The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

Expression and Characterization of Functional Recombinant Bet v 1.0101 in the Chloroplast of Chlamydomonas reinhardtii

(2017) Sonja Hirschl et al.   INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY

Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii

(2017) Laura Stoffels et al.   PLANT BIOTECHNOLOGY JOURNAL

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

(2016) Thanyanan Wannathong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant Hemagglutinin of Avian Influenza Virus H5 Expressed in the Chloroplast of Chlamydomonas reinhardtii and Evaluation of Its Immunogenicity in Chickens

(2016) Inkar Castellanos-Huerta et al.   AVIAN DISEASES

Development of phytase-expressing chlamydomonas reinhardtii for monogastric animal nutrition

(2016) Fernanda Erpel et al.   BMC BIOTECHNOLOGY

Bioactivity of an antihypertensive peptide expressed in Chlamydomonas reinhardtii

(2016) Celma Estefanía Ochoa-Méndez et al.   JOURNAL OF BIOTECHNOLOGY

Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice

(2016) James A. Gregory et al.   PLANT BIOTECHNOLOGY JOURNAL

Chlamydomonas reinhardtii chloroplasts express an orally immunogenic protein targeting the p210 epitope implicated in atherosclerosis immunotherapies

(2016) Josué I. Beltrán-López et al.   PLANT CELL REPORTS

Transformation of the Cyanidioschyzon merolae chloroplast genome: prospects for understanding chloroplast function in extreme environments

(2016) Maksymilian Zienkiewicz et al.   PLANT MOLECULAR BIOLOGY

Efficient targeting of recombinant proteins to the thylakoid lumen in Chlamydomonas reinhardtii using a bacterial Tat signal peptide

(2016) Julie A.Z. Zedler et al.   Algal Research-Biomass Biofuels and Bioproducts

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Engineering Plastid Genomes: Methods, Tools, and Applications in Basic Research and Biotechnology

(2015) Ralph Bock   Annual Review of Plant Biology

Automated nutrient screening system enables high-throughput optimisation of microalgae production conditions

(2015) Khairul Adzfa Radzun et al.   Biotechnology for Biofuels

Advanced technologies for improved expression of recombinant proteins in bacteria: perspectives and applications

(2015) Sanjeev K. Gupta et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

A rapid, modular and marker-free chloroplast expression system for the green alga Chlamydomonas reinhardtii

(2015) Ivo Bertalan et al.   JOURNAL OF BIOTECHNOLOGY

Expression and membrane-targeting of an active plant cytochrome P450 in the chloroplast of the green alga Chlamydomonas reinhardtii

(2015) Doris Gangl et al.   PHYTOCHEMISTRY

Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast ofChlamydomonas reinhardtii

(2015) Rosanna E. B. Young et al.   PLANT BIOTECHNOLOGY JOURNAL

EstablishingChlamydomonas reinhardtiias an industrial biotechnology host

(2015) Mark A. Scaife et al.   PLANT JOURNAL

A mini review: photobioreactors for large scale algal cultivation

(2015) Prabuddha L. Gupta et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

(2015) Javier A. Gimpel et al.   ACS Synthetic Biology

Production of recombinant proteins in microalgae at pilot greenhouse scale

(2014) Javier A. Gimpel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Domestication of the green alga Chlorella sorokiniana: reduction of antenna size improves light-use efficiency in a photobioreactor

(2014) Stefano Cazzaniga et al.   Biotechnology for Biofuels

Phytate in pig and poultry nutrition

(2014) E. Humer et al.   JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION

Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum

(2014) Wei-Hong Xie et al.   MARINE BIOTECHNOLOGY

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Algal omics: unlocking bioproduct diversity in algae cell factories

(2014) Michael T. Guarnieri et al.   PHOTOSYNTHESIS RESEARCH

Algal chloroplast produced camelid VH H antitoxins are capable of neutralizing botulinum neurotoxin

(2014) Daniel J. Barrera et al.   PLANT BIOTECHNOLOGY JOURNAL

Gateway-Assisted Vector Construction to Facilitate Expression of Foreign Proteins in the Chloroplast of Single Celled Algae

(2014) Melanie Oey et al.   PLoS One

Molecular Approach to Allergy Diagnosis and Therapy

(2014) Fatima Ferreira et al.   YONSEI MEDICAL JOURNAL

Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

(2014) Alma Lorena Almaraz-Delgado et al.   AMB Express

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

(2013) James A. Gregory et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of anti-cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners

(2013) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae

(2013) René H Wijffels et al.   CURRENT OPINION IN BIOTECHNOLOGY

Advances in microalgae engineering and synthetic biology applications for biofuel production

(2013) Javier A Gimpel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Synthetic biology in plastids

(2013) Lars B. Scharff et al.   PLANT JOURNAL

A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection

(2013) Olivia C. Demurtas et al.   PLoS One

Genetic engineering of algal chloroplasts: Progress and prospects

(2013) S. Purton et al.   RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii

(2012) Carla S. Jones et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Method to assemble and integrate biochemical pathways into the chloroplast genome ofChlamydomonas reinhardtii

(2012) Samaneh Noor-Mohammadi et al.   BIOTECHNOLOGY AND BIOENGINEERING

CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES)1

(2012) Carla L. Gutiérrez et al.   JOURNAL OF PHYCOLOGY

HOW MANY SPECIES OF ALGAE ARE THERE?

(2012) Michael D. Guiry   JOURNAL OF PHYCOLOGY

Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii

(2012) Nohemí Campos-Quevedo et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

(2012) James A. Gregory et al.   PLoS One

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

(2012) M. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic Oligonucleotide Libraries Reveal Novel Regulatory Elements in Chlamydomonas Chloroplast mRNAs

(2012) Elizabeth A. Specht et al.   ACS Synthetic Biology

Production of recombinant enzymes in the marine alga Dunaliella tertiolecta

(2012) D. Ryan Georgianna et al.   Algal Research-Biomass Biofuels and Bioproducts

Steps toward a globally available malaria vaccine

(2012) Carla S. Jones et al.   Bioengineered

Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks

(2011) So-Mi Yoon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Best practices in heterotrophic high-cell-density microalgal processes: achievements, potential and possible limitations

(2011) Fabian Bumbak et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

An exogenous chloroplast genome for complex sequence manipulation in algae

(2011) Bryan M. O'Neill et al.   NUCLEIC ACIDS RESEARCH

The microbial cell factory

(2011) Cormac D. Murphy   ORGANIC & BIOMOLECULAR CHEMISTRY

Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5′ untranslated region optimization

(2011) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Chloroplast genomes of photosynthetic eukaryotes

(2011) Beverley R. Green   PLANT JOURNAL

Expression of Plastid Genes: Organelle-Specific Elaborations on a Prokaryotic Scaffold

(2011) A. Barkan   PLANT PHYSIOLOGY

Molecular Factors Affecting the Accumulation of Recombinant Proteins in the Chlamydomonas reinhardtii Chloroplast

(2010) Anna T. Coragliotti et al.   MOLECULAR BIOTECHNOLOGY

Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

(2010) Laure Michelet et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

(2010) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Synthesis and assembly of a full-length human monoclonal antibody in algal chloroplasts

(2009) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

(2009) Imke A.J. Dreesen et al.   JOURNAL OF BIOTECHNOLOGY

A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)

(2008) Xiaofeng Wang et al.   BMC BIOTECHNOLOGY