Marker-free co-selection for successive rounds of prime editing in human cells

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

(2022) J. Ferreira da Silva et al.   Nature Communications

Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing

(2022) Martin Peterka et al.   Nature Communications

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

(2022) Dorottya Anna Simon et al.   eLife

Designing and executing prime editing experiments in mammalian cells

(2022) Jordan L. Doman et al.   Nature Protocols

Universal toxin-based selection for precise genome engineering in human cells

(2021) Songyuan Li et al.   Nature Communications

Imaging dynamic mTORC1 pathway activity in vivo reveals marked shifts that support time-specific inhibitor therapy in AML

(2021) Toshihiko Oki et al.   Nature Communications

PrimeDesign software for rapid and simplified design of prime editing guide RNAs

(2021) Jonathan Y. Hsu et al.   Nature Communications

Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger Sequencing Data

(2021) Kevin Bloh et al.   CRISPR Journal

CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells

(2021) Karl Petri et al.   NATURE BIOTECHNOLOGY

Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice

(2021) Pengpeng Liu et al.   Nature Communications

Role of 53BP1 in end protection and DNA synthesis at DNA breaks

(2021) Jacob Paiano et al.   GENES & DEVELOPMENT

Optimized nickase- and nuclease-based prime editing in human and mouse cells

(2021) Fatwa Adikusuma et al.   NUCLEIC ACIDS RESEARCH

Small tandem DNA duplications result from CST-guided Pol α-primase action at DNA break termini

(2021) Joost Schimmel et al.   Nature Communications

RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit

(2021) Emilie L. Cerezo et al.   PLoS Genetics

Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases

(2021) Hyewon Jang et al.   Nature Biomedical Engineering

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

Overlapping genes in natural and engineered genomes

(2021) Bradley W. Wright et al.   NATURE REVIEWS GENETICS

53BP1: a DSB escort

(2020) Zachary Mirman et al.   GENES & DEVELOPMENT

Prime Editing: Precision Genome Editing by Reverse Transcription

(2020) Jun Yan et al.   MOLECULAR CELL

Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation

(2020) Assala Lamaa et al.   eLife

Separase-triggered apoptosis enforces minimal length of mitosis

(2020) Susanne Hellmuth et al.   NATURE

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

(2020) Duran Sürün et al.   Genes

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Metabolic engineering generates a transgene-free safety switch for cell therapy

(2020) Volker Wiebking et al.   NATURE BIOTECHNOLOGY

Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation

(2020) Thomas L. Maurissen et al.   Nature Communications

Predicting the efficiency of prime editing guide RNAs in human cells

(2020) Hui Kwon Kim et al.   NATURE BIOTECHNOLOGY

The molecular basis and disease relevance of non-homologous DNA end joining

(2020) Bailin Zhao et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Enteroviral 3C protease activates the human NLRP1 inflammasome in airway epithelia

(2020) Kim S. Robinson et al.   SCIENCE

Prime editing for functional repair in patient-derived disease models

(2020) Imre F. Schene et al.   Nature Communications

Genome editing retraces the evolution of toxin resistance in the monarch butterfly

(2019) Marianthi Karageorgi et al.   NATURE

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

A unified allosteric/torpedo mechanism for transcriptional termination on human protein-coding genes

(2019) Joshua D. Eaton et al.   GENES & DEVELOPMENT

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

Strategies for the Enrichment and Selection of Genetically Modified Cells

(2018) Chonghua Ren et al.   TRENDS IN BIOTECHNOLOGY

CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons

(2017) Pierre Billon et al.   MOLECULAR CELL

Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing efficiency

(2017) Marella D Canny et al.   NATURE BIOTECHNOLOGY

Marker-free coselection for CRISPR-driven genome editing in human cells

(2017) Daniel Agudelo et al.   NATURE METHODS

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

(2017) Anne Bothmer et al.   Nature Communications

Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool

(2017) Katherine F. Donovan et al.   PLoS One

Overcoming mTOR resistance mutations with a new-generation mTOR inhibitor

(2016) Vanessa S. Rodrik-Outmezguine et al.   NATURE

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

CRISPR-Mediated Drug-Target Validation Reveals Selective Pharmacological Inhibition of the RNA Helicase, eIF4A

(2016) Jennifer Chu et al.   Cell Reports

Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion

(2016) D. T. Ge et al.   G3-Genes Genomes Genetics

Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy

(2016) Nanci S. Kane et al.   G3-Genes Genomes Genetics

Structures and characterization of digoxin- and bufalin-bound Na+,K+-ATPase compared with the ouabain-bound complex

(2015) Mette Laursen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Widespread convergence in toxin resistance by predictable molecular evolution

(2015) Beata Ujvari et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells

(2015) Mathieu Dalvai et al.   Cell Reports

A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans

(2014) Heesun Kim et al.   GENETICS

Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans

(2014) J. A. Arribere et al.   GENETICS

Response and Acquired Resistance to Everolimus in Anaplastic Thyroid Cancer

(2014) Nikhil Wagle et al.   NEW ENGLAND JOURNAL OF MEDICINE

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

A Diverse Array of Cancer-Associated MTOR Mutations Are Hyperactivating and Can Predict Rapamycin Sensitivity

(2014) B. C. Grabiner et al.   Cancer Discovery

Activating mTOR Mutations in a Patient with an Extraordinary Response on a Phase I Trial of Everolimus and Pazopanib

(2014) N. Wagle et al.   Cancer Discovery

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Cancer translocations in human cells induced by zinc finger and TALE nucleases

(2013) M. Piganeau et al.   GENOME RESEARCH

Mutations in Critical Domains Confer the HumanmTORGene Strong Tumorigenicity

(2013) Avaniyapuram Kannan Murugan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal structure of the high-affinity Na+,K+-ATPase-ouabain complex with Mg2+ bound in the cation binding site

(2013) M. Laursen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RPA Accumulation during Class Switch Recombination Represents 5′–3′ DNA-End Resection during the S–G2/M Phase of the Cell Cycle

(2013) Arito Yamane et al.   Cell Reports

Intratumor Heterogeneity and Branched Evolution Revealed by Multiregion Sequencing

(2012) Marco Gerlinger et al.   NEW ENGLAND JOURNAL OF MEDICINE

Parallel Molecular Evolution in an Herbivore Community

(2012) Y. Zhen et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

AZD8055 Is a Potent, Selective, and Orally Bioavailable ATP-Competitive Mammalian Target of Rapamycin Kinase Inhibitor with In vitro and In vivo Antitumor Activity

(2009) C. M. Chresta et al.   CANCER RESEARCH

Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain

(2009) H. Ogawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isolation of Hyperactive Mutants of Mammalian Target of Rapamycin

(2008) Yoichiro Ohne et al.   JOURNAL OF BIOLOGICAL CHEMISTRY