Anti-CRISPR Discovery: Using Magnets to Find Needles in Haystacks

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing

(2022) Guoxu Song et al.   NUCLEIC ACIDS RESEARCH

Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages

(2022) Matthew C. Johnson et al.   MOLECULAR CELL

Anti-CRISPR prediction using deep learning reveals an inhibitor of Cas13b nucleases

(2022) Katharina G. Wandera et al.   MOLECULAR CELL

Structural biology of CRISPR–Cas immunity and genome editing enzymes

(2022) Joy Y. Wang et al.   NATURE REVIEWS MICROBIOLOGY

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24

(2022) Lingguang Yang et al.   Nature Communications

Systematic and quantitative view of the antiviral arsenal of prokaryotes

(2022) Florian Tesson et al.   Nature Communications

A truncated anti-CRISPR protein prevents spacer acquisition but not interference

(2022) Cécile Philippe et al.   Nature Communications

Cyclic Nucleotide Signaling in Phage Defense and Counter-Defense

(2022) Januka S. Athukoralage et al.   Annual Review of Virology

Dual inhibition of innate immunity and apoptosis by human cytomegalovirus protein UL37x1 enables efficient virus replication

(2022) Yujie Ren et al.   Nature Microbiology

Widespread repression of anti-CRISPR production by anti-CRISPR-associated proteins

(2022) Saadlee Shehreen et al.   NUCLEIC ACIDS RESEARCH

The evolution of a counter-defense mechanism in a virus constrains its host range

(2022) Sriram Srikant et al.   eLife

Structural basis of AcrIF24 as an anti-CRISPR protein and transcriptional suppressor

(2022) Indranil Arun Mukherjee et al.   Nature Chemical Biology

Anti-CRISPRs go viral: the infection biology of CRISPR-Cas inhibitors

(2021) Yuping Li et al.   Cell Host & Microbe

Clostridioides difficile Phage Biology and Application

(2021) Joshua Heuler et al.   FEMS MICROBIOLOGY REVIEWS

Mobile element warfare via CRISPR and anti-CRISPR in Pseudomonas aeruginosa

(2021) Lina M León et al.   NUCLEIC ACIDS RESEARCH

Cyclic oligoadenylate signalling and regulation by ring nucleases during type III CRISPR defence

(2021) Januka S Athukoralage et al.   RNA

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins

(2021) Ning Jia et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

CRISPR diagnostics

(2021) Omar O. Abudayyeh et al.   SCIENCE

Type III CRISPR-Cas Systems: Deciphering the Most Complex Prokaryotic Immune System

(2021) Matvey V. Kolesnik et al.   BIOCHEMISTRY-MOSCOW

Viral recombination systems limit CRISPR-Cas targeting through the generation of escape mutations

(2021) Amer A. Hossain et al.   Cell Host & Microbe

Identification and classification of antiviral defence systems in bacteria and archaea with PADLOC reveals new system types

(2021) Leighton J Payne et al.   NUCLEIC ACIDS RESEARCH

Inhibition mechanisms of CRISPR-Cas9 by AcrIIA17 and AcrIIA18

(2021) Xiaoshen Wang et al.   NUCLEIC ACIDS RESEARCH

The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity

(2021) Kevin J. Forsberg et al.   PLOS BIOLOGY

Prophage integration into CRISPR loci enables evasion of antiviral immunity in Streptococcus pyogenes

(2021) Andrew Varble et al.   Nature Microbiology

An anti-CRISPR viral ring nuclease subverts type III CRISPR immunity

(2020) Januka S. Athukoralage et al.   NATURE

Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems

(2020) Alan R. Davidson et al.   Annual Review of Biochemistry

Critical Anti-CRISPR Locus Repression by a Bi-functional Cas9 Inhibitor

(2020) Beatriz A. Osuna et al.   Cell Host & Microbe

Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes

(2020) Beatriz A. Osuna et al.   Cell Host & Microbe

CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells

(2020) Ping Lin et al.   MOLECULAR CELL

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

(2020) Nicole D. Marino et al.   NATURE METHODS

Machine learning predicts new anti-CRISPR proteins

(2020) Simon Eitzinger et al.   NUCLEIC ACIDS RESEARCH

Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence

(2020) Artem Isaev et al.   NUCLEIC ACIDS RESEARCH

Potent CRISPR-Cas9 inhibitors fromStaphylococcusgenomes

(2020) Kyle E. Watters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity

(2020) Alexander J. Meeske et al.   SCIENCE

In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials

(2020) Kurt Selle et al.   mBio

AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

(2020) Marscha Hirschi et al.   Nature Communications

Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer

(2020) Caroline Mahendra et al.   Nature Microbiology

Functional and Genomic Variation between Human-Derived Isolates of Lachnospiraceae Reveals Inter- and Intra-Species Diversity

(2020) Matthew T. Sorbara et al.   Cell Host & Microbe

IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR–Cas during conjugation

(2020) David Roy et al.   NUCLEIC ACIDS RESEARCH

Anti-CRISPR Proteins in Archaea

(2020) Xu Peng et al.   TRENDS IN MICROBIOLOGY

Machine-learning approach expands the repertoire of anti-CRISPR protein families

(2020) Ayal B. Gussow et al.   Nature Communications

Controlling and enhancing CRISPR systems

(2020) Haridha Shivram et al.   Nature Chemical Biology

(Ph)ighting Phages: How Bacteria Resist Their Parasites

(2019) Jakob T. Rostøl et al.   Cell Host & Microbe

Discovery and Characterization of Cas9 Inhibitors Disseminated across Seven Bacterial Phyla

(2019) Ruben V. Uribe et al.   Cell Host & Microbe

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

(2019) Katharina G. Wandera et al.   METHODS

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Broad-spectrum enzymatic inhibition of CRISPR-Cas12a

(2019) Gavin J. Knott et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An anti-CRISPR protein disables type V Cas12a by acetylation

(2019) Liyong Dong et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Origins and evolution of CRISPR-Cas systems

(2019) Eugene V. Koonin et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins

(2019) Heng Zhang et al.   Cell Host & Microbe

Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription

(2019) Sabrina Y. Stanley et al.   CELL

The autoregulator Aca2 mediates anti-CRISPR repression

(2019) Nils Birkholz et al.   NUCLEIC ACIDS RESEARCH

Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a

(2019) Gavin J Knott et al.   eLife

Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome

(2019) Kevin J Forsberg et al.   eLife

Defining the human gut host–phage network through single-cell viral tagging

(2019) Mária Džunková et al.   Nature Microbiology

Bioinformatics Identification of Anti-CRISPR Loci by Using Homology, Guilt-by-Association, and CRISPR Self-Targeting Spacer Approaches

(2019) Yanbin Yin et al.   mSystems

Inhibition of Type III CRISPR-Cas Immunity by an Archaeal Virus-Encoded Anti-CRISPR Protein

(2019) Yuvaraj Bhoobalan-Chitty et al.   CELL

The pan-immune system of bacteria: antiviral defence as a community resource

(2019) Aude Bernheim et al.   NATURE REVIEWS MICROBIOLOGY

AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage

(2019) Guoxu Song et al.   Cell Reports

Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing

(2019) Bianca Garcia et al.   Cell Reports

A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases

(2019) Senén D. Mendoza et al.   NATURE

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids

(2019) Rafael Pinilla-Redondo et al.   NUCLEIC ACIDS RESEARCH

A jumbo phage that forms a nucleus-like structure evades CRISPR–Cas DNA targeting but is vulnerable to type III RNA-based immunity

(2019) Lucia M. Malone et al.   Nature Microbiology

Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR–Cas Systems

(2018) Mitchell R. O'Connell   JOURNAL OF MOLECULAR BIOLOGY

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

(2018) Ryan Marshall et al.   MOLECULAR CELL

Tolerance of Sulfolobus SMV1 virus to the immunity of I-A and III-B CRISPR-Cas systems in Sulfolobus islandicus

(2018) Tong Guo et al.   RNA Biology

Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins

(2018) Alexander P. Hynes et al.   Nature Communications

Current CRISPR gene drive systems are likely to be highly invasive in wild populations

(2018) Charleston Noble et al.   eLife

Anti-CRISPR proteins encoded by archaeal lytic viruses inhibit subtype I-D immunity

(2018) Fei He et al.   Nature Microbiology

Discovery of widespread Type I and Type V CRISPR-Cas inhibitors

(2018) Nicole D. Marino et al.   SCIENCE

Systematic discovery of natural CRISPR-Cas12a inhibitors

(2018) Kyle E. Watters et al.   SCIENCE

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins

(2018) Jooyoung Lee et al.   mBio

Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28

(2017) Aaron A. Smargon et al.   MOLECULAR CELL

Anti-CRISPR: discovery, mechanism and function

(2017) April Pawluk et al.   NATURE REVIEWS MICROBIOLOGY

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

The Discovery, Mechanisms, and Evolutionary Impact of Anti-CRISPRs

(2017) Adair L. Borges et al.   Annual Review of Virology

DISARM is a widespread bacterial defence system with broad anti-phage activities

(2017) Gal Ofir et al.   Nature Microbiology

An anti-CRISPR from a virulent streptococcal phage inhibits Streptococcus pyogenes Cas9

(2017) Alexander P. Hynes et al.   Nature Microbiology

Naturally Occurring Off-Switches for CRISPR-Cas9

(2016) April Pawluk et al.   CELL

Genetic and life-history traits associated with the distribution of prophages in bacteria

(2016) Marie Touchon et al.   ISME Journal

Molecular Mechanisms of Innate Immune Inhibition by Non-Segmented Negative-Sense RNA Viruses

(2016) Srirupa Chatterjee et al.   JOURNAL OF MOLECULAR BIOLOGY

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

(2016) Adrian G. Patterson et al.   MOLECULAR CELL

The diversity-generating benefits of a prokaryotic adaptive immune system

(2016) Stineke van Houte et al.   NATURE

Quorum sensing controls thePseudomonas aeruginosaCRISPR-Cas adaptive immune system

(2016) Nina M. Høyland-Kroghsbo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Manipulating Bacterial Communities by in situ Microbiome Engineering

(2016) Ravi U. Sheth et al.   TRENDS IN GENETICS

Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species

(2016) April Pawluk et al.   Nature Microbiology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Parasite Exposure Drives Selective Evolution of Constitutive versus Inducible Defense

(2015) Edze R. Westra et al.   CURRENT BIOLOGY

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery

(2015) Daria Vorontsova et al.   NUCLEIC ACIDS RESEARCH

Regulation of the Type I-F CRISPR-Cas system by CRP-cAMP and GalM controls spacer acquisition and interference

(2015) Adrian G. Patterson et al.   NUCLEIC ACIDS RESEARCH

Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9

(2015) Alexandra L. Bryson et al.   mBio

Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus

(2014) Gintautas Tamulaitis et al.   MOLECULAR CELL

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A New Group of Phage Anti-CRISPR Genes Inhibits the Type I-E CRISPR-Cas System of Pseudomonas aeruginosa

(2014) A. Pawluk et al.   mBio

crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus

(2013) Tautvydas Karvelis et al.   RNA Biology

CRISPR-Cas systems target a diverse collection of invasive mobile genetic elements in human microbiomes

(2013) Quan Zhang et al.   GENOME BIOLOGY

Rules of Engagement: Molecular Insights from Host-Virus Arms Races

(2012) Matthew D. Daugherty et al.   Annual Review of Genetics

Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system

(2012) Joe Bondy-Denomy et al.   NATURE

Exploring the DNA mimicry of the Ocr protein of phage T7

(2012) Gareth A. Roberts et al.   NUCLEIC ACIDS RESEARCH

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Bacteriophages and their genomes

(2011) Graham F Hatfull et al.   Current Opinion in Virology

Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli

(2010) Ritsdeliz Perez-Rodriguez et al.   MOLECULAR MICROBIOLOGY

Identification and characterization ofE. coliCRISPR-caspromoters and their silencing by H-NS

(2010) Ümit Pul et al.   MOLECULAR MICROBIOLOGY

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE