TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii

A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia

(2019) Tyler Picariello et al.   JOURNAL OF CELL SCIENCE

The dynamin-like protein Fzl promotes thylakoid fusion and resistance to light stress in Chlamydomonas reinhardtii

(2019) Justin Findinier et al.   PLoS Genetics

A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis

(2019) Xiaobo Li et al.   NATURE GENETICS

Efficient Editing of the Nuclear APT Reporter Gene in Chlamydomonas reinhardtii via Expression of a CRISPR-Cas9 Module

(2019) Daniel Guzmán-Zapata et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Proteome of the central apparatus of a ciliary axoneme

(2019) Lei Zhao et al.   JOURNAL OF CELL BIOLOGY

A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism

(2019) Patrice A. Salomé et al.   PLANT CELL

Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus

(2019) Gang Fu et al.   JOURNAL OF CELL BIOLOGY

The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length

(2018) Emily L. Hunter et al.   MOLECULAR BIOLOGY OF THE CELL

Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production

(2018) Ye Sol Shin et al.   BIORESOURCE TECHNOLOGY

In vivo analysis of outer arm dynein transport reveals cargo-specific IFT properties

(2018) Jin Dai et al.   MOLECULAR BIOLOGY OF THE CELL

MAPINS, a highly efficient detection method that identifies insertional mutations and complex DNA rearrangements

(2018) Huawen Lin et al.   PLANT PHYSIOLOGY

CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility

(2018) Noritoshi Shamoto et al.   Cells

Deletion of the chloroplast LTD protein impedes LHCI import and PSI–LHCI assembly in Chlamydomonas reinhardtii

(2017) Jooyeon Jeong et al.   JOURNAL OF EXPERIMENTAL BOTANY

Targeting of Photoreceptor Genes inChlamydomonas reinhardtiivia Zinc-Finger Nucleases and CRISPR/Cas9

(2017) Andre Greiner et al.   PLANT CELL

A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

(2017) Steve V. Pollock et al.   Plant Methods

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin

(2016) Tomohiro Kubo et al.   JOURNAL OF CELL SCIENCE

An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii

(2016) Xiaobo Li et al.   PLANT CELL

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Assembly of IFT Trains at the Ciliary Base Depends on IFT74

(2015) Jason M. Brown et al.   CURRENT BIOLOGY

Tubulin transport by IFT is upregulated during ciliary growth by a cilium-autonomous mechanism

(2015) Julie M. Craft et al.   JOURNAL OF CELL BIOLOGY

Molecular techniques to interrogate and edit theChlamydomonasnuclear genome

(2015) Robert E. Jinkerson et al.   PLANT JOURNAL

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

A Differential Cargo-Loading Model of Ciliary Length Regulation by IFT

(2013) Kathryne N. Wren et al.   CURRENT BIOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus

(2012) J. M. Brown et al.   JOURNAL OF CELL SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

TheChlamydomonas reinhardtiiBBSome is an IFT cargo required for export of specific signaling proteins from flagella

(2009) Karl-Ferdinand Lechtreck et al.   JOURNAL OF CELL BIOLOGY

The UNI1 and UNI2 Genes Function in the Transition of Triplet to Doublet Microtubules between the Centriole and Cilium in Chlamydomonas

(2008) Brian P. Piasecki et al.   MOLECULAR BIOLOGY OF THE CELL