Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

(2023) Zhen Chen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM structure of an active central apparatus

(2022) Long Han et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Ciliary central apparatus structure reveals mechanisms of microtubule patterning

(2022) Miao Gui et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules

(2022) Miao Gui et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The multi‐scale architecture of mammalian sperm flagella and implications for ciliary motility

(2021) Miguel Ricardo Leung et al.   EMBO JOURNAL

The Fertilization Enigma: How Sperm and Egg Fuse

(2021) Victoria E. Deneke et al.   Annual Review of Cell and Developmental Biology

Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse

(2021) Jintao Zhang et al.   HUMAN MOLECULAR GENETICS

3D variability analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM

(2021) Ali Punjani et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-EM structure of cortical microtubules from human parasite Toxoplasma gondii identifies their microtubule inner proteins

(2021) Xiangli Wang et al.   Nature Communications

Essential Role of CFAP53 in Sperm Flagellum Biogenesis

(2021) Bingbing Wu et al.   Frontiers in Cell and Developmental Biology

De novo identification of mammalian ciliary motility proteins using cryo-EM

(2021) Miao Gui et al.   CELL

AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models

(2021) Mihaly Varadi et al.   NUCLEIC ACIDS RESEARCH

The genetic architecture of morphological abnormalities of the sperm tail

(2020) Aminata Touré et al.   HUMAN GENETICS

DPHL: A DIA Pan-human Protein Mass Spectrometry Library for Robust Biomarker Discovery

(2020) Tiansheng Zhu et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

UCSF ChimeraX : Structure visualization for researchers, educators, and developers

(2020) Eric F. Pettersen et al.   PROTEIN SCIENCE

Structures of radial spokes and associated complexes important for ciliary motility

(2020) Miao Gui et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

DeepTracer for fast de novo cryo-EM protein structure modeling and special studies on CoV-related complexes

(2020) Jonas Pfab et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Inner lumen proteins stabilize doublet microtubules in cilia and flagella

(2019) Mikito Owa et al.   Nature Communications

Regulation of Dual-Specificity Phosphatase (DUSP) Ubiquitination and Protein Stability

(2019) Hsueh-Fen Chen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Mutations in DNAH17, Encoding a Sperm-Specific Axonemal Outer Dynein Arm Heavy Chain, Cause Isolated Male Infertility Due to Asthenozoospermia

(2019) Marjorie Whitfield et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Structure of the Decorated Ciliary Doublet Microtubule

(2019) Meisheng Ma et al.   CELL

Sperm defects in primary ciliary dyskinesia and related causes of male infertility

(2019) Anu Sironen et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Genetics of male infertility

(2018) Csilla Krausz et al.   Nature Reviews Urology

SWISS-MODEL: homology modelling of protein structures and complexes

(2018) Andrew Waterhouse et al.   NUCLEIC ACIDS RESEARCH

Asymmetric distribution and spatial switching of dynein activity generates ciliary motility

(2018) Jianfeng Lin et al.   SCIENCE

The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice

(2018) Manan Khan et al.   Scientific Reports

Real-space refinement in PHENIX for cryo-EM and crystallography

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

Homozygous loss-of-function mutations in MNS1 cause laterality defects and likely male infertility

(2018) Asaf Ta-Shma et al.   PLoS Genetics

Effects of stably incorporated iron on protein phosphatase-1 structure and activity

(2018) Francesca Salvi et al.   FEBS LETTERS

Comprehensive identification of peptides in tandem mass spectra using an efficient open search engine

(2018) Hao Chi et al.   NATURE BIOTECHNOLOGY

Quantitative Proteomic Analysis of Human Airway Cilia Identifies Previously Uncharacterized Proteins of High Abundance

(2017) Kevin Blackburn et al.   JOURNAL OF PROTEOME RESEARCH

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Axoneme Structure from Motile Cilia

(2016) Takashi Ishikawa   Cold Spring Harbor Perspectives in Biology

Radial Spokes—A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella

(2016) Xiaoyan Zhu et al.   Cold Spring Harbor Perspectives in Biology

Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskinesia and Disrupt the Nexin-Dynein Regulatory Complex

(2015) Heike Olbrich et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Teratozoospermia: spotlight on the main genetic actors in the human

(2015) C. Coutton et al.   HUMAN REPRODUCTION UPDATE

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

(2015) Alan Brown et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Branchial Cilia and Sperm Flagella Recruit Distinct Axonemal Components

(2015) Alu Konno et al.   PLoS One

High-resolution crystal structure of the catalytic domain of human dual-specificity phosphatase 26

(2013) Eun-Young Won et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Mutations in DNAH1, which Encodes an Inner Arm Heavy Chain Dynein, Lead to Male Infertility from Multiple Morphological Abnormalities of the Sperm Flagella

(2013) Mariem Ben Khelifa et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Human Sperm Tail Proteome Suggests New Endogenous Metabolic Pathways

(2012) Alexandra Amaral et al.   MOLECULAR & CELLULAR PROTEOMICS

MNS1 Is Essential for Spermiogenesis and Motile Ciliary Functions in Mice

(2012) Jian Zhou et al.   PLoS Genetics

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

(2011) F. Sievers et al.   Molecular Systems Biology

Skyline: an open source document editor for creating and analyzing targeted proteomics experiments

(2010) Brendan MacLean et al.   BIOINFORMATICS

Characterization of a Novel Tektin Member, TEKT5, in Mouse Sperm

(2010) W. Cao et al.   JOURNAL OF ANDROLOGY

MutationTaster evaluates disease-causing potential of sequence alterations

(2010) Jana Marie Schwarz et al.   NATURE METHODS

World Health Organization reference values for human semen characteristics*‡

(2009) Trevor G. Cooper et al.   HUMAN REPRODUCTION UPDATE

Tektin 3 is required for progressive sperm motility in mice

(2008) Angshumoy Roy et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Swimming with protists: perception, motility and flagellum assembly

(2008) Michael L. Ginger et al.   NATURE REVIEWS MICROBIOLOGY

Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa

(2007) Emi Murayama et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT