Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures

SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress

(2022) Katharina M. Scherer et al.   Science Advances

Secondary structural ensembles of the SARS-CoV-2 RNA genome in infected cells

(2022) Tammy C. T. Lan et al.   Nature Communications

A conceptual framework for understanding phase separation and addressing open questions and challenges

(2022) Tanja Mittag et al.   MOLECULAR CELL

Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions

(2022) Mrityunjoy Kar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comprehensive in vivo secondary structure of the SARS-CoV-2 genome reveals novel regulatory motifs and mechanisms

(2021) Nicholas C. Huston et al.   MOLECULAR CELL

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

(2021) Shan Lu et al.   Nature Communications

SARS-CoV-2 nucleocapsid protein undergoes liquid–liquid phase separation into stress granules through its N-terminal intrinsically disordered region

(2021) Jia Wang et al.   Cell Discovery

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs

(2021) Lei Sun et al.   CELL

Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions

(2021) Georg Krainer et al.   Nature Communications

In vivo reconstitution finds multivalent RNA-RNA interactions as drivers of mesh-like condensates

(2021) Weirui Ma et al.   eLife

Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA

(2021) Heather M. Forsythe et al.   BIOPHYSICAL JOURNAL

GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein

(2021) Ming Zhao et al.   Nature Communications

The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA

(2021) Jasmine Cubuk et al.   Nature Communications

Energetic and structural features of SARS-CoV-2 N-protein co-assemblies with nucleic acids

(2021) Huaying Zhao et al.   iScience

Dynamics of the SARS-CoV-2 nucleoprotein N-terminal domain triggers RNA duplex destabilization

(2021) Ícaro P. Caruso et al.   BIOPHYSICAL JOURNAL

Role of spatial patterning of N-protein interactions in SARS-CoV-2 genome packaging

(2021) Ian Seim et al.   BIOPHYSICAL JOURNAL

The architecture of the SARS-CoV-2 RNA genome inside virion

(2021) Changchang Cao et al.   Nature Communications

Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains

(2021) Anne Bremer et al.   Nature Chemistry

Rapid assessment of SARS-CoV-2–evolved variants using virus-like particles

(2021) Abdullah M. Syed et al.   SCIENCE

SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA

(2021) Amanda Jack et al.   PLOS BIOLOGY

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

(2020) Erik W. Martin et al.   SCIENCE

The Architecture of SARS-CoV-2 Transcriptome

(2020) Dongwan Kim et al.   CELL

Quantifying the heterogeneity of macromolecular machines by mass photometry

(2020) Adar Sonn-Segev et al.   Nature Communications

Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking

(2020) Anne Monette et al.   Cell Reports

SARS-CoV-2 (COVID-19) by the numbers

(2020) Yinon M Bar-On et al.   eLife

Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly

(2020) Serafima Guseva et al.   Science Advances

Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites

(2020) Sisi Kang et al.   Acta Pharmaceutica Sinica B

Biochemical characterization of SARS-CoV-2 nucleocapsid protein

(2020) Weihong Zeng et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A proposed role for the SARS‐CoV‐2 nucleocapsid protein in the formation and regulation of biomolecular condensates

(2020) Sean M. Cascarina et al.   FASEB JOURNAL

RNA contributions to the form and function of biomolecular condensates

(2020) Christine Roden et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2

(2020) Luca Zinzula et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Molecular Architecture of the SARS-CoV-2 Virus

(2020) Hangping Yao et al.   CELL

Liquid–liquid phase separation by SARS-CoV-2 nucleocapsid protein and RNA

(2020) Hui Chen et al.   CELL RESEARCH

The coding capacity of SARS-CoV-2

(2020) Yaara Finkel et al.   NATURE

Analysis of RNA–protein networks with RNP-MaP defines functional hubs on RNA

(2020) Chase A. Weidmann et al.   NATURE BIOTECHNOLOGY

SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

(2020) Theodora Myrto Perdikari et al.   EMBO JOURNAL

Liquid–Liquid Phase Separation by Intrinsically Disordered Protein Regions of Viruses: Roles in Viral Life Cycle and Control of Virus–Host Interactions

(2020) Stefania Brocca et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions

(2020) Christopher R. Carlson et al.   MOLECULAR CELL

Nonstructural Protein 1 of SARS-CoV-2 Is a Potent Pathogenicity Factor Redirecting Host Protein Synthesis Machinery toward Viral RNA

(2020) Shuai Yuan et al.   MOLECULAR CELL

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

(2020) Christiane Iserman et al.   MOLECULAR CELL

The viral protein NSP1 acts as a ribosome gatekeeper for shutting down host translation and fostering SARS-CoV-2 translation

(2020) Antonin TIDU et al.   RNA

Protein condensates as aging Maxwell fluids

(2020) Louise Jawerth et al.   SCIENCE

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

(2020) Adriana Savastano et al.   Nature Communications

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

(2020) Steffen Klein et al.   Nature Communications

Structure and oligomerization state of the C-terminal region of the Middle East respiratory syndrome coronavirus nucleoprotein

(2019) Thi Hong Van Nguyen et al.   Acta Crystallographica Section D-Structural Biology

Phosphoregulated FMRP phase separation models activity-dependent translation through bidirectional control of mRNA granule formation

(2019) Brian Tsang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

(2019) Marta Alenquer et al.   Nature Communications

Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor

(2019) Ellen C. O’Shaughnessy et al.   JOURNAL OF CELL BIOLOGY

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

LASSI: A lattice model for simulating phase transitions of multivalent proteins

(2019) Jeong-Mo Choi et al.   PLoS Computational Biology

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Post-translational modifications of coronavirus proteins: roles and function

(2018) To Sing Fung et al.   Future Virology

mRNA structure determines specificity of a polyQ-driven phase separation

(2018) Erin M. Langdon et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Pi-Pi contacts are an overlooked protein feature relevant to phase separation

(2018) Robert McCoy Vernon et al.   eLife

Phase Transitions Drive the Formation of Vesicular Stomatitis Virus Replication Compartments

(2018) Bianca S. Heinrich et al.   mBio

Accurate detection of chemical modifications in RNA by mutational profiling (MaP) with ShapeMapper 2

(2017) Steven Busan et al.   RNA

Negri bodies are viral factories with properties of liquid organelles

(2017) Jovan Nikolic et al.   Nature Communications

Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus

(2017) Vincent Rincheval et al.   Nature Communications

RNA Controls PolyQ Protein Phase Transitions

(2015) Huaiying Zhang et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Selective 2′-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurate RNA structure analysis

(2015) Matthew J Smola et al.   Nature Protocols

The structure and functions of coronavirus genomic 3′ and 5′ ends

(2015) Dong Yang et al.   VIRUS RESEARCH

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

The SARS coronavirus nucleocapsid protein – Forms and functions

(2014) Chung-ke Chang et al.   ANTIVIRAL RESEARCH

The Coronavirus Nucleocapsid Is a Multifunctional Protein

(2014) Ruth McBride et al.   Viruses-Basel

Mouse Hepatitis Virus Stem-Loop 4 Functions as a Spacer Element Required To Drive Subgenomic RNA Synthesis

(2011) D. Yang et al.   JOURNAL OF VIROLOGY

An Optimal cis-Replication Stem-Loop IV in the 5' Untranslated Region of the Mouse Coronavirus Genome Extends 16 Nucleotides into Open Reading Frame 1

(2011) B.-J. Guan et al.   JOURNAL OF VIROLOGY

Coronavirus N Protein N-Terminal Domain (NTD) Specifically Binds the Transcriptional Regulatory Sequence (TRS) and Melts TRS-cTRS RNA Duplexes

(2009) Nicholas E. Grossoehme et al.   JOURNAL OF MOLECULAR BIOLOGY

Glycogen Synthase Kinase-3 Regulates the Phosphorylation of Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein and Viral Replication

(2008) Chia-Hsin Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Lability in Stem–Loop 1 Drives a 5′ UTR–3′ UTR Interaction in Coronavirus Replication

(2008) Lichun Li et al.   JOURNAL OF MOLECULAR BIOLOGY

Solution Structure of the C-terminal Dimerization Domain of SARS Coronavirus Nucleocapsid Protein Solved by the SAIL-NMR Method

(2007) Mitsuhiro Takeda et al.   JOURNAL OF MOLECULAR BIOLOGY