Recent advances in gene therapy for bone tissue engineering

rAAV-Mediated Human FGF-2 Gene Therapy Enhances Osteochondral Repair in a Clinically Relevant Large Animal Model Over Time In Vivo

(2021) Yannik P. Morscheid et al.   AMERICAN JOURNAL OF SPORTS MEDICINE

Bringing a Gene-Activated Bone Substitute Into Clinical Practice: From Bench to Bedside

(2021) Ilia Y. Bozo et al.   Frontiers in Bioengineering and Biotechnology

Gene- and RNAi-Activated Scaffolds for Bone Tissue Engineering: Current Progress and Future Directions

(2021) Noah Z. Laird et al.   ADVANCED DRUG DELIVERY REVIEWS

In Vivo bone tissue induction by freeze-dried collagen-nanohydroxyapatite matrix loaded with BMP2/NS1 mRNAs lipopolyplexes

(2021) Pinpin Wang et al.   JOURNAL OF CONTROLLED RELEASE

Gene Therapy for Bone Healing: Lessons Learned and New Approaches

(2021) R.E. De la Vega et al.   Translational Research

Systematic Review of the Preclinical Technology Readiness of Orthopedic Gene Therapy and Outlook for Clinical Translation

(2021) Piers Wilkinson et al.   Frontiers in Bioengineering and Biotechnology

CRISPR activation of long non-coding RNA DANCR promotes bone regeneration

(2021) Nuong Thi Kieu Nguyen et al.   BIOMATERIALS

Regional gene therapy for bone healing using a 3D printed scaffold in a rat femoral defect model

(2021) H Paco Kang et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

An Overview of RNA-Based Scaffolds for Osteogenesis

(2021) Laila A. Damiati et al.   Frontiers in Molecular Biosciences

Increasing Gene Editing Efficiency for CRISPR-Cas9 by Small RNAs in Pluripotent Stem Cells

(2021) Alireza Shahryari et al.   CRISPR Journal

Development of a three-layer consecutive gene delivery system for enhanced bone regeneration

(2021) Hye Jin Kim et al.   BIOMATERIALS

Bone tissue engineering using 3‐D polycaprolactone/gelatin nanofibrous scaffold containing berberine: In vivo and in vitro study

(2021) Arian Ehterami et al.   POLYMERS FOR ADVANCED TECHNOLOGIES

Hierarchical assembly of nanostructured coating for siRNA-based dual therapy of bone regeneration and revascularization

(2020) Helin Xing et al.   BIOMATERIALS

Retroviral Insertional Mutagenesis in Humans: Evidence for Four Genetic Mechanisms Promoting Expansion of Cell Clones

(2020) Frederic D. Bushman   MOLECULAR THERAPY

Health ministers condemn Novartis lottery for Zolgensma, the world’s most expensive drug

(2020) Owen Dyer   BMJ-British Medical Journal

In situ bone tissue engineering using gene delivery nanocomplexes

(2020) Atefeh Malek-Khatabi et al.   Acta Biomaterialia

Electrospun gelatin matrices with bioactive pDNA polyplexes

(2020) Porntipa Pankongadisak et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Cell–cell interaction in a co‐culture system consisting of CRISPR /Cas9 mediated GFP knock‐ in HUVECs and MG ‐63 cells in alginate‐GelMA based nanocomposites hydrogel as a 3D scaffold

(2020) Fahimeh Shahabipour et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Concise review on optimized methods in production and transduction of lentiviral vectors in order to facilitate immunotherapy and gene therapy

(2020) Hadi Esmaeili Gouvarchin Ghaleh et al.   BIOMEDICINE & PHARMACOTHERAPY

Microarray analysis reveals that lncRNA PWRN1 ‐209 promotes human bone marrow mesenchymal stem cell osteogenic differentiation on microtopography titanium surface in vitro

(2020) Mingyue Wang et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

Generation of a human iPSC line harboring a biallelic large deletion at the INK4 locus (HMGUi001-A-5)

(2020) Alireza Shahryari et al.   Stem Cell Research

CRISPR interference-mediated noggin knockdown promotes BMP2-induced osteogenesis and calvarial bone healing

(2020) Mu-Nung Hsu et al.   BIOMATERIALS

Ex vivo regional gene therapy with human adipose-derived stem cells for bone repair

