Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization

Mobility of kinetochore proteins measured by FRAP analysis in living cells

(2022) Reito Watanabe et al.   CHROMOSOME RESEARCH

Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome

(2022) Stanislau Yatskevich et al.   SCIENCE

Cryo‐EM structure of the CENP‐A nucleosome in complex with phosphorylated CENP‐C

(2021) Mariko Ariyoshi et al.   EMBO JOURNAL

A high-resolution protein architecture of the budding yeast genome

(2021) Matthew J. Rossi et al.   NATURE

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Assembly principles and stoichiometry of a complete human kinetochore module

(2021) Kai Walstein et al.   Science Advances

DeepEMhancer: a deep learning solution for cryo-EM volume post-processing

(2021) Ruben Sanchez-Garcia et al.   Communications Biology

Diverse mechanisms of centromere specification

(2021) Barbara G. Mellone et al.   CURRENT BIOLOGY

Structural basis for centromere maintenance by Drosophila CENP ‐A chaperone CAL 1

(2020) Bethan Medina‐Pritchard et al.   EMBO JOURNAL

What makes a centromere?

(2020) Paul B. Talbert et al.   EXPERIMENTAL CELL RESEARCH

The Structural Basis for Kinetochore Stabilization by Cnn1/CENP-T

(2020) Stephen M. Hinshaw et al.   CURRENT BIOLOGY

The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle

(2020) Kathryn Kixmoeller et al.   Open Biology

A genetic memory initiates the epigenetic loop necessary to preserve centromere position

(2020) Sebastian Hoffmann et al.   EMBO JOURNAL

Crystal structure of the Cenp-HIKHead-TW sub-module of the inner kinetochore CCAN complex

(2020) Ziguo Zhang et al.   NUCLEIC ACIDS RESEARCH

UCSF ChimeraX : Structure visualization for researchers, educators, and developers

(2020) Eric F. Pettersen et al.   PROTEIN SCIENCE

The nucleosomes that mark centromere location on chromosomes old and new

(2019) Craig W. Gambogi et al.   Essays in Biochemistry

Mosaic origin of the eukaryotic kinetochore

(2019) Eelco C. Tromer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The structure of the Ctf19c/CCAN from budding yeast

(2019) Stephen M Hinshaw et al.   eLife

Structure of the Human Core Centromeric Nucleosome Complex

(2019) Praveen Kumar Allu et al.   CURRENT BIOLOGY

Structures of CENP-C cupin domains at regional centromeres reveal unique patterns of dimerization and recruitment functions for the inner pocket

(2019) Jennifer K. Chik et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Namdinator – automatic molecular dynamics flexible fitting of structural models into cryo-EM and crystallography experimental maps

(2019) Rune Thomas Kidmose et al.   IUCrJ

CENP ‐C unwraps the human CENP ‐A nucleosome through the H2A C‐terminal tail

(2019) Ahmad Ali‐Ahmad et al.   EMBO REPORTS

Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres

(2019) María Isabel Navarro-Mendoza et al.   CURRENT BIOLOGY

Seeing is believing: our evolving view of kinetochore structure, composition, and assembly

(2019) Grace Hamilton et al.   CURRENT OPINION IN CELL BIOLOGY

Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome

(2019) Kaige Yan et al.   NATURE

Molecular basis for CENP-N recognition of CENP-A nucleosome on the human kinetochore

(2018) Tian Tian et al.   CELL RESEARCH

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

(2018) Takanori Nakane et al.   eLife

Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80

(2018) Marion E. Pesenti et al.   MOLECULAR CELL

Molecular basis of CENP-C association with the CENP-A nucleosome at yeast centromeres

(2017) Hua Xiao et al.   GENES & DEVELOPMENT

Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points

(2017) Yael Nechemia-Arbely et al.   JOURNAL OF CELL BIOLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N

(2017) Sagar Chittori et al.   SCIENCE

Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition

(2017) Lucie Y. Guo et al.   Nature Communications

Decoding the centromeric nucleosome through CENP-N

(2017) Satyakrishna Pentakota et al.   eLife

CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading

(2017) Dongqing Pan et al.   eLife

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

(2017) Toshio Moriya et al.   Jove-Journal of Visualized Experiments

A pipeline approach to single-particle processing inRELION

(2017) Rafael Fernandez-Leiro et al.   Acta Crystallographica Section D-Structural Biology

Insights from biochemical reconstitution into the architecture of human kinetochores

(2016) John R. Weir et al.   NATURE

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function

(2015) Daniele Fachinetti et al.   DEVELOPMENTAL CELL

Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of CENP-N

(2015) Junnan Fang et al.   GENES & DEVELOPMENT

CENP-C is a blueprint for constitutive centromere–associated network assembly within human kinetochores

(2015) Kerstin Klare et al.   JOURNAL OF CELL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Dynamic changes in CCAN organization through CENP-C during cell-cycle progression

(2015) Harsh Nagpal et al.   MOLECULAR BIOLOGY OF THE CELL

The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface

(2015) Kara L. McKinley et al.   MOLECULAR CELL

The molecular basis for centromere identity and function

(2015) Kara L. McKinley et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

DNA Topology and Global Architecture of Point Centromeres

(2015) Ofelia Díaz-Ingelmo et al.   Cell Reports

A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A

(2014) Peter Hornung et al.   JOURNAL OF CELL BIOLOGY

The pseudo GTPase CENP-M drives human kinetochore assembly

(2014) Federica Basilico et al.   eLife

The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo

(2014) Steven Henikoff et al.   eLife

Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres

(2014) Jan Wisniewski et al.   eLife

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Solo or doppio: how many CENP-As make a centromeric nucleosome?

(2013) Elaine M Dunleavy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The octamer is the major form of CENP-A nucleosomes at human centromeres

(2013) Dan Hasson et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The centromeric nucleosome-like CENP–T–W–S–X complex induces positive supercoils into DNA

(2013) Kozo Takeuchi et al.   NUCLEIC ACIDS RESEARCH

Reconstitution of hemisomes on budding yeast centromeric DNA

(2013) Takehito Furuyama et al.   NUCLEIC ACIDS RESEARCH

A Conserved Mechanism for Centromeric Nucleosome Recognition by Centromere Protein CENP-C

(2013) H. Kato et al.   SCIENCE

An Iml3-Chl4 Heterodimer Links the Core Centromere to Factors Required for Accurate Chromosome Segregation

(2013) Stephen M. Hinshaw et al.   Cell Reports

Comparison of Segger and other methods for segmentation and rigid-body docking of molecular components in Cryo-EM density maps

(2012) Grigore Pintilie et al.   BIOPOLYMERS

CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold

(2012) Tatsuya Nishino et al.   CELL

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

A cell-free system for functional centromere and kinetochore assembly

(2012) Annika Guse et al.   Nature Protocols

Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex

(2012) C. Brocker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Epigenetic Centromere Propagation and the Nature of CENP-A Nucleosomes

(2011) Ben E. Black et al.   CELL

Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes

(2011) Karen E. Gascoigne et al.   CELL

Direct Binding of Cenp-C to the Mis12 Complex Joins the Inner and Outer Kinetochore

(2011) Emanuela Screpanti et al.   CURRENT BIOLOGY

UCSF Chimera, MODELLER, and IMP: An integrated modeling system

(2011) Zheng Yang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Nonhistone Scm3 Binds to AT-Rich DNA to Organize Atypical Centromeric Nucleosome of Budding Yeast

(2011) Hua Xiao et al.   MOLECULAR CELL

Crystal structure of the human centromeric nucleosome containing CENP-A

(2011) Hiroaki Tachiwana et al.   NATURE

Tripartite organization of centromeric chromatin in budding yeast

(2011) K. Krassovsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere

(2011) H. A. Cole et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recognition of the centromere-specific histone Cse4 by the chaperone Scm3

(2011) U.-S. Cho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Histone H3-variant Cse4-induced positive DNA supercoiling in the yeast plasmid has implications for a plasmid origin of a chromosome centromere

(2011) C.-C. Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and Scm3-mediated assembly of budding yeast centromeric nucleosomes

(2011) Mekonnen Lemma Dechassa et al.   Nature Communications

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Dual recognition of CENP-A nucleosomes is required for centromere assembly

(2010) Christopher W. Carroll et al.   JOURNAL OF CELL BIOLOGY

The docking domain of histone H2A is required for H1 binding and RSC-mediated nucleosome remodeling

(2010) Manu Shubhdarshan Shukla et al.   NUCLEIC ACIDS RESEARCH

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Centromeric Nucleosomes Induce Positive DNA Supercoils

(2009) Takehito Furuyama et al.   CELL

Fission Yeast Scm3 Mediates Stable Assembly of Cnp1/CENP-A into Centromeric Chromatin

(2009) Jessica S. Williams et al.   MOLECULAR CELL

Cse4 Is Part of an Octameric Nucleosome in Budding Yeast

(2009) Raymond Camahort et al.   MOLECULAR CELL

Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N

(2009) Christopher W. Carroll et al.   NATURE CELL BIOLOGY

CCAN Makes Multiple Contacts with Centromeric DNA to Provide Distinct Pathways to the Outer Kinetochore

(2008) Tetsuya Hori et al.   CELL

Structural and Functional Dissection of Mif2p, a Conserved DNA-binding Kinetochore Protein

(2008) R. L. Cohen et al.   MOLECULAR BIOLOGY OF THE CELL