Multidimensional control of therapeutic human cell function with synthetic gene circuits

Modular design of synthetic receptors for programmed gene regulation in cell therapies

(2022) Iowis Zhu et al.   CELL

Single-cell antigen-specific landscape of CAR T infusion product identifies determinants of CD19-positive relapse in patients with ALL

(2022) Zhiliang Bai et al.   Science Advances

Transient rest restores functionality in exhausted CAR-T cells through epigenetic remodeling

(2021) Evan W. Weber et al.   SCIENCE

SynNotch CAR circuits enhance solid tumor recognition and promote persistent antitumor activity in mouse models

(2021) Axel Hyrenius-Wittsten et al.   Science Translational Medicine

SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma

(2021) Joseph H. Choe et al.   Science Translational Medicine

Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing

(2021) Xiaoshu Xu et al.   MOLECULAR CELL

Enhancing Chimeric Antigen Receptor T cell Efficacy in Solid Tumors

(2020) Giovanni Fucá et al.   CLINICAL CANCER RESEARCH

The COMET toolkit for composing customizable genetic programs in mammalian cells

(2020) Patrick S. Donahue et al.   Nature Communications

Engineering CAR-T Cells for Next-Generation Cancer Therapy

(2020) Mihe Hong et al.   CANCER CELL

All systems go: converging synthetic biology and combinatorial treatment for CAR-T cell therapy

(2020) Evripidis Lanitis et al.   CURRENT OPINION IN BIOTECHNOLOGY

TGF-β suppresses type 2 immunity to cancer

(2020) Ming Liu et al.   NATURE

Identification of preexisting adaptive immunity to Cas9 proteins in humans

(2019) Carsten T. Charlesworth et al.   NATURE MEDICINE

Pharmacokinetic Interactions between the Hepatitis C Virus Inhibitors Elbasvir and Grazoprevir and HIV Protease Inhibitors Ritonavir, Atazanavir, Lopinavir, and Darunavir in Healthy Volunteers

(2019) Hwa-Ping Feng et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies

(2019) Caleb J. Bashor et al.   SCIENCE

De novo design of bioactive protein switches

(2019) Robert A. Langan et al.   NATURE

CAR-T cells secreting BiTEs circumvent antigen escape without detectable toxicity

(2019) Bryan D. Choi et al.   NATURE BIOTECHNOLOGY

Supercharging adoptive T cell therapy to overcome solid tumor–induced immunosuppression

(2019) Sherly Mardiana et al.   Science Translational Medicine

Engineering strategies to overcome the current roadblocks in CAR T cell therapy

(2019) Sarwish Rafiq et al.   Nature Reviews Clinical Oncology

Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Cell Responses

(2018) Jang Hwan Cho et al.   CELL

CD28-ζ CAR T cells resist TGF-β repression through IL-2 signaling which can be mimicked by IL-7 and an engineered IL-7/IL-2Rβ autocrine loop

(2018) Viktória Golumba-Nagy et al.   MOLECULAR THERAPY

StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins

(2018) Conor L. Jacobs et al.   NATURE METHODS

Chemogenetic control of gene expression and cell signaling with antiviral drugs

(2018) Elliot P. Tague et al.   NATURE METHODS

Designing cell function: assembly of synthetic gene circuits for cell biology applications

(2018) Mingqi Xie et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Programming gene and engineered-cell therapies with synthetic biology

(2018) Tasuku Kitada et al.   SCIENCE

Programmable protein circuits in living cells

(2018) Xiaojing J. Gao et al.   SCIENCE

Cytokine release syndrome and neurotoxicity after CD19 chimeric antigen receptor-modified (CAR-) T cell therapy

(2018) Kevin A. Hay   BRITISH JOURNAL OF HAEMATOLOGY

The Human Transcription Factors

(2018) Samuel A. Lambert et al.   CELL

Engineering Epigenetic Regulation Using Synthetic Read-Write Modules

(2018) Minhee Park et al.   CELL

Programmable design of orthogonal protein heterodimers

(2018) Zibo Chen et al.   NATURE

High prevalence of Streptococcus pyogenes Cas9-reactive T cells within the adult human population

(2018) Dimitrios L. Wagner et al.   NATURE MEDICINE

Augmentation of Antitumor Immunity by Human and Mouse CAR T Cells Secreting IL-18

(2017) Biliang Hu et al.   Cell Reports

High-Content Monitoring of Drug Effects in a 3D Spheroid Model

(2017) Frédérique Mittler et al.   Frontiers in Oncology

Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits

(2016) Kole T. Roybal et al.   CELL

Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors

(2016) Kole T. Roybal et al.   CELL

 -cell-mimetic designer cells provide closed-loop glycemic control

(2016) M. Xie et al.   SCIENCE

Quantitative Analyses of Core Promoters Enable Precise Engineering of Regulated Gene Expression in Mammalian Cells

(2016) Christopher Ede et al.   ACS Synthetic Biology

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Tumor-Infiltrating Lymphocytes Genetically Engineered with an Inducible Gene Encoding Interleukin-12 for the Immunotherapy of Metastatic Melanoma

(2015) L. Zhang et al.   CLINICAL CANCER RESEARCH

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis

(2015) Lina Schukur et al.   Science Translational Medicine

Efficacy and safety of grazoprevir (MK-5172) and elbasvir (MK-8742) in patients with hepatitis C virus and HIV co-infection (C-EDGE CO-INFECTION): a non-randomised, open-label trial

(2015) Jürgen K Rockstroh et al.   Lancet HIV

R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences

(2014) Arturo Casini et al.   ACS Synthetic Biology

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

RNA-guided gene activation by CRISPR-Cas9–based transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

Cell-Based Therapeutics: The Next Pillar of Medicine

(2013) M. A. Fischbach et al.   Science Translational Medicine

Properties of MHC Class I Presented Peptides That Enhance Immunogenicity

(2013) Jorg J. A. Calis et al.   PLoS Computational Biology

A Synthetic Biology Framework for Programming Eukaryotic Transcription Functions

(2012) Ahmad S. Khalil et al.   CELL

Exploiting a natural conformational switch to engineer an interleukin-2 ‘superkine’

(2012) Aron M. Levin et al.   NATURE

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

p53 Dynamics Control Cell Fate

(2012) J. E. Purvis et al.   SCIENCE

Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity

(2011) F.-S. Liang et al.   Science Signaling

Case Report of a Serious Adverse Event Following the Administration of T Cells Transduced With a Chimeric Antigen Receptor Recognizing ERBB2

(2010) Richard A Morgan et al.   MOLECULAR THERAPY

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)

(2010) Jeffry D Sander et al.   NATURE METHODS

Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays

(2009) Morgan L Maeder et al.   Nature Protocols

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

(2009) Ben Langmead et al.   GENOME BIOLOGY

Frequency-modulated nuclear localization bursts coordinate gene regulation

(2008) Long Cai et al.   NATURE