The biofilm life cycle: expanding the conceptual model of biofilm formation

Bacterial biofilms predominate in both acute and chronic human lung infections

(2022) Mette Kolpen et al.   THORAX

Who put the film in biofilm? The migration of a term from wastewater engineering to medicine and beyond

(2021) Hans-Curt Flemming et al.   npj Biofilms and Microbiomes

Human organoid biofilm model for assessing antibiofilm activity of novel agents

(2021) Bing (Catherine) Wu et al.   npj Biofilms and Microbiomes

A Heterogeneously Expressed Gene Family Modulates the Biofilm Architecture and Hypoxic Growth of Aspergillus fumigatus

(2021) Caitlin H. Kowalski et al.   mBio

Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections

(2021) Erin S. Gloag et al.   Scientific Reports

Establishing the Root Cause of a Failure in a Firewater Pipeline

(2021) Abbas Bahrami et al.   ENGINEERING FAILURE ANALYSIS

Staphylococcus aureus Floating Biofilm Formation and Phenotype in Synovial Fluid Depends on Albumin, Fibrinogen, and Hyaluronic Acid

(2021) Samantha Knott et al.   Frontiers in Microbiology

The impact of mental models on the treatment and research of chronic infections due to biofilms

(2021) Thomas Bjarnsholt et al.   APMIS

Development of microalgal biofilm for wastewater remediation: From mechanism to practical application

(2021) Yingbing Hu et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

The importance of understanding the infectious microenvironment

(2021) Thomas Bjarnsholt et al.   LANCET INFECTIOUS DISEASES

Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution

(2021) Daniel Dar et al.   SCIENCE

Interactions between periphytic biofilms and dissolved organic matter at soil-water interface and the consequent effects on soil phosphorus fraction changes

(2021) Junzhuo Liu et al.   SCIENCE OF THE TOTAL ENVIRONMENT

The impact of bacterial cell aggregation on UV inactivation kinetics

(2021) Eirini Vitzilaiou et al.   WATER RESEARCH

Spatial alanine metabolism determines local growth dynamics of Escherichia coli colonies

(2021) Francisco Díaz-Pascual et al.   eLife

Flow and diffusion around and within diatom aggregates: Effects of aggregate composition and shape

(2020) Eva‐Maria Zetsche et al.   LIMNOLOGY AND OCEANOGRAPHY

Universal Dermal Microbiome in Human Skin

(2020) Lene Bay et al.   mBio

Quantitative Framework for Model Evaluation in Microbiology Research Using Pseudomonas aeruginosa and Cystic Fibrosis Infection as a Test Case

(2020) Daniel M. Cornforth et al.   mBio

Granular biofilms: Function, application, and new trends as model microbial communities

(2020) Anna Christine Trego et al.   CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY

Investigation of synovial fluid induced Staphylococcus aureus aggregate development and its impact on surface attachment and biofilm formation

(2020) Matthew J. Pestrak et al.   PLoS One

Spatial mapping of polymicrobial communities reveals a precise biogeography associated with human dental caries

(2020) Dongyeop Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interactions between dicyandiamide and periphytic biofilms in paddy soils and subsequent effects on nitrogen cycling

(2020) Yanhui Zhao et al.   SCIENCE OF THE TOTAL ENVIRONMENT

The extracellular matrix protein TasA is a developmental cue that maintains a motile subpopulation within Bacillus subtilis biofilms

(2020) Nitai Steinberg et al.   Science Signaling

Biofilm dispersion

(2020) Kendra P. Rumbaugh et al.   NATURE REVIEWS MICROBIOLOGY

The zone model: A conceptual model for understanding the microenvironment of chronic wound infection

(2020) Klaus Kirketerp‐Møller et al.   WOUND REPAIR AND REGENERATION

Identification and Characterization of Planktonic Biofilm-Like Aggregates in Infected Synovial Fluids From Joint Infections

(2020) Alessandro Bidossi et al.   Frontiers in Microbiology

Current In Vitro Biofilm-Infected Chronic Wound Models for Developing New Treatment Possibilities

(2020) Ida C. Thaarup et al.   Advances in Wound Care

Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes

(2020) Maria Alhede et al.   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

Fungal biofilm architecture produces hypoxic microenvironments that drive antifungal resistance

(2020) Caitlin H. Kowalski et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Metabolic Microenvironments Drive Microbial Differentiation and Antibiotic Resistance

(2020) Tatyana L. Povolotsky et al.   TRENDS IN GENETICS

Long-term souring treatment using nitrate and biocides in high-temperature oil reservoirs

(2020) Diogo Jurelevicius et al.   FUEL

Bacteria and archaea on Earth and their abundance in biofilms

(2019) Hans-Curt Flemming et al.   NATURE REVIEWS MICROBIOLOGY

Biofilms: The Microbial “Protective Clothing” in Extreme Environments

(2019) Wen Yin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Equine or porcine synovial fluid as a novel ex vivo model for the study of bacterial free-floating biofilms that form in human joint infections

(2019) Jessica M. Gilbertie et al.   PLoS One

Emergent microscale gradients give rise to metabolic cross-feeding and antibiotic tolerance in clonal bacterial populations

(2019) Alma Dal Co et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Impact of environmental biofilms: Industrial components and its remediation

(2019) Vinita Vishwakarma   JOURNAL OF BASIC MICROBIOLOGY

The mechanisms of granulation of activated sludge in wastewater treatment, its optimization, and impact on effluent quality

(2018) Britt-Marie Wilén et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Stability of aerobic granules during long-term bioreactor operation

(2018) Rita D.G. Franca et al.   BIOTECHNOLOGY ADVANCES

Microbial mat ecosystems: Structure types, functional diversity, and biotechnological application

(2018) Cristina M. Prieto-Barajas et al.   ELECTRONIC JOURNAL OF BIOTECHNOLOGY

Anaerobic microbiologically influenced corrosion mechanisms interpreted using bioenergetics and bioelectrochemistry: A review

(2018) Yingchao Li et al.   JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY

Functional biogeography and host specificity of bacterial communities associated with the Marine Green Alga Ulva spp.

(2018) Alexandra J. Roth-Schulze et al.   MOLECULAR ECOLOGY

Pseudomonas aeruginosatranscriptome during human infection

(2018) Daniel M. Cornforth et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Oral Biofilms: Pathogens, Matrix, and Polymicrobial Interactions in Microenvironments

(2018) William H. Bowen et al.   TRENDS IN MICROBIOLOGY

Validation of biofilm formation on human skin wound models and demonstration of clinically translatable bacteria-specific volatile signatures

(2018) Mohammed Ashrafi et al.   Scientific Reports

Synergistic Interactions within a Multispecies Biofilm Enhance Individual Species Protection against Grazing by a Pelagic Protozoan

(2018) Prem K. Raghupathi et al.   Frontiers in Microbiology

Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds

(2018) Lene Bay et al.   Advances in Wound Care

Sonication versus tissue sampling for diagnosis of prosthetic joint and other orthopaedic device-related infections.

(2018) Maria Dudareva et al.   JOURNAL OF CLINICAL MICROBIOLOGY

High-resolution in situ transcriptomics of Pseudomonas aeruginosa unveils genotype independent patho-phenotypes in cystic fibrosis lungs

(2018) Elio Rossi et al.   Nature Communications

Spatial organization of different sigma factor activities and c-di-GMP signalling within the three-dimensional landscape of a bacterial biofilm

(2018) Gisela Klauck et al.   Open Biology

Influence of Dental Prosthesis and Restorative Materials Interface on Oral Biofilms

(2018) Yu Hao et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Entropically driven aggregation of bacteria by host polymers promotes antibiotic tolerance in Pseudomonas aeruginosa

(2018) Patrick R. Secor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microalgal biofilms: A further step over current microalgal cultivation techniques

(2018) Antonia Mantzorou et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Divide and conquer: the P seudomonas aeruginosa two-component hybrid SagS enables biofilm formation and recalcitrance of biofilm cells to antimicrobial agents via distinct regulatory circuits

(2017) Olga E. Petrova et al.   ENVIRONMENTAL MICROBIOLOGY

Low-abundant species facilitates specific spatial organization that promotes multispecies biofilm formation

(2017) Wenzheng Liu et al.   ENVIRONMENTAL MICROBIOLOGY

Fluid-driven interfacial instabilities and turbulence in bacterial biofilms

(2017) Stefania Fabbri et al.   ENVIRONMENTAL MICROBIOLOGY

Primary ciliary dyskinesia ciliated airway cells show increased susceptibility to Haemophilus influenzae biofilm formation

(2017) Woolf T. Walker et al.   EUROPEAN RESPIRATORY JOURNAL

Novel porcine model of implant-associated osteomyelitis: A comprehensive analysis of local, regional, and systemic response

(2017) Louise Kruse Jensen et al.   JOURNAL OF ORTHOPAEDIC RESEARCH

Staphylococcus aureus biofilm: a complex developmental organism

(2017) Derek E. Moormeier et al.   MOLECULAR MICROBIOLOGY

Spatial transcriptomes within the Pseudomonas aeruginosa biofilm architecture

(2017) Yun Heacock-Kang et al.   MOLECULAR MICROBIOLOGY

Targeting microbial biofilms: current and prospective therapeutic strategies

(2017) Hyun Koo et al.   NATURE REVIEWS MICROBIOLOGY

Cyclic di-GMP: second messenger extraordinaire

(2017) Urs Jenal et al.   NATURE REVIEWS MICROBIOLOGY

The role of hydrodynamics in shaping the composition and architecture of epilithic biofilms in fluvial ecosystems

(2017) Ute Risse-Buhl et al.   WATER RESEARCH

The Follicular Skin Microbiome in Patients With Hidradenitis Suppurativa and Healthy Controls

(2017) Hans Christian Ring et al.   JAMA Dermatology

Critical review on biofilm methods

(2016) Joana Azeredo et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Escaping the biofilm in more than one way: desorption, detachment or dispersion

(2016) Olga E Petrova et al.   CURRENT OPINION IN MICROBIOLOGY

Real Time, Spatial, and Temporal Mapping of the Distribution of c-di-GMP during Biofilm Development

(2016) Harikrishnan A. S. Nair et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biofilms and Cyclic di-GMP (c-di-GMP) Signaling: Lessons fromPseudomonas aeruginosaand Other Bacteria

(2016) Martina Valentini et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Role of Multicellular Aggregates in Biofilm Formation

(2016) Kasper N. Kragh et al.   mBio

The Architecture of Iron Microbial Mats Reflects the Adaptation of Chemolithotrophic Iron Oxidation in Freshwater and Marine Environments

(2016) Clara S. Chan et al.   Frontiers in Microbiology

Staphylococcal Persistence Due to Biofilm Formation in Synovial Fluid Containing Prophylactic Cefazolin

(2015) Sana S. Dastgheyb et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review

(2015) Youry Pii et al.   BIOLOGY AND FERTILITY OF SOILS

ESCMID∗ guideline for the diagnosis and treatment of biofilm infections 2014

(2015) N. Høiby et al.   CLINICAL MICROBIOLOGY AND INFECTION

Chronic pulmonary disease withMycobacterium abscessuscomplex is a biofilm infection

(2015) Tavs Qvist et al.   EUROPEAN RESPIRATORY JOURNAL

Stress responses go three dimensional - the spatial order of physiological differentiation in bacterial macrocolony biofilms

(2014) Diego O. Serra et al.   ENVIRONMENTAL MICROBIOLOGY

Effect of Biofilms on Recalcitrance of Staphylococcal Joint Infection to Antibiotic Treatment

(2014) S. Dastgheyb et al.   JOURNAL OF INFECTIOUS DISEASES

Biofilm development and enhanced stress resistance of a model, mixed-species community biofilm

(2013) Kai Wei Kelvin Lee et al.   ISME Journal

Biofilms 2012: New Discoveries and Significant Wrinkles in a Dynamic Field

(2013) S. Haussler et al.   JOURNAL OF BACTERIOLOGY

Antimicrobial Tolerance of Pseudomonas aeruginosa Biofilms Is Activated during an Early Developmental Stage and Requires the Two-Component Hybrid SagS

(2013) K. Gupta et al.   JOURNAL OF BACTERIOLOGY

The MerR-Like Regulator BrlR Confers Biofilm Tolerance by Activating Multidrug Efflux Pumps in Pseudomonas aeruginosa Biofilms

(2013) J. Liao et al.   JOURNAL OF BACTERIOLOGY

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

(2013) U. Romling et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Sticking together: building a biofilm the Bacillus subtilis way

(2013) Hera Vlamakis et al.   NATURE REVIEWS MICROBIOLOGY

Whole Transcriptome Analysis of Acinetobacter baumannii Assessed by RNA-Sequencing Reveals Different mRNA Expression Profiles in Biofilm Compared to Planktonic Cells

(2013) Soraya Rumbo-Feal et al.   PLoS One

The in vivo biofilm

(2013) Thomas Bjarnsholt et al.   TRENDS IN MICROBIOLOGY

Towards diagnostic guidelines for biofilm-associated infections

(2012) Luanne Hall-Stoodley et al.   FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY

Heterogeneity in Pseudomonas aeruginosa Biofilms Includes Expression of Ribosome Hibernation Factors in the Antibiotic-Tolerant Subpopulation and Hypoxia-Induced Stress Response in the Metabolically Active Population

(2012) K. S. Williamson et al.   JOURNAL OF BACTERIOLOGY

Aggregation by depletion attraction in cultures of bacteria producing exopolysaccharide

(2012) G. Dorken et al.   Journal of the Royal Society Interface

Microcolony formation by the opportunistic pathogenPseudomonas aeruginosarequires pyruvate and pyruvate fermentation

(2012) Olga E. Petrova et al.   MOLECULAR MICROBIOLOGY

Bacterial biofilms in the upper airway - evidence for role in pathology and implications for treatment of otitis media

(2012) Lauren O. Bakaletz   Paediatric Respiratory Reviews

Planktonic Aggregates of Staphylococcus aureus Protect against Common Antibiotics

(2012) Jakob Haaber et al.   PLoS One

Self-produced exopolysaccharide is a signal that stimulates biofilm formation in Pseudomonas aeruginosa

(2012) Y. Irie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Small RNAs and their role in biofilm formation

(2012) Jacob R. Chambers et al.   TRENDS IN MICROBIOLOGY

The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix

(2011) Kelly M. Colvin et al.   ENVIRONMENTAL MICROBIOLOGY

SagS Contributes to the Motile-Sessile Switch and Acts in Concert with BfiSR To Enable Pseudomonas aeruginosa Biofilm Formation

(2011) O. E. Petrova et al.   JOURNAL OF BACTERIOLOGY

The novel Pseudomonas aeruginosa two-component regulator BfmR controls bacteriophage-mediated lysis and DNA release during biofilm development through PhdA

(2011) Olga E. Petrova et al.   MOLECULAR MICROBIOLOGY

In-Vivo Expression Profiling of Pseudomonas aeruginosa Infections Reveals Niche-Specific and Strain-Independent Transcriptional Programs

(2011) Piotr Bielecki et al.   PLoS One

Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm

(2011) Morten Alhede et al.   PLoS One

Physiology of Pseudomonas aeruginosa in biofilms as revealed by transcriptome analysis

(2010) James P Folsom et al.   BMC MICROBIOLOGY

Biofilms in chronic infections – a matter of opportunity – monospecies biofilms in multispecies infections

(2010) Mette Burmølle et al.   FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY

The Novel Two-Component Regulatory System BfiSR Regulates Biofilm Development by Controlling the Small RNA rsmZ through CafA

(2010) O. E. Petrova et al.   JOURNAL OF BACTERIOLOGY

Vibrio cholerae VpsT Regulates Matrix Production and Motility by Directly Sensing Cyclic di-GMP

(2010) P. V. Krasteva et al.   SCIENCE

Effect of a solution containing citrate/Methylene Blue/parabens on Staphylococcus aureus bacteria and biofilm, and comparison with various heparin solutions

(2009) K. Sauer et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Pseudomonas aeruginosabiofilms in the respiratory tract of cystic fibrosis patients

(2009) Thomas Bjarnsholt et al.   PEDIATRIC PULMONOLOGY

Pseudomonas aeruginosa PAO1 Preferentially Grows as Aggregates in Liquid Batch Cultures and Disperses upon Starvation

(2009) David Schleheck et al.   PLoS One

The developmental model of microbial biofilms: ten years of a paradigm up for review

(2009) Russell D. Monds et al.   TRENDS IN MICROBIOLOGY

A Novel Signaling Network Essential for Regulating Pseudomonas aeruginosa Biofilm Development

(2009) Olga E. Petrova et al.   PLoS Pathogens

Localized Gene Expression in Pseudomonas aeruginosa Biofilms

(2008) A. P. Lenz et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates

(2008) Luanne Hall-Stoodley et al.   BMC MICROBIOLOGY

Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy

(2008) Sünje Johanna Pamp et al.   CYTOMETRY PART A

Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures inPseudomonas aeruginosabiofilms

(2008) Kim B. Barken et al.   ENVIRONMENTAL MICROBIOLOGY

Causes and consequences of plant-associated biofilms

(2008) Thimmaraju Rudrappa et al.   FEMS MICROBIOLOGY ECOLOGY

Distribution, Organization, and Ecology of Bacteria in Chronic Wounds

(2008) K. Kirketerp-Moller et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Tolerance to the antimicrobial peptide colistin in Pseudomonas aeruginosa biofilms is linked to metabolically active cells, and depends on the pmr and mexAB-oprM genes

(2008) Sünje Johanna Pamp et al.   MOLECULAR MICROBIOLOGY

Physiological heterogeneity in biofilms

(2008) Philip S. Stewart et al.   NATURE REVIEWS MICROBIOLOGY

agr-Mediated Dispersal of Staphylococcus aureus Biofilms

(2008) Blaise R. Boles et al.   PLoS Pathogens

Why chronic wounds will not heal: a novel hypothesis

(2007) Thomas Bjarnsholt et al.   WOUND REPAIR AND REGENERATION