The (un)structural biology of biomolecular liquid-liquid phase separation using NMR spectroscopy

Phosphoregulated FMRP phase separation models activity-dependent translation through bidirectional control of mRNA granule formation

(2019) Brian Tsang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Heterochromatin protein HP1α gelation dynamics revealed by solid-state NMR spectroscopy

(2019) Bryce E Ackermann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Phase separation of RNA-binding proteins in physiology and disease: An introduction to the JBC Reviews thematic series

(2019) James Shorter   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain

(2019) Anastasia C. Murthy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Lysine/RNA-interactions drive and regulate biomolecular condensation

(2019) Tina Ukmar-Godec et al.   Nature Communications

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

First-generation predictors of biological protein phase separation

(2019) Robert M Vernon et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

(2019) David T. McSwiggen et al.   GENES & DEVELOPMENT

Physiological, Pathological, and Targetable Membraneless Organelles in Neurons

(2019) Veronica H. Ryan et al.   TRENDS IN NEUROSCIENCES

Atomic-level insight into mRNA processing bodies by combining solid and solution-state NMR spectroscopy

(2019) Reinier Damman et al.   Nature Communications

NPM1 exhibits structural and dynamic heterogeneity upon phase separation with the p14ARF tumor suppressor

(2019) Eric Gibbs et al.   JOURNAL OF MAGNETIC RESONANCE

Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites

(2018) Takuya Yoshizawa et al.   CELL

Role of Liquid–Liquid Phase Separation in Assembly of Elastin and Other Extracellular Matrix Proteins

(2018) Lisa D. Muiznieks et al.   JOURNAL OF MOLECULAR BIOLOGY

Probing Conformational Exchange in Weakly Interacting, Slowly Exchanging Protein Systems via Off-Resonance R1ρ Experiments: Application to Studies of Protein Phase Separation

(2018) Tairan Yuwen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions

(2018) Thuy P. Dao et al.   MOLECULAR CELL

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation

(2018) Veronica H. Ryan et al.   MOLECULAR CELL

The SH3 domain of Fyn kinase interacts with and induces liquid–liquid phase separation of the low-complexity domain of hnRNPA2

(2018) Joshua Amaya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Highly disordered histone H1−DNA model complexes and their condensates

(2018) Abigail L. Turner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct observation of structure and dynamics during phase separation of an elastomeric protein

(2017) Sean E. Reichheld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A molecular engineering toolbox for the structural biologist

(2017) Galia T. Debelouchina et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Amyloid-like Self-Assembly of a Cellular Compartment

(2016) Elvan Boke et al.   CELL

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA

(2016) Diana M Mitrea et al.   eLife

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Visualizing transient dark states by NMR spectroscopy

(2015) Nicholas J. Anthis et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Phosphorylation-Regulated Binding of RNA Polymerase II to Fibrous Polymers of Low-Complexity Domains

(2013) Ilmin Kwon et al.   CELL

Describing intrinsically disordered proteins at atomic resolution by NMR

(2013) Malene Ringkjøbing Jensen et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Probing the transient dark state of substrate binding to GroEL by relaxation-based solution NMR

(2013) D. S. Libich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Determination of Secondary Structure Populations in Disordered States of Proteins Using Nuclear Magnetic Resonance Chemical Shifts

(2012) Carlo Camilloni et al.   BIOCHEMISTRY

Characterization of disordered proteins with ENSEMBLE

(2012) Mickaël Krzeminski et al.   BIOINFORMATICS

The tip of the iceberg: RNA-binding proteins with prion-like domains in neurodegenerative disease

(2012) Oliver D. King et al.   BRAIN RESEARCH

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Atomic-resolution dynamics on the surface of amyloid-β protofibrils probed by solution NMR

(2011) Nicolas L. Fawzi et al.   NATURE

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Protein Phase Behavior in Aqueous Solutions: Crystallization, Liquid-Liquid Phase Separation, Gels, and Aggregates

(2007) André C. Dumetz et al.   BIOPHYSICAL JOURNAL