Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiae

Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1

(2022) Zhenning Wang et al.   Science Advances

SPT6 functions in transcriptional pause/release via PAF1C recruitment

(2022) Yuki Aoi et al.   MOLECULAR CELL

Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT

(2022) Haruhiko Ehara et al.   SCIENCE

Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters

(2021) Theresa Endres et al.   MOLECULAR CELL

A CSB-PAF1C axis restores processive transcription elongation after DNA damage repair

(2021) Diana van den Heuvel et al.   Nature Communications

Integrative proteomics identifies thousands of distinct, multi-epitope, and high-affinity nanobodies

(2021) Yufei Xiang et al.   Cell Systems

Essential histone chaperones collaborate to regulate transcription and chromatin integrity

(2021) Olga Viktorovskaya et al.   GENES & DEVELOPMENT

The Paf1 Complex: A Keystone of Nuclear Regulation Operating at the Interface of Transcription and Chromatin

(2021) Alex M. Francette et al.   JOURNAL OF MOLECULAR BIOLOGY

The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions

(2021) Rajaraman Gopalakrishnan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Two distinct mechanisms of RNA polymerase II elongation stimulation in vivo

(2021) Kristina Žumer et al.   MOLECULAR CELL

Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination

(2021) Ashwin Narain et al.   MOLECULAR CELL

Biochemical insights into Paf1 complex–induced stimulation of Rad6/Bre1-mediated H2B monoubiquitination

(2021) Feilong Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Opposing functions of the Hda1 complex and histone H2B mono-ubiquitylation in regulating cryptic transcription in Saccharomyces cerevisiae

(2021) Margaret K Shirra et al.   G3-Genes Genomes Genetics

Structure of complete Pol II–DSIF–PAF–SPT6 transcription complex reveals RTF1 allosteric activation

(2020) Seychelle M. Vos et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CDK12 globally stimulates RNA polymerase II transcription elongation and carboxyl-terminal domain phosphorylation

(2020) Michael Tellier et al.   NUCLEIC ACIDS RESEARCH

Mechanisms of Transcription Elongation Factor DSIF (Spt4–Spt5)

(2020) Tim-Michael Decker   JOURNAL OF MOLECULAR BIOLOGY

The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae

(2020) Natalia I Reim et al.   NUCLEIC ACIDS RESEARCH

A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation

(2019) Rajaraman Gopalakrishnan et al.   NUCLEIC ACIDS RESEARCH

In vitro analysis of RNA polymerase II elongation complex dynamics

(2019) Yoo Jin Joo et al.   GENES & DEVELOPMENT

Selective kinase inhibition shows that Bur1 (Cdk9) phosphorylates the Rpb1 linker in vivo

(2019) Yujin Chun et al.   MOLECULAR AND CELLULAR BIOLOGY

The nucleosome acidic patch directly interacts with subunits of the Paf1 and FACT complexes and controls chromatin architecture in vivo

(2019) Christine E Cucinotta et al.   NUCLEIC ACIDS RESEARCH

Paf1C regulates RNA polymerase II progression by modulating elongation rate

(2019) Liming Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides

(2019) Zhen-Lin Chen et al.   Nature Communications

Histone Recycling by FACT and Spt6 during Transcription Prevents the Scrambling of Histone Modifications

(2019) Célia Jeronimo et al.   Cell Reports

Activation and regulation of H2B-Ubiquitin-dependent histone methyltransferases

(2019) Evan J Worden et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Transcription through the nucleosome

(2019) Tomoya Kujirai et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Born to run: control of transcription elongation by RNA polymerase II

(2018) Fei Xavier Chen et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structure of activated transcription complex Pol II–DSIF–PAF–SPT6

(2018) Seychelle M. Vos et al.   NATURE

Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology

(2018) Pujuan Deng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spt6 Is Required for the Fidelity of Promoter Selection

(2018) Stephen M. Doris et al.   MOLECULAR CELL

Paf1 Has Distinct Roles in Transcription Elongation and Differential Transcript Fate

(2017) Harry Fischl et al.   MOLECULAR CELL

The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain

(2017) Kevin M. Harlen et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Histone chaperone networks shaping chromatin function

(2017) Colin M. Hammond et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

PAF1 regulation of promoter-proximal pause release via enhancer activation

(2017) Fei Xavier Chen et al.   SCIENCE

Crystal structure of the N-terminal domain of human CDC73 and its implications for the hyperparathyroidism-jaw tumor (HPT-JT) syndrome

(2017) Wei Sun et al.   Scientific Reports

A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription

(2017) Matthew A Sdano et al.   eLife

Structure and Function of the Nuclear Pore Complex Cytoplasmic mRNA Export Platform

(2016) Javier Fernandez-Martinez et al.   CELL

The Histone Modification Domain of Paf1 Complex Subunit Rtf1 Directly Stimulates H2B Ubiquitylation through an Interaction with Rad6

(2016) S. Branden Van Oss et al.   MOLECULAR CELL

Direct Analysis of Phosphorylation Sites on the Rpb1 C-Terminal Domain of RNA Polymerase II

(2016) Hyunsuk Suh et al.   MOLECULAR CELL

deepTools2: a next generation web server for deep-sequencing data analysis

(2016) Fidel Ramírez et al.   NUCLEIC ACIDS RESEARCH

PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation

(2016) Yan Yang et al.   PLoS Genetics

PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II

(2015) Fei Xavier Chen et al.   CELL

The Abundant Histone Chaperones Spt6 and FACT Collaborate to Assemble, Inspect, and Maintain Chromatin Structure in Saccharomyces cerevisiae

(2015) L. McCullough et al.   GENETICS

Characterization of the Human Transcription Elongation Factor Rtf1: Evidence for Nonoverlapping Functions of Rtf1 and the Paf1 Complex

(2015) Qing-Fu Cao et al.   MOLECULAR AND CELLULAR BIOLOGY

A strategy for dissecting the architectures of native macromolecular assemblies

(2015) Yi Shi et al.   NATURE METHODS

The I-TASSER Suite: protein structure and function prediction

(2015) Jianyi Yang et al.   NATURE METHODS

RNA polymerase II-associated factor 1 regulates the release and phosphorylation of paused RNA polymerase II

(2015) M. Yu et al.   SCIENCE

The Nucleosome Acidic Patch Regulates the H2B K123 Monoubiquitylation Cascade and Transcription Elongation in Saccharomyces cerevisiae

(2015) Christine E. Cucinotta et al.   PLoS Genetics

Structural Characterization by Cross-linking Reveals the Detailed Architecture of a Coatomer-related Heptameric Module from the Nuclear Pore Complex

(2014) Yi Shi et al.   MOLECULAR & CELLULAR PROTEOMICS

deepTools: a flexible platform for exploring deep-sequencing data

(2014) Fidel Ramírez et al.   NUCLEIC ACIDS RESEARCH

The Recruitment of theSaccharomyces cerevisiaePaf1 Complex to Active Genes Requires a Domain of Rtf1 That Directly Interacts with the Spt4-Spt5 Complex

(2013) Manasi K. Mayekar et al.   MOLECULAR AND CELLULAR BIOLOGY

Spt6 Regulates Intragenic and Antisense Transcription, Nucleosome Positioning, and Histone Modifications Genome-Wide in Fission Yeast

(2013) C. M. DeGennaro et al.   MOLECULAR AND CELLULAR BIOLOGY

Structural basis for Spt5-mediated recruitment of the Paf1 complex to chromatin

(2013) A. D. Wier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3

(2013) Hiroaki Kato et al.   Scientific Reports

Transcription-associated histone modifications and cryptic transcription

(2012) Michaela Smolle et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration

(2012) H. Thorvaldsdottir et al.   BRIEFINGS IN BIOINFORMATICS

Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex

(2012) Hongfang Qiu et al.   EMBO JOURNAL

Cdc73 Subunit of Paf1 Complex Contains C-terminal Ras-like Domain That Promotes Association of Paf1 Complex with Chromatin

(2012) Christopher G. Amrich et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

Small region of Rtf1 protein can substitute for complete Paf1 complex in facilitating global histone H2B ubiquitylation in yeast

(2012) A. S. Piro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Putting the Pieces Together: Integrative Modeling Platform Software for Structure Determination of Macromolecular Assemblies

(2012) Daniel Russel et al.   PLOS BIOLOGY

Splitting the task: Ubp8 and Ubp10 deubiquitinate different cellular pools of H2BK123

(2011) J. M. Schulze et al.   GENES & DEVELOPMENT

Solution Structure of Tandem SH2 Domains from Spt6 Protein and Their Binding to the Phosphorylated RNA Polymerase II C-terminal Domain

(2011) Jianping Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structures of the S. cerevisiae Spt6 Core and C-Terminal Tandem SH2 Domain

(2011) Devin Close et al.   JOURNAL OF MOLECULAR BIOLOGY

The Human PAF1 Complex Acts in Chromatin Transcription Elongation Both Independently and Cooperatively with SII/TFIIS

(2010) Jaehoon Kim et al.   CELL

The structure of an Iws1/Spt6 complex reveals an interaction domain conserved in TFIIS, Elongin A and Med26

(2010) Marie-Laure Diebold et al.   EMBO JOURNAL

Noncanonical Tandem SH2 Enables Interaction of Elongation Factor Spt6 with RNA Polymerase II

(2010) Marie-Laure Diebold et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD)

(2010) Mai Sun et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and Biological Importance of the Spn1-Spt6 Interaction, and Its Regulatory Role in Nucleosome Binding

(2010) Seth M. McDonald et al.   MOLECULAR CELL

I-TASSER: a unified platform for automated protein structure and function prediction

(2010) Ambrish Roy et al.   Nature Protocols

Uniform transitions of the general RNA polymerase II transcription complex

(2010) Andreas Mayer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Sequence Alignment/Map format and SAMtools

(2009) H. Li et al.   BIOINFORMATICS

Spt6 enhances the elongation rate of RNA polymerase II in vivo

(2009) M Behfar Ardehali et al.   EMBO JOURNAL

Structure and in Vivo Requirement of the Yeast Spt6 SH2 Domain

(2009) Stefan Dengl et al.   JOURNAL OF MOLECULAR BIOLOGY

Phosphorylation of the Transcription Elongation Factor Spt5 by Yeast Bur1 Kinase Stimulates Recruitment of the PAF Complex

(2009) Y. Liu et al.   MOLECULAR AND CELLULAR BIOLOGY

An auxin-based degron system for the rapid depletion of proteins in nonplant cells

(2009) Kohei Nishimura et al.   NATURE METHODS

Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5

(2009) K. Zhou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

I-TASSER server for protein 3D structure prediction

(2008) Yang Zhang   BMC BIOINFORMATICS

Roles for Ctk1 and Spt6 in Regulating the Different Methylation States of Histone H3 Lysine 36

(2008) M. L. Youdell et al.   MOLECULAR AND CELLULAR BIOLOGY