Mass Spectrometry of RNA-Binding Proteins during Liquid–Liquid Phase Separation Reveals Distinct Assembly Mechanisms and Droplet Architectures

In vivo liquid–liquid phase separation protects amyloidogenic and aggregation‐prone peptides during overexpression in Escherichia coli

(2022) Bartosz Gabryelczyk et al.   PROTEIN SCIENCE

Sequence grammar underlying the unfolding and phase separation of globular proteins

(2022) Kiersten M. Ruff et al.   MOLECULAR CELL

Using mass spectrometry‐based methods to understand amyloid formation and inhibition of alpha‐synuclein and amyloid beta

(2022) Wesley J. Wagner et al.   MASS SPECTROMETRY REVIEWS

FUS and TDP-43 Phases in Health and Disease

(2021) Bede Portz et al.   TRENDS IN BIOCHEMICAL SCIENCES

A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions

(2021) Joris Van Lindt et al.   Communications Biology

NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation

(2021) Leonidas Emmanouilidis et al.   Nature Chemical Biology

Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions

(2021) Georg Krainer et al.   Nature Communications

Cryo-EM structure of amyloid fibrils formed by the entire low complexity domain of TDP-43

(2021) Qiuye Li et al.   Nature Communications

Predicting protein condensate formation using machine learning

(2021) Guido van Mierlo et al.   Cell Reports

TDP-43 condensation properties specify its RNA-binding and regulatory repertoire

(2021) Martina Hallegger et al.   CELL

Droplet and fibril formation of the functional amyloid Orb2

(2021) Kidist Ashami et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Generic nature of the condensed states of proteins

(2021) Monika Fuxreiter et al.   NATURE CELL BIOLOGY

Vectorial channeling as a mechanism for translational control by functional prions and condensates

(2021) Xinyu Gu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The (un)structural biology of biomolecular liquid-liquid phase separation using NMR spectroscopy

(2020) Anastasia C. Murthy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila

(2020) Ruben Hervas et al.   SCIENCE

Predicting the shapes of protein complexes through collision cross section measurements and database searches

(2020) Michael Landreh et al.   ANALYTICAL CHEMISTRY

Purification and Structural Characterization of Aggregation-Prone Human TDP-43 Involved in Neurodegenerative Diseases

(2020) Gareth S.A. Wright et al.   iScience

Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration

(2020) Veronica H Ryan et al.   EMBO JOURNAL

Widespread occurrence of the droplet state of proteins in the human proteome

(2020) Maarten Hardenberg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ion Mobility Mass Spectrometry Uncovers the Impact of the Patterning of Oppositely Charged Residues on the Conformational Distributions of Intrinsically Disordered Proteins

(2019) Rebecca Beveridge et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Cryo-EM structures of four polymorphic TDP-43 amyloid cores

(2019) Qin Cao et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CPEB3 inhibits translation of mRNA targets by localizing them to P bodies

(2019) Lenzie Ford et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing

(2018) Ailin Wang et al.   EMBO JOURNAL

A User’s Guide for Phase Separation Assays with Purified Proteins

(2018) Simon Alberti et al.   JOURNAL OF MOLECULAR BIOLOGY

Atomic structures of TDP-43 LCD segments and insights into reversible or pathogenic aggregation

(2018) Elizabeth L. Guenther et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Atomic structures of TDP-43 LCD segments and insights into reversible or pathogenic aggregation

(2018) Elizabeth L. Guenther et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Poly(ADP-ribose) Engages the TDP-43 Nuclear-Localization Sequence to Regulate Granulo-Filamentous Aggregation

(2018) Leeanne McGurk et al.   BIOCHEMISTRY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Efficient protein production inspired by how spiders make silk

(2017) Nina Kronqvist et al.   Nature Communications

Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation

(2017) Tariq Afroz et al.   Nature Communications

A Putative Biochemical Engram of Long-Term Memory

(2016) Liying Li et al.   CURRENT BIOLOGY

The Role of Functional Prion-Like Proteins in the Persistence of Memory

(2016) Kausik Si et al.   Cold Spring Harbor Perspectives in Biology

Ensemble Methods Enable a New Definition for the Solution to Gas-Phase Transfer of Intrinsically Disordered Proteins

(2015) Antoni J. Borysik et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

The CPEB3 Protein Is a Functional Prion that Interacts with the Actin Cytoskeleton

(2015) Joseph S. Stephan et al.   Cell Reports

TDP-43 is a key player in the clinical features associated with Alzheimer’s disease

(2014) Keith A. Josephs et al.   ACTA NEUROPATHOLOGICA

Characterization of prion-like conformational changes of the neuronal isoform of Aplysia CPEB

(2013) Bindu L Raveendra et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The tip of the iceberg: RNA-binding proteins with prion-like domains in neurodegenerative disease

(2012) Oliver D. King et al.   BRAIN RESEARCH

AWSEM-MD: Protein Structure Prediction Using Coarse-Grained Physical Potentials and Bioinformatically Based Local Structure Biasing

(2012) Aram Davtyan et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Aplysia CPEB Can Form Prion-like Multimers in Sensory Neurons that Contribute to Long-Term Facilitation

(2010) Kausik Si et al.   CELL

Direct measurement of islet amyloid polypeptide fibrillogenesis by mass spectrometry

(2010) Jennifer L. Larson et al.   PROTEIN SCIENCE

Collisional activation of protein complexes: Picking up the pieces

(2008) Justin L. P. Benesch   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY