Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT

Spt5 histone binding activity preserves chromatin during transcription by RNA polymerase II

(2022) Cecile Evrin et al.   EMBO JOURNAL

Structural basis of nucleosome retention during transcription elongation

(2022) Martin Filipovski et al.   SCIENCE

Nucleosome Positioning and Spacing: From Mechanism to Function

(2021) Ashish Kumar Singh et al.   JOURNAL OF MOLECULAR BIOLOGY

A high-resolution protein architecture of the budding yeast genome

(2021) Matthew J. Rossi et al.   NATURE

Essential histone chaperones collaborate to regulate transcription and chromatin integrity

(2021) Olga Viktorovskaya et al.   GENES & DEVELOPMENT

The Paf1 Complex: A Keystone of Nuclear Regulation Operating at the Interface of Transcription and Chromatin

(2021) Alex M. Francette et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural basis of nucleosome transcription mediated by Chd1 and FACT

(2021) Lucas Farnung et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CDK9: A Comprehensive Review of Its Biology, and Its Role as a Potential Target for Anti-Cancer Agents

(2021) Abel Tesfaye Anshabo et al.   Frontiers in Oncology

RNA polymerase II speed: a key player in controlling and adapting transcriptome composition

(2021) Lisa Muniz et al.   EMBO JOURNAL

Two distinct mechanisms of RNA polymerase II elongation stimulation in vivo

(2021) Kristina Žumer et al.   MOLECULAR CELL

Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination

(2021) Ashwin Narain et al.   MOLECULAR CELL

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

A ubiquitous disordered protein interaction module orchestrates transcription elongation

(2021) Katerina Cermakova et al.   SCIENCE

Structure of complete Pol II–DSIF–PAF–SPT6 transcription complex reveals RTF1 allosteric activation

(2020) Seychelle M. Vos et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanisms of Transcription Elongation Factor DSIF (Spt4–Spt5)

(2020) Tim-Michael Decker   JOURNAL OF MOLECULAR BIOLOGY

The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae

(2020) Natalia I Reim et al.   NUCLEIC ACIDS RESEARCH

The role of FACT in managing chromatin: disruption, assembly, or repair?

(2020) Tim Formosa et al.   NUCLEIC ACIDS RESEARCH

Structural insight into nucleosome transcription by RNA polymerase II with elongation factors

(2019) Haruhiko Ehara et al.   SCIENCE

The nucleosome acidic patch directly interacts with subunits of the Paf1 and FACT complexes and controls chromatin architecture in vivo

(2019) Christine E Cucinotta et al.   NUCLEIC ACIDS RESEARCH

Structural visualization of key steps in nucleosome reorganization by human FACT

(2019) Kouta Mayanagi et al.   Scientific Reports

Histone Recycling by FACT and Spt6 during Transcription Prevents the Scrambling of Histone Modifications

(2019) Célia Jeronimo et al.   Cell Reports

Organization and regulation of gene transcription

(2019) Patrick Cramer   NATURE

FACT caught in the act of manipulating the nucleosome

(2019) Yang Liu et al.   NATURE

Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs

(2019) Tristan Bepler et al.   NATURE METHODS

Transcription through the nucleosome

(2019) Tomoya Kujirai et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Casein kinase 2 mediated phosphorylation of Spt6 modulates histone dynamics and regulates spurious transcription

(2018) Emmanuelle Gouot et al.   NUCLEIC ACIDS RESEARCH

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

(2018) Takanori Nakane et al.   eLife

ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps

(2018) Tristan Ian Croll   Acta Crystallographica Section D-Structural Biology

Structure of activated transcription complex Pol II–DSIF–PAF–SPT6

(2018) Seychelle M. Vos et al.   NATURE

Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology

(2018) Pujuan Deng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Paf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation

(2018) Ying Xie et al.   Nature Communications

Spt6 Is Required for the Fidelity of Promoter Selection

(2018) Stephen M. Doris et al.   MOLECULAR CELL

Structural basis of the nucleosome transition during RNA polymerase II passage

(2018) Tomoya Kujirai et al.   SCIENCE

Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction

(2018) Raghuvar Dronamraju et al.   Cell Reports

Residues in the Nucleosome Acidic Patch Regulate Histone Occupancy and Are Important for FACT Binding in Saccharomyces cerevisiae

