Molecular Insights into the Regioselectivity of the Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Catalyzed C–H Hydroxylation of Amino Acids

Biosynthesis of Glidomides and Elucidation of Different Mechanisms for Formation of β‐OH Amino Acid Building Blocks

(2022) Hanna Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Single point mutant inverts stereoselectivity of a carbonyl reductase toward β‐ketoesters with enhanced activity

(2021) Aipeng Li et al.   CHEMISTRY-A EUROPEAN JOURNAL

Modular Chemoenzymatic Synthesis of GE81112 B1 and Related Analogues Enables Elucidation of Its Key Pharmacophores

(2021) Christian R. Zwick et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Active-site loop variations adjust activity and selectivity of the cumene dioxygenase

(2021) Peter M. Heinemann et al.   Nature Communications

Determinants for Substrate Recognition in the Glycyl Radical Enzyme Benzylsuccinate Synthase Revealed by Targeted Mutagenesis

(2021) Iryna Salii et al.   ACS Catalysis

N-acyl homoserine lactone molecules assisted quorum sensing: effects consequences and monitoring of bacteria talking in real life

(2021) Ömür Acet et al.   ARCHIVES OF MICROBIOLOGY

Engineered and Artificial Metalloenzymes for Selective C–H Functionalization

(2021) Xinkun Ren et al.   Current Opinion in Green and Sustainable Chemistry

Biocatalytic, Intermolecular C‐H Bond Functionalization for the Synthesis of Enantioenriched Amides

(2021) Soumitra Athavale et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Computer-aided understanding and engineering of enzymatic selectivity

(2021) Lunjie Wu et al.   BIOTECHNOLOGY ADVANCES

Molecular insights into the endoperoxide formation by Fe(II)/α-KG-dependent oxygenase NvfI

(2021) Takahiro Mori et al.   Nature Communications

Enzymatic Hydroxylations of sp3-Carbons

(2021) Judith Münch et al.   ACS Catalysis

From Cell-Free Protein Synthesis to Whole-Cell Biotransformation: Screening and Identification of Novel α-Ketoglutarate-Dependent Dioxygenases for Preparative-Scale Synthesis of Hydroxy-l-Lysine

(2021) Jascha Rolf et al.   Catalysts

Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule

(2021) Pamela Ruiz et al.   Frontiers in Cellular and Infection Microbiology

Single site mutations of glycosyltransferase with improved activity and regioselectivity for directed biosynthesis of unnatural protopanaxatriol-type ginsenoside product

(2021) Lu Zhao et al.   Molecular Catalysis

2-Ketoglutarate-Generated In Vitro Enzymatic Biosystem Facilitates Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Mediated C–H Bond Oxidation for (2s,3r,4s)-4-Hydroxyisoleucine Synthesis

(2020) Xiao-Ran Jing et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Engineering Bacillus subtilis Isoleucine Dioxygenase for Efficient Synthesis of (2S,3R,4S)-4-Hydroxyisoleucine

(2020) Ping Du et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Reshaping the Binding Pocket of Lysine Hydroxylase for Enhanced Activity

(2020) Fenghua Wang et al.   ACS Catalysis

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

(2019) Ge Qu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Regioselective and Stereoselective Hydroxylation of Free Amino Acids by a 2-Oxoglutarate-Dependent Dioxygenase from Kutzneria albida

(2019) Xiaoran Jing et al.   ACS Omega

Engineered disulfide bonds improve thermostability and activity of L‐isoleucine hydroxylase for efficient 4‐HIL production in Bacillus subtilis 168

(2019) Zhina Qiao et al.   ENGINEERING IN LIFE SCIENCES

A computational method for design of connected catalytic networks in proteins

(2019) Brian D. Weitzner et al.   PROTEIN SCIENCE

Directed evolution and site-specific mutagenesis of l-isoleucine dioxygenase derived from Bacillus weihenstephanensis

(2018) Sen Huang et al.   BIOTECHNOLOGY LETTERS

Adapting to oxygen: 3-Hydroxyanthrinilate 3,4-dioxygenase employs loop dynamics to accommodate two substrates with disparate polarities

(2018) Yu Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Amazing Diversity in Biochemical Roles of Fe(II)/2-Oxoglutarate Oxygenases

(2018) Caitlyn Q Herr et al.   TRENDS IN BIOCHEMICAL SCIENCES

Cloning and characterization of a new delta-specific l-leucine dioxygenase from Anabaena variabilis

(2018) Raquel S. Correia Cordeiro et al.   JOURNAL OF BIOTECHNOLOGY

Regioselectivity Engineering of Epoxide Hydrolase: Near-Perfect Enantioconvergence through a Single Site Mutation

(2018) Fu-Long Li et al.   ACS Catalysis

Automated Topology Builder Version 3.0: Prediction of Solvation Free Enthalpies in Water and Hexane

(2018) Martin Stroet et al.   Journal of Chemical Theory and Computation

Structural Studies based on two Lysine Dioxygenases with Distinct Regioselectivity Brings Insights Into Enzyme Specificity within the Clavaminate Synthase-Like Family

(2018) Karine Bastard et al.   Scientific Reports

Bioluminescent High-Throughput Succinate Detection Method for Monitoring the Activity of JMJC Histone Demethylases and Fe(II)/2-Oxoglutarate-Dependent Dioxygenases

(2017) Juliano Alves et al.   SLAS Discovery

A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles

(2016) Zhongyi Cheng et al.   Catalysis Science & Technology

A Multi-Enzymatic Cascade Reaction for the Stereoselective Production of γ-Oxyfunctionalyzed Amino Acids

(2016) Junichi Enoki et al.   Frontiers in Microbiology

Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases

(2015) Salette Martinez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Anatomy of enzyme channels

(2014) Lukáš Pravda et al.   BMC BIOINFORMATICS

Refined Regio- and Stereoselective Hydroxylation of l-Pipecolic Acid by Protein Engineering of l-Proline cis-4-Hydroxylase Based on the X-ray Crystal Structure

(2014) Kento Koketsu et al.   ACS Synthetic Biology

Crystal structure of a member of a novel family of dioxygenases (PF10014) reveals a conserved cupin fold and active site

(2013) Qingping Xu et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

l-Leucine 5-hydroxylase of Nostoc punctiforme is a novel type of Fe(II)/α-ketoglutarate-dependent dioxygenase that is useful as a biocatalyst

(2012) Makoto Hibi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

H++ 3.0: automating pK prediction and the preparation of biomolecular structures for atomistic molecular modeling and simulations

(2012) R. Anandakrishnan et al.   NUCLEIC ACIDS RESEARCH

REFMAC5 for the refinement of macromolecular crystal structures

(2011) Garib N. Murshudov et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Characterization of Bacillus thuringiensis l-Isoleucine Dioxygenase for Production of Useful Amino Acids

(2011) Makoto Hibi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Steric Factors Override Thermodynamic Driving Force in Regioselectivity of Proline Hydroxylation by Prolyl-4-hydroxylase Enzymes

(2010) Baharan Karamzadeh et al.   JOURNAL OF PHYSICAL CHEMISTRY A

Characterization of novel 2-oxoglutarate dependent dioxygenases converting l-proline to cis-4-hydroxy-l-proline

(2009) Ryotaro Hara et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A novel l-isoleucine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine

(2009) Tomohiro Kodera et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Hydroxylation of l-proline to cis-3-hydroxy-l-proline by recombinant Escherichia coli expressing a synthetic l-proline-3-hydroxylase gene

(2009) Robert M. Johnston et al.   ENZYME AND MICROBIAL TECHNOLOGY

AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading

(2009) Oleg Trott et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Stereospecific Synthesis ofthreo- anderythro-β-Hydroxyglutamic Acid During Kutzneride Biosynthesis

(2009) Matthias Strieker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY