Tissue clearing and immunostaining to visualize the spatial organization of vasculature and tumor cells in mouse liver

VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology

(2022) Akanksha Bhargava et al.   NATURE METHODS

Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice

(2022) Stefanie Bobe et al.   Frontiers in Cell and Developmental Biology

Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation

(2021) Julian Hofmann et al.   Frontiers in Immunology

CUBIC-plus: An optimized method for rapid tissue clearing and decolorization

(2021) Zhengyu Ren et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Tissue clearing and 3D imaging – putting immune cells into context

(2021) Julian Hofmann et al.   JOURNAL OF CELL SCIENCE

Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect

(2021) R. C. M. Vulders et al.   PLoS One

Tissue clearing and its applications in neuroscience

(2020) Hiroki R. Ueda et al.   NATURE REVIEWS NEUROSCIENCE

Biomedical applications of tissue clearing and three-dimensional imaging in health and disease

(2020) Maria Victoria Gómez-Gaviro et al.   iScience

Tissue clearing technique: Recent progress and biomedical applications

(2020) Ting Tian et al.   JOURNAL OF ANATOMY

3D histopathology of human tumours by fast clearing and ultramicroscopy

(2020) Inna Sabdyusheva Litschauer et al.   Scientific Reports

Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH

(2020) Hendrik A. Messal et al.   Nature Protocols

Intraclonal Plasticity in Mammary Tumors Revealed through Large-Scale Single-Cell Resolution 3D Imaging

(2019) Anne C. Rios et al.   CANCER CELL

Assessing micrometastases as a target for nanoparticles using 3D microscopy and machine learning

(2019) Benjamin R. Kingston et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy

(2018) Nobuyuki Tanaka et al.   BRITISH JOURNAL OF CANCER

Tissue clearing of both hard and soft tissue organs with the PEGASOS method

(2018) Dian Jing et al.   CELL RESEARCH

Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)

(2018) Roni Oren et al.   Scientific Reports

Multiplex Three-Dimensional Mapping of Macromolecular Drug Distribution in the Tumor Microenvironment

(2018) Steve Seung-Young Lee et al.   MOLECULAR CANCER THERAPEUTICS

Heterogeneity of macrophage infiltration and therapeutic response in lung carcinoma revealed by 3D organ imaging

(2017) Michael F. Cuccarese et al.   Nature Communications

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution

(2017) Shimpei I. Kubota et al.   Cell Reports

Neuroscience in the third dimension: shedding new light on the brain with tissue clearing

(2017) Robin J. Vigouroux et al.   Molecular Brain

Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity

(2017) Nobuyuki Tanaka et al.   Nature Biomedical Engineering

UbasM: An effective balanced optical clearing method for intact biomedical imaging

(2017) Lingling Chen et al.   Scientific Reports

PanIN-associated pericyte, glial, and islet remodeling in mice revealed by 3D pancreatic duct lesion histology

(2016) Pei-Yu Lin et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Multiplexed Intact-Tissue Transcriptional Analysis at Cellular Resolution

(2016) Emily Lauren Sylwestrak et al.   CELL

Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing

(2016) Sheel Shah et al.   DEVELOPMENT

Deep tissue imaging: a review from a preclinical cancer research perspective

(2016) Annette Feuchtinger et al.   HISTOCHEMISTRY AND CELL BIOLOGY

Three-dimensional microscopic analysis of clinical prostate specimens

(2016) Martin E van Royen et al.   HISTOPATHOLOGY

Clearing and Labeling Techniques for Large-Scale Biological Tissues

(2016) Jinyoung Seo et al.   MOLECULES AND CELLS

Shrinkage-mediated imaging of entire organs and organisms using uDISCO

(2016) Chenchen Pan et al.   NATURE METHODS

A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue

(2016) Adriano Azaripour et al.   PROGRESS IN HISTOCHEMISTRY AND CYTOCHEMISTRY

Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging

(2015) Etsuo A Susaki et al.   Nature Protocols

Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response

(2015) Michael Dobosz et al.   NEOPLASIA

Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing

(2014) Bin Yang et al.   CELL

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging

(2014) Nicolas Renier et al.   CELL

Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization

(2014) Kazuki Tainaka et al.   CELL

Advanced CLARITY for rapid and high-resolution imaging of intact tissues

(2014) Raju Tomer et al.   Nature Protocols

Concomitant Notch activation and p53 deletion trigger epithelial-to-mesenchymal transition and metastasis in mouse gut

(2014) Maia Chanrion et al.   Nature Communications

Micro-CT Imaging of Tumor Angiogenesis

(2013) Josef Ehling et al.   AMERICAN JOURNAL OF PATHOLOGY

3-D Visualization and Quantitation of Microvessels in Transparent Human Colorectal Carcinoma

(2013) Yuan-An Liu et al.   PLoS One

Three-dimensional imaging of solvent-cleared organs using 3DISCO

(2012) Ali Ertürk et al.   Nature Protocols