Mechanisms of substrate recognition and N6-methyladenosine demethylation revealed by crystal structures of ALKBH5–RNA complexes

Rational Design of Novel Anticancer Small-Molecule RNA m6A Demethylase ALKBH5 Inhibitors

(2021) Simona Selberg et al.   ACS Omega

N6-methyladenosine binding induces a metal-centered rearrangement that activates the human RNA demethylase Alkbh5

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RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia

(2020) Chao Shen et al.   Cell Stem Cell

RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner

(2020) Xingya Guo et al.   Molecular Cancer

The potential role of RNA N6-methyladenosine in Cancer progression

(2020) Tianyi Wang et al.   Molecular Cancer

ALKBH5 suppresses malignancy of hepatocellular carcinoma via m6A-guided epigenetic inhibition of LYPD1

(2020) Yunhao Chen et al.   Molecular Cancer

Distinct RNAN-demethylation pathways catalyzed by nonheme iron ALKBH5 and FTO enzymes enable regulation of formaldehyde release rates

(2020) Joel D. W. Toh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ALKBH5 inhibited cell proliferation and sensitized bladder cancer cells to cisplatin by m6A-CK2α mediated glycolysis

(2020) Hao Yu et al.   Molecular Therapy-Nucleic Acids

Structural insights into FTO’s catalytic mechanism for the demethylation of multiple RNA substrates

(2019) Xiao Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

FTO controls reversible m6Am RNA methylation during snRNA biogenesis

(2019) Jan Mauer et al.   Nature Chemical Biology

Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix

(2019) Dorothee Liebschner et al.   Acta Crystallographica Section D-Structural Biology

Direct RNA sequencing enables m6A detection in endogenous transcript isoforms at base-specific resolution

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Association of N6-methyladenosine with viruses and related diseases

(2019) Fang Wu et al.   Virology Journal

2-Oxoglutarate-Dependent Oxygenases

(2018) Md. Saiful Islam et al.   Annual Review of Biochemistry

Dynamic transcriptomic m6A decoration: writers, erasers, readers and functions in RNA metabolism

(2018) Ying Yang et al.   CELL RESEARCH

NMR analyses on N-hydroxymethylated nucleobases – implications for formaldehyde toxicity and nucleic acid demethylases

(2018) S. Shishodia et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Crystal structure of the RNA demethylase ALKBH5 from zebrafish

(2014) Weizhong Chen et al.   FEBS LETTERS

Structures of Human ALKBH5 Demethylase Reveal a Unique Binding Mode for Specific Single-strandedN6-Methyladenosine RNA Demethylation

(2014) Chao Xu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structures of the Human RNA Demethylase Alkbh5 Reveal Basis for Substrate Recognition

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Alignment-Annotator web server: rendering and annotating sequence alignments

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Deciphering key features in protein structures with the new ENDscript server

(2014) Xavier Robert et al.   NUCLEIC ACIDS RESEARCH

Structure of human RNA N6-methyladenine demethylase ALKBH5 provides insights into its mechanisms of nucleic acid recognition and demethylation

(2014) WeiShen Aik et al.   NUCLEIC ACIDS RESEARCH

Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation

(2014) John S. Scotti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure of TET2-DNA Complex: Insight into TET-Mediated 5mC Oxidation

(2013) Lulu Hu et al.   CELL

Structural Basis for Inhibition of the Fat Mass and Obesity Associated Protein (FTO)

(2013) WeiShen Aik et al.   JOURNAL OF MEDICINAL CHEMISTRY

Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA

(2013) Hideharu Hashimoto et al.   NATURE

FTO-mediated formation of N6-hydroxymethyladenosine and N6-formyladenosine in mammalian RNA

(2013) Ye Fu et al.   Nature Communications

Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons

(2012) Kate D. Meyer et al.   CELL

ALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility

(2012) Guanqun Zheng et al.   MOLECULAR CELL

Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq

(2012) Dan Dominissini et al.   NATURE

Duplex interrogation by a direct DNA repair protein in search of base damage

(2012) Chengqi Yi et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Transfer-PCR (TPCR): A highway for DNA cloning and protein engineering

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N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO

(2011) Guifang Jia et al.   Nature Chemical Biology

Human AlkB Homologue 5 Is a Nuclear 2-Oxoglutarate Dependent Oxygenase and a Direct Target of Hypoxia-Inducible Factor 1α (HIF-1α)

(2011) Armin Thalhammer et al.   PLoS One

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Structural and Mechanistic Studies on γ-Butyrobetaine Hydroxylase

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High-throughput production of human proteins for crystallization: The SGC experience

(2010) Pavel Savitsky et al.   JOURNAL OF STRUCTURAL BIOLOGY

Crystal structure of the FTO protein reveals basis for its substrate specificity

(2010) Zhifu Han et al.   NATURE

Structural and Mutational Analysis of Escherichia coli AlkB Provides Insight into Substrate Specificity and DNA Damage Searching

(2010) Paul J. Holland et al.   PLoS One

xia2: an expert system for macromolecular crystallography data reduction

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Structural Basis for Binding of Hypoxia-Inducible Factor to the Oxygen-Sensing Prolyl Hydroxylases

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Crystal structures of DNA/RNA repair enzymes AlkB and ABH2 bound to dsDNA

(2008) Cai-Guang Yang et al.   NATURE