(2020) Venus Vakhshori et al.   BONE

Non-viral gene delivery embedded in biomimetically mineralized matrices for bone tissue engineering

(2020) Timothy Acri et al.   TISSUE ENGINEERING PART A

Bone Regeneration: A Multi‐Functional Implant Induces Bone Formation in a Diabetic Model (Adv. Healthcare Mater. 18/2020)

(2020) Behnoush Khorsand et al.   Advanced Healthcare Materials

Non-viral Gene Delivery of Interleukin-1 Receptor Antagonist Using Collagen-Hydroxyapatite Scaffold Protects Rat BM-MSCs From IL-1β-Mediated Inhibition of Osteogenesis

(2020) William A. Lackington et al.   Frontiers in Bioengineering and Biotechnology

Trb3 controls mesenchymal stem cell lineage fate and enhances bone regeneration by scaffold-mediated local gene delivery

(2020) Jiabing Fan et al.   BIOMATERIALS

Demineralized bone matrix scaffold modified with mRNA derived from osteogenically pre-differentiated MSCs improves bone repair

(2020) Qiuping Leng et al.   Materials Science & Engineering C-Materials for Biological Applications

A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection

(2020) Minqi Wang et al.   Bioactive Materials

LOC103691336/miR‐138‐5p/BMPR2 axis modulates Mg‐mediated osteogenic differentiation in rat femoral fracture model and rat primary bone marrow stromal cells

(2019) Ding Li et al.   JOURNAL OF CELLULAR PHYSIOLOGY

CRISPRai for simultaneous gene activation and inhibition to promote stem cell chondrogenesis and calvarial bone regeneration

(2019) Vu Anh Truong et al.   NUCLEIC ACIDS RESEARCH

Mesenchymal stem cell-associated lncRNA in osteogenic differentiation

(2019) Cheng Ju et al.   BIOMEDICINE & PHARMACOTHERAPY

The Application of microRNAs in Biomaterial Scaffold‐Based Therapies for Bone Tissue Engineering

(2019) Marco A. Arriaga et al.   Biotechnology Journal

Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering

(2019) Rosanne M. Raftery et al.   BIOMATERIALS

Development and Clinical Translation of Approved Gene Therapy Products for Genetic Disorders

(2019) Alireza Shahryari et al.   Frontiers in Genetics

RNA‐binding protein PUM2 regulates mesenchymal stem cell fate via repression of JAK2 and RUNX2 mRNAs

(2019) Myon‐Hee Lee et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Electroactive composite scaffold with locally expressed osteoinductive factor for synergistic bone repair upon electrical stimulation

(2019) Liguo Cui et al.   BIOMATERIALS

Collagen-infilled 3D printed scaffolds loaded with miR-148b-transfected bone marrow stem cells improve calvarial bone regeneration in rats

(2019) Kazim K. Moncal et al.   Materials Science & Engineering C-Materials for Biological Applications

Coactivation of Endogenous Wnt10b and Foxc2 by CRISPR Activation Enhances BMSC Osteogenesis and Promotes Calvarial Bone Regeneration

(2019) Mu-Nung Hsu et al.   MOLECULAR THERAPY

RNA interfering molecule delivery from in situ forming biodegradable hydrogels for enhancement of bone formation in rat calvarial bone defects

(2018) Minh K. Nguyen et al.   Acta Biomaterialia

Improving Bone Regeneration Using Chordin siRNA Delivered by pH-Responsive and Non-Toxic Polyspermine Imidazole-4,5-Imine

(2018) Chuandong Wang et al.   CELLULAR PHYSIOLOGY AND BIOCHEMISTRY

CRISPR RNAs trigger innate immune responses in human cells

(2018) Sojung Kim et al.   GENOME RESEARCH

Chitosan/nano-hydroxyapatite/nano-zirconium dioxide scaffolds with miR-590-5p for bone regeneration

(2018) K. Balagangadharan et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Delivery of the improved BMP-2-Advanced plasmid DNA within a gene-activated scaffold accelerates mesenchymal stem cell osteogenesis and critical size defect repair

(2018) Rosanne M. Raftery et al.   JOURNAL OF CONTROLLED RELEASE

BMP2 expressing genetically engineered mesenchymal stem cells on composite fibrous scaffolds for enhanced bone regeneration in segmental defects

(2018) Shruthy Kuttappan et al.   Materials Science & Engineering C-Materials for Biological Applications

BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model

(2018) Ming-Kai Hsieh et al.   Materials Science & Engineering C-Materials for Biological Applications

Improvement of Bone Healing by Neutralization of microRNA-335-5p, but not by Neutralization of microRNA-92A in Bone Marrow Mononuclear Cells Transplanted into a Large Femur Defect of the Rat

(2018) Maren Janko et al.   TISSUE ENGINEERING PART A

Harnessing a Novel Inhibitory Role of miR-16 in Osteogenesis by Human Mesenchymal Stem Cells for Advanced Scaffold-Based Bone Tissue Engineering

(2018) Irene Mencía Castaño et al.   TISSUE ENGINEERING PART A

An improved, chemically modified RNA encoding BMP-2 enhances osteogenesis in vitro and in vivo

(2018) Wen Zhang et al.   TISSUE ENGINEERING PART A

Bone Response to Titanium Implants Coated with Double- or Single-Stranded DNA

(2018) Nagahiro Miyamoto et al.   Biomed Research International

Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice

(2018) Zhi Liu et al.   Molecular Medicine Reports

Improved Bone Regeneration With Multiporous PLGA Scaffold and BMP-2–Transduced Human Adipose-Derived Stem Cells by Cell-Permeable Peptide

(2017) Suhyun Park et al.   Implant Dentistry

Novel Synthesized Nanofibrous Scaffold Efficiently Delivered hBMP-2 Encoded in Adenoviral Vector to Promote Bone Regeneration

(2017) Yang Zhu et al.   Journal of Biomedical Nanotechnology

Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model

(2017) Behnoush Khorsand et al.   JOURNAL OF CONTROLLED RELEASE

Ultrasound-responsive gene-activated matrices for osteogenic gene therapy using matrix-assisted sonoporation

(2017) N. Nomikou et al.   Journal of Tissue Engineering and Regenerative Medicine

Modified mRNA for BMP-2 in Combination with Biomaterials Serves as a Transcript-Activated Matrix for Effectively Inducing Osteogenic Pathways in Stem Cells

(2017) Elizabeth R. Balmayor et al.   STEM CELLS AND DEVELOPMENT

Effects of miR-146a on the osteogenesis of adipose-derived mesenchymal stem cells and bone regeneration

(2017) Qing Xie et al.   Scientific Reports

Osteoprotegerin gene-modified BMSCs with hydroxyapatite scaffold for treating critical-sized mandibular defects in ovariectomized osteoporotic rats

(2016) Xian Liu et al.   Acta Biomaterialia

The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration

(2016) Qing Xie et al.   BIOMATERIALS

Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model

(2016) Li-Zhen Zheng et al.   BONE

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

(2016) Yimin Zhao et al.   International Journal of Nanomedicine

Transcript-activated collagen matrix as sustained mRNA delivery system for bone regeneration

(2016) Zohreh Sadat Badieyan et al.   JOURNAL OF CONTROLLED RELEASE

A multifunctional AAV–CRISPR–Cas9 and its host response

(2016) Wei Leong Chew et al.   NATURE METHODS

Mussel Adhesion-Inspired Reverse Transfection Platform Enhances Osteogenic Differentiation and Bone Formation of Human Adipose-Derived Stem Cells

(2016) Jisoo Shin et al.   Small

Fabrication of Core-Shell PEI/pBMP2-PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells

(2016) Qiao Xie et al.   Stem Cells International

Bone scaffolds loaded with siRNA-Semaphorin4d for the treatment of osteoporosis related bone defects

(2016) Yufeng Zhang et al.   Scientific Reports

Photoactivated miR-148b–nanoparticle conjugates improve closure of critical size mouse calvarial defects

(2015) Ammar T. Qureshi et al.   Acta Biomaterialia

A Pilot Study Evaluating Combinatorial and Simultaneous Delivery of Polyethylenimine-Plasmid DNA Complexes Encoding for VEGF and PDGF for Bone Regeneration in Calvarial Bone Defects

(2015) Sheetal D'Mello et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Development of collagen–hydroxyapatite scaffolds incorporating PLGA and alginate microparticles for the controlled delivery of rhBMP-2 for bone tissue engineering

