Overview of Amino Acid Modifications by Iron- and α-Ketoglutarate-Dependent Enzymes

Comprehensive Structure–Activity Relationship Studies of Cepafungin Enabled by Biocatalytic C–H Oxidations

(2023) Alexander Amatuni et al.   ACS Central Science

Directed Evolution of an Iron(II)‐ and α‐Ketoglutarate‐Dependent Dioxygenase for Site‐Selective Azidation of Unactivated Aliphatic C−H Bonds**

(2023) Christian A. Gomez et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Discovery of a Cryptic Nitro Intermediate in the Biosynthesis of the 3-(trans-2′-Aminocyclopropyl)alanine Moiety of Belactosin A

(2022) Alicia Engelbrecht et al.   ORGANIC LETTERS

Discovery of New Fe(II)/α-Ketoglutarate-Dependent Dioxygenases for Oxidation of l-Proline

(2022) Erika Tassano et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria

(2022) Stella T. Lima et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biosynthesis of Glidomides and Elucidation of Different Mechanisms for Formation of β‐OH Amino Acid Building Blocks

(2022) Hanna Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Molecular Insights into the Regioselectivity of the Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Catalyzed C–H Hydroxylation of Amino Acids

(2022) Lunjie Wu et al.   ACS Catalysis

Stereoselectivity and Substrate Specificity of the Fe(II)/α-Ketoglutarate-Dependent Oxygenase TqaL

(2022) Hui Tao et al.   Journal of the American Chemical Society

Modular Chemoenzymatic Synthesis of GE81112 B1 and Related Analogues Enables Elucidation of Its Key Pharmacophores

(2021) Christian R. Zwick et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biosynthesis and Mechanism of Action of the Cell Wall Targeting Antibiotic Hypeptin

(2021) Daniel A. Wirtz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Aziridine formation by a Fe(II)/α‐ketoglutarate dependent oxygenase and 2‐aminoisobutyrate biosynthesis in fungi

(2021) Ikuro Abe et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Regiodivergent biocatalytic hydroxylation of l-Glutamine facilitated by characterization of Non-Heme dioxygenases from Non-Ribosomal peptide biosyntheses

(2021) Hans Renata et al.   TETRAHEDRON

In vivo and in vitro reconstitution of unique key steps in cystobactamid antibiotic biosynthesis

(2021) Sebastian Groß et al.   Nature Communications

Modulating Chemoselectivity in a Fe(II)/α-Ketoglutarate-Dependent Dioxygenase for the Oxidative Modification of a Nonproteinogenic Amino Acid

(2021) Fabian Meyer et al.   ACS Catalysis

Enzymatic Synthesis of l - threo -β-Hydroxy-α-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Sulfobacillus thermotolerans Y0017

(2021) Ryotaro Hara et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Re‐Programming and Optimization of a L ‐Proline cis ‐4‐Hydroxylase for the cis ‐3‐Halogenation of its Native Substrate

(2021) Athena Papadopoulou et al.   ChemCatChem

Identification of Cyclopropane Formation in the Biosyntheses of Hormaomycins and Belactosins: Sequential Nitration and Cyclopropanation by Metalloenzymes

(2021) Xiaojun Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Stereodivergent Nitrocyclopropane Formation during Biosynthesis of Belactosins and Hormaomycins

(2021) Shotaro Shimo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reaction pathway engineering converts a radical hydroxylase into a halogenase

(2021) Monica E. Neugebauer et al.   Nature Chemical Biology

Exploring the biocatalytic potential of Fe/α‐ketoglutarate dependent halogenases

(2020) Moritz Voss et al.   CHEMISTRY-A EUROPEAN JOURNAL

Ibotenic Acid Biosynthesis in the Fly Agaric Is Initiated by Glutamate Hydroxylation

(2020) Michael Müller et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cofactor Generation Cascade for α-Ketoglutarate and Fe(II)-Dependent Dioxygenases

(2020) Florian Busch et al.   ACS Sustainable Chemistry & Engineering

Halogenases: structures and functions

(2020) Hannes Ludewig et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Industrial Application of 2-Oxoglutarate-Dependent Oxygenases

(2019) Christin Peters et al.   Catalysts

Discovery of a pathway for terminal-alkyne amino acid biosynthesis

(2019) J. A. Marchand et al.   NATURE

A family of radical halogenases for the engineering of amino-acid-based products

(2019) Monica E. Neugebauer et al.   Nature Chemical Biology

Characterization of a Citrulline 4-Hydroxylase from Nonribosomal Peptide GE81112 Biosynthesis and Engineering of Its Substrate Specificity for the Chemoenzymatic Synthesis of Enduracididine

(2019) Christian R. Zwick et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways

(2019) Rémi Zallot et al.   BIOCHEMISTRY

Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles

(2019) Tristan J. Smart et al.   BIOORGANIC CHEMISTRY

Site-Selective C–H Halogenation Using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling

(2019) Brian F. Fisher et al.   ACS Central Science

Biosynthetic Pathways to Nonproteinogenic α-Amino Acids

(2019) Jason B. Hedges et al.   CHEMICAL REVIEWS

In Vitro Biosynthesis of the Nonproteinogenic Amino Acid Methoxyvinylglycine

(2018) Jon B. Patteson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Remote C–H Hydroxylation by an α-Ketoglutarate-Dependent Dioxygenase Enables Efficient Chemoenzymatic Synthesis of Manzacidin C and Proline Analogs

(2018) Christian R. Zwick et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genome Mining and Assembly-Line Biosynthesis of the UCS1025A Pyrrolizidinone Family of Fungal Alkaloids

(2018) Li Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Recent examples of α-ketoglutarate-dependent mononuclear non-haem iron enzymes in natural product biosyntheses

(2018) Shu-Shan Gao et al.   NATURAL PRODUCT REPORTS

Cryptic Production of trans -3-Hydroxyproline in Echinocandin B Biosynthesis

(2018) Johanna Mattay et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A one-pot chemoenzymatic synthesis of (2S, 4R)-4-methylproline enables the first total synthesis of antiviral lipopeptide cavinafungin B

(2018) Christian R. Zwick et al.   TETRAHEDRON

A Comparative Review on the Catalytic Mechanism of Nonheme Iron Hydroxylases and Halogenases

(2018) Amy Timmins et al.   Catalysts

Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity

(2017) Peer Lukat et al.   Chemical Science

Novel Enzyme Family Found in Filamentous Fungi Catalyzingtrans-4-Hydroxylation of l-Pipecolic Acid

(2016) Makoto Hibi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Ferrous iron and α-ketoglutarate-dependent dioxygenases in the biosynthesis of microbial natural products

(2016) Long-Fei Wu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Multi-Enzymatic Synthesis of Optically Pure β-Hydroxy α-Amino Acids

(2014) Makoto Hibi et al.   ADVANCED SYNTHESIS & CATALYSIS

Biosynthesis of Streptolidine Involved Two Unexpected Intermediates Produced by a Dihydroxylase and a Cyclase through Unusual Mechanisms

(2014) Chin-Yuan Chang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Characteristics and biotechnology applications of aliphatic amino acid hydroxylases belonging to the Fe(II)/α-ketoglutarate-dependent dioxygenase superfamily

(2014) Makoto Hibi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthesis of Mono- and Dihydroxylated Amino Acids with New α-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of CH Bonds

(2014) Damien Baud et al.   ChemCatChem

Identification and characterization of 2-oxoglutarate-dependent dioxygenases catalyzing selective cis-hydroxylation of proline and pipecolinic acid from actinomycetes

(2014) Ryotaro Hara et al.   JOURNAL OF BIOTECHNOLOGY

Direct nitration and azidation of aliphatic carbons by an iron-dependent halogenase

(2014) Megan L Matthews et al.   Nature Chemical Biology

Refined Regio- and Stereoselective Hydroxylation of l-Pipecolic Acid by Protein Engineering of l-Proline cis-4-Hydroxylase Based on the X-ray Crystal Structure

(2014) Kento Koketsu et al.   ACS Synthetic Biology

Recombinant protein expression in Escherichia coli: advances and challenges

(2014) Germán L. Rosano et al.   Frontiers in Microbiology

Nonproteinogenic Amino Acid Building Blocks for Nonribosomal Peptide and Hybrid Polyketide Scaffolds

(2013) Christopher T. Walsh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

EcdGHK Are Three Tailoring Iron Oxygenases for Amino Acid Building Blocks of the Echinocandin Scaffold

(2013) Wei Jiang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A novell-isoleucine-4′-dioxygenase andl-isoleucine dihydroxylation cascade inPantoea ananatis

(2013) Sergey V. Smirnov et al.   MicrobiologyOpen

l-Leucine 5-hydroxylase of Nostoc punctiforme is a novel type of Fe(II)/α-ketoglutarate-dependent dioxygenase that is useful as a biocatalyst

(2012) Makoto Hibi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A novel family of bacterial dioxygenases that catalyse the hydroxylation of free l-amino acids

(2012) Sergey V. Smirnov et al.   FEMS MICROBIOLOGY LETTERS

Halichonadins K and L, New Dimeric Sesquiterpenoids from a Sponge Halichondria sp.

(2012) Naonobu Tanaka et al.   ORGANIC LETTERS

Characterization of Bacillus thuringiensis l-Isoleucine Dioxygenase for Production of Useful Amino Acids

(2011) Makoto Hibi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4-hydroxyisoleucine

(2010) Sergey V. Smirnov et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterization of novel 2-oxoglutarate dependent dioxygenases converting l-proline to cis-4-hydroxy-l-proline

(2009) Ryotaro Hara et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Hydroxylation of l-proline to cis-3-hydroxy-l-proline by recombinant Escherichia coli expressing a synthetic l-proline-3-hydroxylase gene

(2009) Robert M. Johnston et al.   ENZYME AND MICROBIAL TECHNOLOGY

Stereospecific Synthesis ofthreo- anderythro-β-Hydroxyglutamic Acid During Kutzneride Biosynthesis

(2009) Matthias Strieker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ribosomal peptide natural products: bridging the ribosomal and nonribosomal worlds

(2009) John A. McIntosh et al.   NATURAL PRODUCT REPORTS

Stringency of the 2-His–1-Asp Active-Site Motif in Prolyl 4-Hydroxylase

(2009) Kelly L. Gorres et al.   PLoS One

β-Hydroxylation of the Aspartyl Residue in the Phytotoxin Syringomycin E: Characterization of Two Candidate Hydroxylases AspH and SyrP inPseudomonas syringae†

(2008) Gitanjali M. Singh et al.   BIOCHEMISTRY

Non-Heme Hydroxylase Engineering For Simple Enzymatic Synthesis ofL-threo-Hydroxyaspartic Acid

(2008) Matthias Strieker et al.   CHEMBIOCHEM

Biosynthesis of (−)-(1S,2R)-Allocoronamic Acyl Thioester by an FeII-Dependent Halogenase and a Cyclopropane-Forming Flavoprotein

(2008) Christopher S. Neumann et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY