The 3-ketoacyl-CoA thiolase: an engineered enzyme for carbon chain elongation of chemical compounds

Dynamic balancing of intestinal short-chain fatty acids: The crucial role of bacterial metabolism

(2020) Youqiang Xu et al.   TRENDS IN FOOD SCIENCE & TECHNOLOGY

Microbial Polyhydroxyalkanoates and Nonnatural Polyesters

(2020) So Young Choi et al.   ADVANCED MATERIALS

Biosynthesis of adipic acid by a highly efficient induction-free system in Escherichia coli

(2020) Yu Zhou et al.   JOURNAL OF BIOTECHNOLOGY

Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis

(2020) Lin-Ping Yu et al.   METABOLIC ENGINEERING

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

(2020) Ruchira Mitra et al.   Microbial Cell Factories

Metabolic engineering for the production of dicarboxylic acids and diamines

(2019) Tong Un Chae et al.   METABOLIC ENGINEERING

Protein engineering: the potential of remote mutations

(2019) Matthew Wilding et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Anaerobic Production of Poly(3-hydroxybutyrate) and Its Precursor 3-Hydroxybutyrate from Synthesis Gas by Autotrophic Clostridia

(2019) Sebastian Flüchter et al.   BIOMACROMOLECULES

Increased Tolerance to Furfural by Introduction of Polyhydroxybutyrate Synthetic Genes to Escherichia coli

(2019) Hye-Rim Jung et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Combining Protein and Metabolic Engineering Strategies for High-Level Production of O-Acetylhomoserine in Escherichia coli

(2019) Liang Wei et al.   ACS Synthetic Biology

Directed Evolution of Protein Catalysts

(2018) Cathleen Zeymer et al.   Annual Review of Biochemistry

Engineering Erg10 Thiolase from Saccharomyces cerevisiae as a Synthetic Toolkit for the Production of Branched-Chain Alcohols

(2018) Pamela Torres-Salas et al.   BIOCHEMISTRY

Structural and Biochemical Studies of Substrate Selectivity in Ascaris suum Thiolases

(2018) Michael R. Blaisse et al.   BIOCHEMISTRY

Optimization of hexanoic acid production in recombinant Escherichia coli by precise flux rebalancing

(2018) Seong Gyeong Kim et al.   BIORESOURCE TECHNOLOGY

Selective production of decanoic acid from iterative reversal of β-oxidation pathway

(2018) Seohyoung Kim et al.   BIOTECHNOLOGY AND BIOENGINEERING

Rational design of thiolase substrate specificity for metabolic engineering applications

(2018) Brian M. Bonk et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering microbial fatty acid metabolism for biofuels and biochemicals

(2018) Eko Roy Marella et al.   CURRENT OPINION IN BIOTECHNOLOGY

Advances in bio-based production of dicarboxylic acids longer than C4

(2018) Jia-Le Yu et al.   ENGINEERING IN LIFE SCIENCES

Polyhydroxyalkanoates: Characteristics, production, recent developments and applications

(2018) Zulfiqar Ali Raza et al.   INTERNATIONAL BIODETERIORATION & BIODEGRADATION

Combination of type II fatty acid biosynthesis enzymes and thiolases supports a functional β-oxidation reversal

(2018) James M. Clomburg et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli for producing adipic acid through the reverse adipate-degradation pathway

(2018) Mei Zhao et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source

(2018) Jing Chen et al.   Microbial Cell Factories

Engineering strategies for rational polyketide synthase design

(2018) Maja Klaus et al.   NATURAL PRODUCT REPORTS

Archaeal acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site

(2018) Bastian Vögeli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of Aspergillus fumigatus Cytosolic Thiolase: Trapped Tetrahedral Reaction Intermediates and Activation by Monovalent Cations

(2018) Andrew C. Marshall et al.   ACS Catalysis

Engineering Escherichia coli for Glutarate Production as the C5 Platform Backbone

(2018) Mei Zhao et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Reviving the Weizmann process for commercial n-butanol production

(2018) Ngoc-Phuong-Thao Nguyen et al.   Nature Communications

Engineering Pseudomonas entomophila for synthesis of copolymers with defined fractions of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates

(2018) Mengyi Li et al.   METABOLIC ENGINEERING

Production of Odd-Carbon Dicarboxylic Acids in Escherichia coli Using an Engineered Biotin–Fatty Acid Biosynthetic Pathway

(2017) Robert W. Haushalter et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Discovery and Engineering of Pathways for Production of α-Branched Organic Acids

(2017) Michael R. Blaisse et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A systematic optimization of medium chain fatty acid biosynthesis via the reverse beta-oxidation cycle in Escherichia coli

(2017) Junjun Wu et al.   METABOLIC ENGINEERING

Reversal of β-oxidative pathways for the microbial production of chemicals and polymer building blocks

(2017) Nicolai Kallscheuer et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli for the synthesis of the quadripolymer poly(glycolate- co -lactate- co -3-hydroxybutyrate- co -4-hydroxybutyrate) from glucose

(2017) Zheng-Jun Li et al.   METABOLIC ENGINEERING

Microbial synthesis of medium-chain chemicals from renewables

(2017) Stephen Sarria et al.   NATURE BIOTECHNOLOGY

Crystallographic substrate binding studies ofLeishmania mexicanaSCP2-thiolase (type-2): unique features of oxyanion hole-1

(2017) Rajesh K. Harijan et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Improved production of adipate with Escherichia coli by reversal of β-oxidation

(2016) Nicolai Kallscheuer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Protein Allostery and Conformational Dynamics

(2016) Jingjing Guo et al.   CHEMICAL REVIEWS

Chain Elongation with Reactor Microbiomes: Open-Culture Biotechnology To Produce Biochemicals

(2016) Largus T. Angenent et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

Crystal structure and biochemical characterization of a 3-ketoacyl-CoA thiolase from Ralstoniaeutropha H16

(2016) Jieun Kim et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Engineering Escherichia coli for the synthesis of short- and medium-chain α,β-unsaturated carboxylic acids

(2016) Seohyoung Kim et al.   METABOLIC ENGINEERING

Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions

(2016) Seokjung Cheong et al.   NATURE BIOTECHNOLOGY

Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources

(2016) Kajan Srirangan et al.   Scientific Reports

Crystal structure of a thiolase fromEscherichia coliat 1.8 Å resolution

(2016) M. Ithayaraja et al.   Acta Crystallographica Section F-Structural Biology Communications

Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli

(2016) Chayatip Insomphun et al.   AMB Express

Synthesis and characterisation of bio-based polyester materials from vegetable oil and short to long chain dicarboxylic acids

(2015) Maëlle Gobin et al.   INDUSTRIAL CROPS AND PRODUCTS

Synthesis of medium-chain length (C6–C10) fuels and chemicals via β-oxidation reversal in Escherichia coli

(2015) Seohyoung Kim et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous β-ketoacyl thiolase from Ralstonia eutropha

(2015) Christopher D. Fage et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Alteration of Wax Ester Content and Composition in Euglena gracilis with Gene Silencing of 3-ketoacyl-CoA Thiolase Isozymes

(2015) Masami Nakazawa et al.   LIPIDS

Integrated engineering of β-oxidation reversal and ω-oxidation pathways for the synthesis of medium chain ω-functionalized carboxylic acids

(2015) James M. Clomburg et al.   METABOLIC ENGINEERING

Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum

(2015) Sangwoo Kim et al.   Nature Communications

Engineering Escherichia coli for Conversion of Glucose to Medium-Chain ω-Hydroxy Fatty Acids and α,ω-Dicarboxylic Acids

(2015) Christopher H. Bowen et al.   ACS Synthetic Biology

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase fromMycobacterium smegmatis

(2015) Neelanjana Janardan et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Escherichia coli Enoyl-Acyl Carrier Protein Reductase (FabI) Supports Efficient Operation of a Functional Reversal of the β-Oxidation Cycle

(2014) Jacob E. Vick et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Crystal structure and biochemical characterization of beta-keto thiolase B from polyhydroxyalkanoate-producing bacterium Ralstonia eutropha H16

(2014) Eun-Jung Kim et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Direct biosynthesis of adipic acid from a synthetic pathway in recombinantEscherichia coli

(2014) Jia-Le Yu et al.   BIOTECHNOLOGY AND BIOENGINEERING

A biosynthetic pathway for hexanoic acid production in Kluyveromyces marxianus

(2014) Yuna Cheon et al.   JOURNAL OF BIOTECHNOLOGY

In silico assessment of the metabolic capabilities of an engineered functional reversal of the β-oxidation cycle for the synthesis of longer-chain (C≥4) products

(2014) Angela Cintolesi et al.   METABOLIC ENGINEERING

Accurate design of co-assembling multi-component protein nanomaterials

(2014) Neil P. King et al.   NATURE

Phylogenetic relationships and classification of thiolases and thiolase-like proteins of Mycobacterium tuberculosis and Mycobacterium smegmatis

(2014) Padmanabhan Anbazhagan et al.   TUBERCULOSIS

Reversal of the β-Oxidation Cycle in Saccharomyces cerevisiae for Production of Fuels and Chemicals

(2014) Jiazhang Lian et al.   ACS Synthetic Biology

The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities

(2014) Tiila-Riikka Kiema et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Functional Screening andIn VitroAnalysis Reveal Thioesterases with Enhanced Substrate Specificity Profiles That Improve Short-Chain Fatty Acid Production in Escherichia coli

(2013) Matthew D. McMahon et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

(2013) Collin H. Martin et al.   Nature Communications

Thiolase engineering for enhanced butanol production inClostridium acetobutylicum

(2012) Miriam S. Mann et al.   BIOTECHNOLOGY AND BIOENGINEERING

Chain elongation with reactor microbiomes: upgrading dilute ethanol to medium-chain carboxylates

(2012) Matthew T. Agler et al.   Energy & Environmental Science

A selection platform for carbon chain elongation using the CoA-dependent pathway to produce linear higher alcohols

(2012) Hidevaldo B. Machado et al.   METABOLIC ENGINEERING

Engineering Escherichia coli for production of C12–C14 polyhydroxyalkanoate from glucose

(2012) Daniel E. Agnew et al.   METABOLIC ENGINEERING

Principles for designing ideal protein structures

(2012) Nobuyasu Koga et al.   NATURE

Engineering synthetic recursive pathways to generate non-natural small molecules

(2012) Elizabeth A Felnagle et al.   Nature Chemical Biology

A Synthetic Biology Approach to Engineer a Functional Reversal of the β-Oxidation Cycle

(2012) James M. Clomburg et al.   ACS Synthetic Biology

Production in Escherichia coli of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) with Differing Monomer Compositions from Unrelated Carbon Sources

(2011) Quan Chen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

(2011) Claire R. Shen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Extending Carbon Chain Length of 1-Butanol Pathway for 1-Hexanol Synthesis from Glucose by EngineeredEscherichia coli

(2011) Yasumasa Dekishima et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals

(2011) Clementina Dellomonaco et al.   NATURE

Peroxisomal Plant 3-Ketoacyl-CoA Thiolase Structure and Activity Are Regulated by a Sensitive Redox Switch

(2010) Valerie E. Pye et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biosynthesis of polyketides by trans-AT polyketide synthases

(2010) Jörn Piel   NATURAL PRODUCT REPORTS

Engineering of pha operon on Cupriavidus necator chromosome for efficient biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil

(2010) Jun Mifune et al.   POLYMER DEGRADATION AND STABILITY

Improving computational protein design by using structure-derived sequence profile

(2010) Liang Dai et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

The Thiolase Reaction Mechanism: The Importance of Asn316 and His348 for Stabilizing the Enolate Intermediate of the Claisen Condensation

(2009) Gitte Meriläinen et al.   BIOCHEMISTRY

HotSpot Wizard: a web server for identification of hot spots in protein engineering

(2009) A. Pavelka et al.   NUCLEIC ACIDS RESEARCH

Improved Thermostability and Acetic Acid Tolerance of Escherichia coli via Directed Evolution of Homoserine o-Succinyltransferase

(2008) E. A. Mordukhova et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme

(2008) Gitte Meriläinen et al.   FEBS Journal

Enzyme stability and stabilization—Aqueous and non-aqueous environment

(2008) Padma V. Iyer et al.   PROCESS BIOCHEMISTRY