(2015) Elaine Quinlan et al.   JOURNAL OF CONTROLLED RELEASE

Chemically modified RNA activated matrices enhance bone regeneration

(2015) Satheesh Elangovan et al.   JOURNAL OF CONTROLLED RELEASE

Enhanced bone healing using collagen-hydroxyapatite scaffold implantation in the treatment of a large multiloculated mandibular aneurysmal bone cyst in a thoroughbred filly

(2015) Florent David et al.   Journal of Tissue Engineering and Regenerative Medicine

Controlled release of vascular endothelial growth factor from spray-dried alginate microparticles in collagen-hydroxyapatite scaffolds for promoting vascularization and bone repair

(2015) Elaine Quinlan et al.   Journal of Tissue Engineering and Regenerative Medicine

Functionalization of a Collagen-Hydroxyapatite Scaffold with Osteostatin to Facilitate Enhanced Bone Regeneration

(2015) Elaine Quinlan et al.   Advanced Healthcare Materials

Osteochondral tissue regeneration through polymeric delivery of DNA encoding for the SOX trio and RUNX2

(2014) Clark J. Needham et al.   Acta Biomaterialia

Osteogenic differentiation of adipose-derived stem cells and calvarial defect repair using baculovirus-mediated co-expression of BMP-2 and miR-148b

(2014) Ya-Hsin Liao et al.   BIOMATERIALS

Adenovirus: The First Effective In Vivo Gene Delivery Vector

(2014) Ronald G. Crystal   HUMAN GENE THERAPY

Incorporation of osteogenic and angiogenic small interfering RNAs into chitosan sponge for bone tissue engineering

(2014) Bin Lu et al.   International Journal of Nanomedicine

Osteoclast progenitors promote bone vascularization and osteogenesis

(2014) Anjali P. Kusumbe et al.   NATURE MEDICINE

PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis

(2014) Hui Xie et al.   NATURE MEDICINE

Inhibition of osteoclastogenesis and inflammatory bone resorption by targeting BET proteins and epigenetic regulation

(2014) Kyung-Hyun Park-Min et al.   Nature Communications

Biomineral Coating Increases Bone Formation by Ex Vivo BMP-7 Gene Therapy in Rapid Prototyped Poly(l-lactic acid) (PLLA) and Poly(ε-caprolactone) (PCL) Porous Scaffolds

(2014) Eiji Saito et al.   Advanced Healthcare Materials

The promotion of bone regeneration through positive regulation of angiogenic–osteogenic coupling using microRNA-26a

(2013) Yan Li et al.   BIOMATERIALS

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

MicroRNA-34a Inhibits Osteoblast Differentiation and In Vivo Bone Formation of Human Stromal Stem Cells

(2013) Li Chen et al.   STEM CELLS

Sonoporation Increases Therapeutic Efficacy of Inducible and Constitutive BMP2/7 In Vivo Gene Delivery

(2013) Georg A. Feichtinger et al.   Human Gene Therapy Methods

Immune responses during healing of massive segmental femoral bone defects mediated by hybrid baculovirus-engineered ASCs

(2012) Chin-Yu Lin et al.   BIOMATERIALS

Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration

(2012) Yufeng Zhang et al.   BONE

miR-214 targets ATF4 to inhibit bone formation

(2012) Xiaogang Wang et al.   NATURE MEDICINE

Repairing critical-sized calvarial defects with BMSCs modified by a constitutively active form of hypoxia-inducible factor-1α and a phosphate cement scaffold

(2011) Duohong Zou et al.   BIOMATERIALS

Angiogenesis and osteogenesis enhanced by bFGF ex vivo gene therapy for bone tissue engineering in reconstruction of calvarial defects

(2011) Dan Qu et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

MicroRNA-138 regulates osteogenic differentiation of human stromal (mesenchymal) stem cells in vivo

(2011) T. Eskildsen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The ability of a collagen/calcium phosphate scaffold to act as its own vector for gene delivery and to promote bone formation via transfection with VEGF165

(2009) Michael Keeney et al.   BIOMATERIALS

Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality of bone repair in a rat femoral defect model

(2008) Mandeep Singh Virk et al.   BONE

Comparison of Lentiviral and Adenoviral Gene Therapy for Spinal Fusion in Rats

(2008) Masashi Miyazaki et al.   SPINE