CRISPRai for simultaneous gene activation and inhibition to promote stem cell chondrogenesis and calvarial bone regeneration

Identification of preexisting adaptive immunity to Cas9 proteins in humans

(2019) Carsten T. Charlesworth et al.   NATURE MEDICINE

An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resistance

(2018) Assaf C. Bester et al.   CELL

CRISPR-Based Chromatin Remodeling of the Endogenous Oct4 or Sox2 Locus Enables Reprogramming to Pluripotency

(2018) Peng Liu et al.   Cell Stem Cell

CRISPR RNAs trigger innate immune responses in human cells

(2018) Sojung Kim et al.   GENOME RESEARCH

In Situ Gene Therapy via AAV-CRISPR-Cas9-Mediated Targeted Gene Regulation

(2018) Ana M. Moreno et al.   MOLECULAR THERAPY

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

An enhanced CRISPR repressor for targeted mammalian gene regulation

(2018) Nan Cher Yeo et al.   NATURE METHODS

Synthetic switch-based baculovirus for transgene expression control and selective killing of hepatocellular carcinoma cells

(2018) Mei-Wei Lin et al.   NUCLEIC ACIDS RESEARCH

Human pluripotent reprogramming with CRISPR activators

(2018) Jere Weltner et al.   Nature Communications

Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9

(2018) Lupeng Ye et al.   Cell Discovery

CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming

(2018) Yanxia Liu et al.   Cell Stem Cell

SOX2 Activation Using CRISPR/dCas9 Promotes Wound Healing in Corneal Endothelial Cells

(2018) Yoon Kyung Chang et al.   STEM CELLS

Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference

(2018) Ashley RG Libby et al.   eLife

Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent

(2017) Marco Jost et al.   MOLECULAR CELL

Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood

(2017) Julia Joung et al.   NATURE

Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens

(2017) Fadi J Najm et al.   NATURE BIOTECHNOLOGY

Genetic interaction mapping in mammalian cells using CRISPR interference

(2017) Dan Du et al.   NATURE METHODS

In situ bone tissue engineering via ultrasound-mediated gene delivery to endogenous progenitor cells in mini-pigs

(2017) Maxim Bez et al.   Science Translational Medicine

Engineering cell signaling using tunable CRISPR–Cpf1-based transcription factors

(2017) Yuchen Liu et al.   Nature Communications

Control of Adipogenic Differentiation in Mesenchymal Stem Cells via Endogenous Gene Activation Using CRISPR-Cas9

(2017) Yuichi Furuhata et al.   ACS Synthetic Biology

Enhancing Protein Production Yield from Chinese Hamster Ovary Cells by CRISPR Interference

(2017) Chih-Che Shen et al.   ACS Synthetic Biology

Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators

(2017) Zehua Bao et al.   ACS Synthetic Biology

One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes

(2017) Shenglan Li et al.   Molecular Therapy-Nucleic Acids

Healing of osteoporotic bone defects by baculovirus-engineered bone marrow-derived MSCs expressing MicroRNA sponges

(2016) Kuei-Chang Li et al.   BIOMATERIALS

Targeted Epigenetic Remodeling of Endogenous Loci by CRISPR/Cas9-Based Transcriptional Activators Directly Converts Fibroblasts to Neuronal Cells

(2016) Joshua B. Black et al.   Cell Stem Cell

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs

(2016) Mohammad A. Mandegar et al.   Cell Stem Cell

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

(2016) Yuchen Gao et al.   NATURE METHODS

A multifunctional AAV–CRISPR–Cas9 and its host response

(2016) Wei Leong Chew et al.   NATURE METHODS

Comparison of Cas9 activators in multiple species

(2016) Alejandro Chavez et al.   NATURE METHODS

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

PPARγ suppressed Wnt/β-catenin signaling pathway and its downstream effector SOX9 expression in gastric cancer cells

(2015) Xiyun Ren et al.   MEDICAL ONCOLOGY

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

(2015) Liliana Wroblewska et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Efficient gene delivery into cell lines and stem cells using baculovirus

(2014) Li-Yu Sung et al.   Nature Protocols

A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification

(2014) Syandan Chakraborty et al.   Stem Cell Reports

The use of ASCs engineered to express BMP2 or TGF-β3 within scaffold constructs to promote calvarial bone repair

(2013) Chin-Yu Lin et al.   BIOMATERIALS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Adaptive Immune Responses Elicited by Baculovirus and Impacts on Subsequent Transgene Expression In Vivo

(2013) W.-Y. Luo et al.   JOURNAL OF VIROLOGY

Baculovirus: an Insect-derived Vector for Diverse Gene Transfer Applications

(2013) Kari J Airenne et al.   MOLECULAR THERAPY

Regenerating Cartilages by Engineered ASCs: Prolonged TGF-β3/BMP-6 Expression Improved Articular Cartilage Formation and Restored Zonal Structure

(2013) Chia-Hsin Lu et al.   MOLECULAR THERAPY

Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study

(2013) Li-Yu Sung et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Immune responses during healing of massive segmental femoral bone defects mediated by hybrid baculovirus-engineered ASCs

(2012) Chin-Yu Lin et al.   BIOMATERIALS

Augmented healing of critical-size calvarial defects by baculovirus-engineered MSCs that persistently express growth factors

(2012) Chin-Yu Lin et al.   BIOMATERIALS

Programming human pluripotent stem cells into white and brown adipocytes

(2012) Tim Ahfeldt et al.   NATURE CELL BIOLOGY

Unlike Bone, Cartilage Regeneration Remains Elusive

(2012) D. J. Huey et al.   SCIENCE

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part I: Recapitulation of Native Tissue Healing and Variables for the Design of Delivery Systems

(2012) Vítor E. Santo et al.   TISSUE ENGINEERING PART B-REVIEWS

Biosafety Assessment of Human Mesenchymal Stem Cells Engineered by Hybrid Baculovirus Vectors

(2011) Chi-Yuan Chen et al.   MOLECULAR PHARMACEUTICS

Cranial bone defects: current and future strategies

(2010) Caroline Szpalski et al.   Neurosurgical Focus