Thiolase: A Versatile Biocatalyst Employing Coenzyme A–Thioester Chemistry for Making and Breaking C–C Bonds

Engineering potassium activation into biosynthetic thiolase

(2021) Andrew C. Marshall et al.   BIOCHEMICAL JOURNAL

Substrate specificity and conformational flexibility properties of the Mycobacterium tuberculosis β-oxidation trifunctional enzyme

(2021) Subhadra Dalwani et al.   JOURNAL OF STRUCTURAL BIOLOGY

Inactive pseudoenzyme subunits in heterotetrameric BbsCD, a novel short‐chain alcohol dehydrogenase involved in anaerobic toluene degradation

(2021) Silke Horsten et al.   FEBS Journal

The 3-ketoacyl-CoA thiolase: an engineered enzyme for carbon chain elongation of chemical compounds

(2020) Lixia Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystal structure of human mitochondrial trifunctional protein, a fatty acid β-oxidation metabolon

(2019) Chuanwu Xia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mutation update on ACAT1 variants associated with mitochondrial acetoacetyl‐CoA thiolase (T2) deficiency

(2019) Elsayed Abdelkreem et al.   HUMAN MUTATION

Structural and Biochemical Studies of Substrate Selectivity in Ascaris suum Thiolases

(2018) Michael R. Blaisse et al.   BIOCHEMISTRY

The role of OleA His285 in orchestration of long-chain acyl-coenzyme A substrates

(2018) Matthew R. Jensen et al.   FEBS LETTERS

Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway

(2018) Christopher R. Mehrer et al.   METABOLIC ENGINEERING

Cryo-EM structure of human mitochondrial trifunctional protein

(2018) Kai Liang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of Aspergillus fumigatus Cytosolic Thiolase: Trapped Tetrahedral Reaction Intermediates and Activation by Monovalent Cations

(2018) Andrew C. Marshall et al.   ACS Catalysis

De novo biosynthesis of sterols and fatty acids in the Trypanosoma brucei procyclic form: Carbon source preferences and metabolic flux redistributions

(2018) Yoann Millerioux et al.   PLoS Pathogens

The peroxisomal zebrafish SCP2-thiolase (type-1) is a weak transient dimer as revealed by crystal structures and native mass spectrometry

(2018) Tiila-Riikka Kiema et al.   BIOCHEMICAL JOURNAL

Active Multienzyme Assemblies for Long-Chain Olefinic Hydrocarbon Biosynthesis

(2017) James K. Christenson et al.   JOURNAL OF BACTERIOLOGY

Nickel-Catalyzed N-Arylation Using N-Trimethylsilyl-carbazole

(2017) Yasunori Minami et al.   SYNLETT

Aminoacyl-tRNA synthetases and the evolution of coded peptide synthesis: the Thioester World

(2016) Hieronim Jakubowski   FEBS LETTERS

Acetyl-CoA and the regulation of metabolism: mechanisms and consequences

(2015) Lei Shi et al.   CURRENT OPINION IN CELL BIOLOGY

Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous β-ketoacyl thiolase from Ralstonia eutropha

(2015) Christopher D. Fage et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

FadA5 a Thiolase from Mycobacterium tuberculosis : A Steroid-Binding Pocket Reveals the Potential for Drug Development against Tuberculosis

(2015) Christin M. Schaefer et al.   STRUCTURE

Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum

(2015) Sangwoo Kim et al.   Nature Communications

Structures of yeast peroxisomal Δ3,Δ2-enoyl-CoA isomerase complexed with acyl-CoA substrate analogues: the importance of hydrogen-bond networks for the reactivity of the catalytic base and the oxyanion hole

(2015) Goodluck U. Onwukwe et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Phylogenetic relationships and classification of thiolases and thiolase-like proteins of Mycobacterium tuberculosis and Mycobacterium smegmatis

(2014) Padmanabhan Anbazhagan et al.   TUBERCULOSIS

The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities

(2014) Tiila-Riikka Kiema et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Crystal structures of SCP2-thiolases of Trypanosomatidae, human pathogens causing widespread tropical diseases: the importance for catalysis of the cysteine of the unique HDCF loop

(2013) Rajesh K. Harijan et al.   BIOCHEMICAL JOURNAL

Specificity in Transition State Binding: The Pauling Model Revisited

(2013) Tina L. Amyes et al.   BIOCHEMISTRY

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

(2013) Collin H. Martin et al.   Nature Communications

Water Networks in Fast Proton Transfer during Catalysis by Human Carbonic Anhydrase II

(2012) Rose Mikulski et al.   BIOCHEMISTRY

Stereoelectronic Requirements for Optimal Hydrogen-Bond-Catalyzed Enolization

(2011) Imre Pápai et al.   CHEMISTRY-A EUROPEAN JOURNAL

Hydrogen-bond stabilization in oxyanion holes: grand jeté to three dimensions

(2011) Luis Simón et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Peroxisomal Plant 3-Ketoacyl-CoA Thiolase Structure and Activity Are Regulated by a Sensitive Redox Switch

(2010) Valerie E. Pye et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

On Catalytic Preorganization in Oxyanion Holes: Highlighting the Problems with the Gas-Phase Modeling of Oxyanion Holes and Illustrating the Need for Complete Enzyme Models

(2010) Shina C. L. Kamerlin et al.   JOURNAL OF ORGANIC CHEMISTRY

The Thiolase Reaction Mechanism: The Importance of Asn316 and His348 for Stabilizing the Enolate Intermediate of the Claisen Condensation

(2009) Gitte Meriläinen et al.   BIOCHEMISTRY

The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme

(2008) Gitte Meriläinen et al.   FEBS Journal

Divergent evolution of the thiolase superfamily and chalcone synthase family

(2008) C JIANG et al.   MOLECULAR PHYLOGENETICS AND EVOLUTION