Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineering

Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coli BL21 (DE3)

(2011) Hendrik Waegeman et al.   BMC MICROBIOLOGY

Engineering of Therapeutic Proteins Production in Escherichia coli

(2011) Mariusz Kamionka   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

(2011) Thorben Dammeyer et al.   Microbial Cell Factories

Effects of co-expression of molecular chaperones on heterologous soluble expression of the cold-active lipase Lip-948

(2011) Cui Shuo-shuo et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of Secretory and Extracellular N-Linked Glycoproteins inEscherichia coli

(2010) Adam C. Fisher et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Fed-Batch Production of a Thermophilic 2-Deoxyribose-5-Phosphate Aldolase (DERA) in Escherichia coli by Exponential Feeding Strategy Control

(2010) Xiaolin Pei et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Production of recombinant proteins and metabolites in yeasts

(2010) Danilo Porro et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Transport and proofreading of proteins by the twin-arginine translocation (Tat) system in bacteria

(2010) Colin Robinson et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Technical refolding of proteins: Do we have freedom to operate?

(2010) Maria K. Eiberle et al.   Biotechnology Journal

N-Linked glycoengineering for human therapeutic proteins in bacteria

(2010) Jagroop Pandhal et al.   BIOTECHNOLOGY LETTERS

Chymosin and other milk coagulants: sources and biotechnological interventions

(2010) A. Kumar et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Extracellular production of recombinant proteins using bacterial autotransporters

(2010) Wouter SP Jong et al.   CURRENT OPINION IN BIOTECHNOLOGY

Post-translational modifications of protein biopharmaceuticals

(2010) Gary Walsh   DRUG DISCOVERY TODAY

Enhancing the protein production levels in Escherichia coli with a strong promoter

(2010) Hanna Tegel et al.   FEBS Journal

Transient metabolic modeling of Escherichia coli MG1655 and MG1655 ΔackA-pta, ΔpoxB Δpppc ppc-p37 for recombinant β-galactosidase production

(2010) Marjan De Mey et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Side effects of chaperone gene co-expression in recombinant protein production

(2010) Mónica Martínez-Alonso et al.   Microbial Cell Factories

Production of Hepatopoietin Cn in High-Cell-Density Cultures of Recombinant Escherichia coli and Detection of its Antioxygen Activity

(2010) Yang Liu et al.   MOLECULAR BIOTECHNOLOGY

Protein glycosylation in bacteria: sweeter than ever

(2010) Harald Nothaft et al.   NATURE REVIEWS MICROBIOLOGY

High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli

(2010) Danping Zhang et al.   PROCESS BIOCHEMISTRY

Designing and engineering evolutionary robust genetic circuits

(2010) Sean C Sleight et al.   Journal of Biological Engineering

Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli

(2009) N. Narayanan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhancement of Human γ-Interferon Production in Recombinant E. coli Using Batch Cultivation

(2009) Valiollah Babaeipour et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Production of pig liver esterase in batch fermentation of E. coli Origami

(2009) Elke Brüsehaber et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancement of human granulocyte-colony stimulating factor production in recombinant E. coli using batch cultivation

(2009) Valiollah Babaeipour et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Enhanced production of recombinant streptokinase in Escherichia coli using fed-batch culture

(2009) Deepika Goyal et al.   BIORESOURCE TECHNOLOGY

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Extracellular recombinant protein production from Escherichia coli

(2009) Ye Ni et al.   BIOTECHNOLOGY LETTERS

Bacterial artificial chromosomes improve recombinant protein production in mammalian cells

(2009) Leander Blaas et al.   BMC BIOTECHNOLOGY

Protein secretion systems in bacterial-host associations, and their description in the Gene Ontology

(2009) Tsai-Tien Tseng et al.   BMC MICROBIOLOGY

Expression systems for therapeutic glycoprotein production

(2009) Yves Durocher et al.   CURRENT OPINION IN BIOTECHNOLOGY

Optimal and consistent protein glycosylation in mammalian cell culture

(2009) P. Hossler et al.   GLYCOBIOLOGY

Genome Sequences of Escherichia coli B strains REL606 and BL21(DE3)

(2009) Haeyoung Jeong et al.   JOURNAL OF MOLECULAR BIOLOGY

Multiple-copy-gene integration on chromosome of Escherichia coli for beta-galactosidase production

(2009) Hsing-Ta Chen et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Microbial factories for recombinant pharmaceuticals

(2009) Neus Ferrer-Miralles et al.   Microbial Cell Factories

Use of folding modulators to improve heterologous protein production in Escherichia coli

(2009) Olga Kolaj et al.   Microbial Cell Factories

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

(2009) Ario de Marco   Microbial Cell Factories

Sales of biologics to show robust growth through to 2013

(2009) Michael Goodman   NATURE REVIEWS DRUG DISCOVERY

Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3)

(2009) Hanna Tegel et al.   PROTEIN EXPRESSION AND PURIFICATION

Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones

(2009) Hiroyuki Sonoda et al.   PROTEIN EXPRESSION AND PURIFICATION

Principles of general and regulatory proteolysis by AAA+ proteases in Escherichia coli

(2009) Ronny Schmidt et al.   RESEARCH IN MICROBIOLOGY

Approaches for refining heterologous protein production in filamentous fungi

(2009) Ruchika Sharma et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Enhancement of Recombinant Human ADAM15 Disintegrin Domain Expression Level by Releasing the Rare Codons and Amino Acids Restriction

(2008) Jing Wu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture

(2008) Hisashi Semba et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Functional expression of porcine aminoacylase 1 in E. coli using a codon optimized synthetic gene and molecular chaperones

(2008) Rainer Wardenga et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Not just for Eukarya anymore: protein glycosylation in Bacteria and Archaea

(2008) Mehtap Abu-Qarn et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Large-Scale Production of Major House Dust Mite Allergen Der f 2 Mutant (C8/119S) in Escherichia coli

(2008) Satoshi Koyanagi et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Effects of glycosylation on the stability of protein pharmaceuticals

(2008) Ricardo J. Solá et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

(2008) Brigitte Gasser et al.   Microbial Cell Factories

The bacterial type VI secretion machine: yet another player for protein transport across membranes

(2008) A. Filloux et al.   MICROBIOLOGY-SGM

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

(2008) Nicola A. Burgess-Brown et al.   PROTEIN EXPRESSION AND PURIFICATION

Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane—Distinct translocases and mechanisms

(2007) Paolo Natale et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

High-Level Production of Bacillus subtilis Glycine Oxidase by Fed-Batch Cultivation of Recombinant Escherichia coli Rosetta (DE3)

(2007) Irene Martínez-Martínez et al.   BIOTECHNOLOGY PROGRESS

Reduction of acetate accumulation in Escherichia coli cultures for increased recombinant protein production

(2007) Matthew S. Wong et al.   METABOLIC ENGINEERING

Redistribution of Metabolic Fluxes in the Central Aerobic Metabolic Pathway of E. coli Mutant Strains with Deletion of the ackA-pta and poxB Pathways for the Synthesis of Isoamyl Acetate

(2005) Cheryl R. Dittrich et al.   BIOTECHNOLOGY PROGRESS