reduced reactivity to preexisting anti-drug antibodies

(2023) Maria U. Johansson et al.   mAbs

Trends in industrialization of biotherapeutics: a survey of product characteristics of 89 antibody-based biotherapeutics

(2023) Kyle P. Martin et al.   mAbs

NKp46 ‐specific single domain antibodies enable facile engineering of various potent NK cell engager formats

(2023) Britta Lipinski et al.   PROTEIN SCIENCE

Generation and engineering of potent single domain antibody-based bispecific IL-18 mimetics resistant to IL-18BP decoy receptor inhibition

(2023) Britta Lipinski et al.   mAbs

Envafolimab: First Approval

(2022) Anthony Markham   DRUGS

Beyond bispecificity: Controlled Fab arm exchange for the generation of antibodies with multiple specificities

(2022) Desislava Yanakieva et al.   mAbs

Grabbing the Bull by Both Horns: Bovine Ultralong CDR-H3 Paratopes Enable Engineering of ‘Almost Natural’ Common Light Chain Bispecific Antibodies Suitable For Effector Cell Redirection

(2022) Daniel Klewinghaus et al.   Frontiers in Immunology

Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space

(2022) Emily K. Makowski et al.   Nature Communications

Multifunctional NK Cell–Engaging Antibodies Targeting EGFR and NKp30 Elicit Efficient Tumor Cell Killing and Proinflammatory Cytokine Release

(2022) Katja Klausz et al.   JOURNAL OF IMMUNOLOGY

Ozoralizumab: First Approval

(2022) Susan J. Keam   DRUGS

Antibodies to watch in 2023

(2022) Hélène Kaplon et al.   mAbs

Biopharmaceutical benchmarks 2022

(2022) Gary Walsh et al.   NATURE BIOTECHNOLOGY

Effect of Humanizing Mutations on the Stability of the Llama Single-Domain Variable Region

(2021) Miguel A. Soler et al.   Biomolecules

Immunogenicity and humanization of single‐domain antibodies

(2021) Martin A. Rossotti et al.   FEBS Journal

Antibody design using LSTM based deep generative model from phage display library for affinity maturation

(2021) Koichiro Saka et al.   Scientific Reports

Antibody display technologies: selecting the cream of the crop

(2021) Bernhard Valldorf et al.   BIOLOGICAL CHEMISTRY

Optimization of therapeutic antibodies by predicting antigen specificity from antibody sequence via deep learning

(2021) Derek M. Mason et al.   Nature Biomedical Engineering

Natural killer cell engagers in cancer immunotherapy: Next generation of immuno‐oncology treatments

(2021) Olivier Demaria et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

A structure-based engineering approach to abrogate pre-existing antibody binding to biotherapeutics

(2021) Joanne Lin et al.   PLoS One

Milking the Cow: Cattle-Derived Chimeric Ultralong CDR-H3 Antibodies and Their Engineered CDR-H3-Only Knobbody Counterparts Targeting Epidermal Growth Factor Receptor Elicit Potent NK Cell-Mediated Cytotoxicity

(2021) Lukas Pekar et al.   Frontiers in Immunology

A One-Step Process for the Construction of Phage Display scFv and VHH Libraries

(2020) Carolin Sellmann et al.   MOLECULAR BIOTECHNOLOGY

Affinity Maturation of B7-H6 Translates into Enhanced NK Cell–Mediated Tumor Cell Lysis and Improved Proinflammatory Cytokine Release of Bispecific Immunoligands via NKp30 Engagement

(2020) Lukas Pekar et al.   JOURNAL OF IMMUNOLOGY

An improved yeast surface display platform for the screening of nanobody immune libraries

(2019) Tomasz Uchański et al.   Scientific Reports

Next-generation sequencing-guided identification and reconstruction of antibody CDR combinations from phage selection outputs

(2019) Kris Barreto et al.   NUCLEIC ACIDS RESEARCH

Multifunctional Natural Killer Cell Engagers Targeting NKp46 Trigger Protective Tumor Immunity

(2019) Laurent Gauthier et al.   CELL

Antibody Complementarity Determining Region Design Using High-Capacity Machine Learning

(2019) Ge Liu et al.   BIOINFORMATICS

Understanding and overcoming trade-offs between antibody affinity, specificity, stability and solubility

(2018) Lilia A. Rabia et al.   BIOCHEMICAL ENGINEERING JOURNAL

OUP accepted manuscript

(2018)   PROTEIN ENGINEERING DESIGN & SELECTION

Caplacizumab: First Global Approval

(2018) Sean Duggan   DRUGS

Deamidation and isomerization liability analysis of 131 clinical-stage antibodies

(2018) Xiaojun Lu et al.   mAbs

Biophysical properties of the clinical-stage antibody landscape

(2017) Tushar Jain et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics

(2017) Peter Bannas et al.   Frontiers in Immunology

Development of purification processes for fully human bispecific antibodies based upon modification of protein A binding avidity

(2016) Andrew D. Tustian et al.   mAbs

NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies

(2016) Sandrine Moutel et al.   eLife

Single-domain antibodies for biomedical applications

(2015) Simon Krah et al.   IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY

Influence of molecular size on tissue distribution of antibody fragments

(2015) Zhe Li et al.   mAbs

Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies

(2015) Dongmei Hu et al.   PLoS One

A general protocol for the generation of Nanobodies for structural biology

(2014) Els Pardon et al.   Nature Protocols

Therapeutic antibody engineering by high efficiency cell screening

(2013) Achim Doerner et al.   FEBS LETTERS

High-throughput screening for developability during early-stage antibody discovery using self-interaction nanoparticle spectroscopy

(2013) Yuqi Liu et al.   mAbs

High-Throughput Analysis of Concentration-Dependent Antibody Self-Association

(2011) Shantanu V. Sule et al.   BIOPHYSICAL JOURNAL

A Novel Promiscuous Class of Camelid Single-Domain Antibody Contributes to the Antigen-Binding Repertoire

(2010) N. Deschacht et al.   JOURNAL OF IMMUNOLOGY

An improved yeast transformation method for the generation of very large human antibody libraries

(2010) Lorenzo Benatuil et al.   PROTEIN ENGINEERING DESIGN & SELECTION

SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies†

(2010) Jonathan H. Davis et al.   PROTEIN ENGINEERING DESIGN & SELECTION

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY