Repurposing CRISPR RNA-guided integrases system for one-step, efficient genomic integration of ultra-long DNA sequences

Structural basis for target site selection in RNA-guided DNA transposition systems

(2021) Jung-Un Park et al.   SCIENCE

Developing a base‐editing system to expand the carbon source utilization spectra of Shewanella oneidensis MR‐1 for enhanced pollutant degradation

(2020) Lei Cheng et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

(2020) Phuc Leo H. Vo et al.   NATURE BIOTECHNOLOGY

Synthetic biology approaches for chromosomal integration of genes and pathways in industrial microbial systems

(2019) Lei Li et al.   BIOTECHNOLOGY ADVANCES

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

(2019) D. Dewran Kocak et al.   NATURE BIOTECHNOLOGY

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

(2019) Sanne E. Klompe et al.   NATURE

Jumping at the chance for precise DNA integration

(2019) Jennifer B. Kwon et al.   NATURE BIOTECHNOLOGY

RNA-guided DNA insertion with CRISPR-associated transposases

(2019) Jonathan Strecker et al.   SCIENCE

Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance

(2019) Tanita Wein et al.   Nature Communications

Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

(2019) José M. Pastor et al.   Microbial Cell Factories

A Broad-Host-Range CRISPRi Toolkit for Silencing Gene Expression in Burkholderia

(2019) Andrew M. Hogan et al.   ACS Synthetic Biology

Mapping the Genetic Landscape of Human Cells

(2018) Max A. Horlbeck et al.   CELL

Synthetic auxotrophs for stable and tunable maintenance of plasmid copy number

(2018) Chae Won Kang et al.   METABOLIC ENGINEERING

Engineering sucrose metabolism in Pseudomonas putida highlights the importance of porins

(2018) Hannes Löwe et al.   Microbial Biotechnology

Modular engineering to increase intracellular NAD(H/+) promotes rate of extracellular electron transfer of Shewanella oneidensis

(2018) Feng Li et al.   Nature Communications

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi

(2018) Jason M. Peters et al.   Nature Microbiology

aMSGE: advanced multiplex site-specific genome engineering with orthogonal modular recombinases in actinomycetes

(2018) Lei Li et al.   METABOLIC ENGINEERING

Enhancing Extracellular Electron Transfer of Shewanella oneidensis MR-1 through Coupling Improved Flavin Synthesis and Metal-Reducing Conduit for Pollutant Degradation

(2017) Di Min et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

Microbial α-amylase: A biomolecular overview

(2017) Qiaoge Zhang et al.   PROCESS BIOCHEMISTRY

Cell Factory Engineering

(2017) Anne Mathilde Davy et al.   Cell Systems

Enhanced integration of large DNA intoE. colichromosome by CRISPR/Cas9

(2016) Mu-En Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

DNA Transposition at Work

(2016) Alison B. Hickman et al.   CHEMICAL REVIEWS

Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis

(2016) Yi Xiao et al.   Nature Chemical Biology

Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii as an Example

(2016) Yi Wang et al.   ACS Synthetic Biology

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

DNA library construction using Gibson Assembly®

(2015) Steven Thomas et al.   NATURE METHODS

A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies

(2015) Pengfei Gu et al.   Scientific Reports

High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

(2014) Junjie Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis

(2014) Peter J Enyeart et al.   Mobile DNA

Thermal Stability and Starch Degradation Profile of α-Amylase fromStreptomyces avermitilis

(2013) Sang Youn HWANG et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

(2013) Suriana Sabri et al.   Microbial Cell Factories

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Flavin Electron Shuttles Dominate Extracellular Electron Transfer by Shewanella oneidensis

(2013) N. J. Kotloski et al.   mBio

Implementation of stable and complex biological systems through recombinase-assisted genome engineering

(2013) Christine Nicole S. Santos et al.   Nature Communications

The future of metabolic engineering and synthetic biology: Towards a systematic practice

(2012) Vikramaditya G. Yadav et al.   METABOLIC ENGINEERING

A decade’s perspective on DNA sequencing technology

(2011) Elaine R. Mardis   NATURE

Marker-free plasmids for gene therapeutic applications—Lack of antibiotic resistance gene substantially improves the manufacturing process

(2010) Jürgen Mairhofer et al.   JOURNAL OF BIOTECHNOLOGY