(2017) Amelia J. Hodges et al.   GENETICS

Transcription and Remodeling Produce Asymmetrically Unwrapped Nucleosomal Intermediates

(2017) Srinivas Ramachandran et al.   MOLECULAR CELL

The elongation factor Spn1 is a multi-functional chromatin binding protein

(2017) Sha Li et al.   NUCLEIC ACIDS RESEARCH

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Structure of the complete elongation complex of RNA polymerase II with basal factors

(2017) Haruhiko Ehara et al.   SCIENCE

Residues in the Nucleosome Acidic Patch Regulate Histone Occupancy and Are Important for FACT Binding in Saccharomyces cerevisiae

(2017) Amelia J. Hodges et al.   GENETICS

Integrated molecular mechanism directing nucleosome reorganization by human FACT

(2016) Yasuo Tsunaka et al.   GENES & DEVELOPMENT

Quantitative Analysis of Dynamic Protein Interactions during Transcription Reveals a Role for Casein Kinase II in Polymerase-associated Factor (PAF) Complex Phosphorylation and Regulation of Histone H2B Monoubiquitylation

(2016) Lynn Glowczewski Bedard et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Near-atomic resolution visualization of human transcription promoter opening

(2016) Yuan He et al.   NATURE

Transcription initiation complex structures elucidate DNA opening

(2016) C. Plaschka et al.   NATURE

The Abundant Histone Chaperones Spt6 and FACT Collaborate to Assemble, Inspect, and Maintain Chromatin Structure in Saccharomyces cerevisiae

(2015) L. McCullough et al.   GENETICS

FACT Disrupts Nucleosome Structure by Binding H2A-H2B with Conserved Peptide Motifs

(2015) David J. Kemble et al.   MOLECULAR CELL

Molecular structures of unbound and transcribing RNA polymerase III

(2015) Niklas A. Hoffmann et al.   NATURE

Histone exchange, chromatin structure and the regulation of transcription

(2015) Swaminathan Venkatesh et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural analysis of nucleosomal barrier to transcription

(2015) Daria A. Gaykalova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation

(2014) Harihar Basnet et al.   NATURE

Control of Transcriptional Elongation

(2013) Hojoong Kwak et al.   Annual Review of Genetics

RNA polymerase I structure and transcription regulation

(2013) Christoph Engel et al.   NATURE

Crystal structure of the 14-subunit RNA polymerase I

(2013) Carlos Fernández-Tornero et al.   NATURE

Structural insights into Paf1 complex assembly and histone binding

(2013) Xinlei Chu et al.   NUCLEIC ACIDS RESEARCH

Structural basis for Spt5-mediated recruitment of the Paf1 complex to chromatin

(2013) A. D. Wier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Histone chaperone FACT action during transcription through chromatin by RNA polymerase II

(2013) F.-K. Hsieh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex

(2012) Hongfang Qiu et al.   EMBO JOURNAL

Cdc73 Subunit of Paf1 Complex Contains C-terminal Ras-like Domain That Promotes Association of Paf1 Complex with Chromatin

(2012) Christopher G. Amrich et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structures of the S. cerevisiae Spt6 Core and C-Terminal Tandem SH2 Domain

(2011) Devin Close et al.   JOURNAL OF MOLECULAR BIOLOGY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The structure of an Iws1/Spt6 complex reveals an interaction domain conserved in TFIIS, Elongin A and Med26

(2010) Marie-Laure Diebold et al.   EMBO JOURNAL

The Transcription Factor Spn1 Regulates Gene Expression via a Highly Conserved Novel Structural Motif

(2010) Venugopal Pujari et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystal Structures of Nucleosome Core Particles Containing the ‘601’ Strong Positioning Sequence

(2010) Dileep Vasudevan et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure and Biological Importance of the Spn1-Spt6 Interaction, and Its Regulatory Role in Nucleosome Binding

(2010) Seth M. McDonald et al.   MOLECULAR CELL

Spt6 enhances the elongation rate of RNA polymerase II in vivo

(2009) M Behfar Ardehali et al.   EMBO JOURNAL

Histone Chaperone Spt16 Promotes Redeposition of the Original H3-H4 Histones Evicted by Elongating RNA Polymerase

(2009) Adil Jamai et al.   MOLECULAR CELL

Mechanism of chromatin remodeling and recovery during passage of RNA polymerase II

(2009) Olga I Kulaeva et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY