Camelid Single-Domain Antibodies: Promises and Challenges as Lifesaving Treatments

Easily Established and Multifunctional Synthetic Nanobody Libraries as Research Tools

(2022) Bingying Liu et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Research Progress and Applications of Multivalent, Multispecific and Modified Nanobodies for Disease Treatment

(2022) Jiewen Wang et al.   Frontiers in Immunology

Ozoralizumab, a Humanized Anti-TNFα NANOBODY® Compound, Exhibits Efficacy Not Only at the Onset of Arthritis in a Human TNF Transgenic Mouse but Also During Secondary Failure of Administration of an Anti-TNFα IgG

(2022) Chihiro Ishiwatari-Ogata et al.   Frontiers in Immunology

A broad-spectrum nanobody targeting the C-terminus of the hepatitis B surface antigen for chronic hepatitis B infection therapy

(2022) Yue Wang et al.   ANTIVIRAL RESEARCH

Biparatopic sybodies neutralize SARS‐CoV‐2 variants of concern and mitigate drug resistance

(2022) Justin D Walter et al.   EMBO REPORTS

FDA approves second BCMA-targeted CAR-T cell therapy

(2022) Asher Mullard   NATURE REVIEWS DRUG DISCOVERY

Antibodies to watch in 2021

(2021) Hélène Kaplon et al.   mAbs

Immunogenicity and humanization of single‐domain antibodies

(2021) Martin A. Rossotti et al.   FEBS Journal

Immunogenicity Risk Profile of Nanobodies

(2021) Chloé Ackaert et al.   Frontiers in Immunology

Incorporation of a Novel CD16-Specific Single-Domain Antibody into Multispecific Natural Killer Cell Engagers With Potent ADCC

(2021) Henk van Faassen et al.   MOLECULAR PHARMACEUTICS

FDA approves 100th monoclonal antibody product

(2021) Asher Mullard   NATURE REVIEWS DRUG DISCOVERY

Shark New Antigen Receptor (IgNAR): Structure, Characteristics and Potential Biomedical Applications

(2021) Salma Nassor Juma et al.   Cells

Neutralization of SARS‐CoV‐2 by highly potent, hyperthermostable, and mutation‐tolerant nanobodies

(2021) Thomas Güttler et al.   EMBO JOURNAL

Brain Disposition of Antibody-Based Therapeutics: Dogma, Approaches and Perspectives

(2021) Aida Kouhi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants

(2021) Jianliang Xu et al.   NATURE

Molecularly Engineered Nanobodies for Tunable Pharmacokinetics and Drug Delivery

(2020) Patrick M. Glassman et al.   BIOCONJUGATE CHEMISTRY

Serum albumin‐binding V H Hs with variable pH sensitivities enable tailored half‐life extension of biologics

(2020) Henk van Faassen et al.   FASEB JOURNAL

A Novel Anti-Kv10.1 Nanobody Fused to Single-Chain TRAIL Enhances Apoptosis Induction in Cancer Cells

(2020) Franziska Hartung et al.   Frontiers in Pharmacology

Aducanumab, gantenerumab, BAN2401, and ALZ-801—the first wave of amyloid-targeting drugs for Alzheimer’s disease with potential for near term approval

(2020) Martin Tolar et al.   Alzheimers Research & Therapy

Nanobodies: Next Generation of Cancer Diagnostics and Therapeutics

(2020) Emily Y. Yang et al.   Frontiers in Oncology

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction

(2020) Leo Hanke et al.   Nature Communications

Solubility, Stability, and Avidity of Recombinant Antibody Fragments Expressed in Microorganisms

(2020) Tae Hyun Kang et al.   Frontiers in Microbiology

Brain Delivery of Single-Domain Antibodies: A Focus on VHH and VNAR

(2020) Elodie Pothin et al.   Pharmaceutics

Cost effectiveness of caplacizumab in acquired thrombotic thrombocytopenic purpura

(2020) George Goshua et al.   BLOOD

Applications of Nanobodies

(2020) Serge Muyldermans   Annual Review of Animal Biosciences

An Inside Job: Applications of Intracellular Single Domain Antibodies

(2020) Eline Soetens et al.   Biomolecules

Llama peripheral B-cell populations producing conventional and heavy chain-only IgG subtypes are phenotypically indistinguishable but immunogenetically distinct

(2019) Kevin A. Henry et al.   IMMUNOGENETICS

Clinical pharmacology of caplacizumab for the treatment of patients with acquired thrombotic thrombocytopenic purpura

(2019) Maria Laura Sargentini-Maier et al.   Expert Review of Clinical Pharmacology

Nanobody approval gives domain antibodies a boost

(2019) Chris Morrison   NATURE REVIEWS DRUG DISCOVERY

Nanobodies that Neutralize HIV

(2019) Weiss et al.   Vaccines

Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies

(2019) Yining Zhao et al.   CANCER BIOLOGY & THERAPY

A Novel Single Domain Antibody Targeting FliC Flagellin of Salmonella enterica for Effective Inhibition of Host Cell Invasion

(2019) Jennifer Huen et al.   Frontiers in Microbiology

The Camel Adaptive Immune Receptors Repertoire as a Singular Example of Structural and Functional Genomics

(2019) Salvatrice Ciccarese et al.   Frontiers in Genetics

Size and affinity kinetics of nanobodies influence targeting and penetration of solid tumours

(2019) Pieterjan Debie et al.   JOURNAL OF CONTROLLED RELEASE

Delivery of ALX-0171 by inhalation greatly reduces respiratory syncytial virus disease in newborn lambs

(2018) Alejandro Larios Mora et al.   mAbs

Molecular Imaging of Mesothelin-Expressing Ovarian Cancer with a Human and Mouse Cross-Reactive Nanobody

(2018) Andrew M. Prantner et al.   MOLECULAR PHARMACEUTICS

Structural Basis of Epitope Recognition by Heavy-Chain Camelid Antibodies

(2018) Uroš Zavrtanik et al.   JOURNAL OF MOLECULAR BIOLOGY

 Single-domain antibody fragments derived from heavy-chain antibodies: a review

(2018) L. Eyer et al.   VETERINARNI MEDICINA

Universal protection against influenza infection by a multidomain antibody to influenza hemagglutinin

(2018) Nick S. Laursen et al.   SCIENCE

Understanding the Significance and Implications of Antibody Numbering and Antigen-Binding Surface/Residue Definition

(2018) Mathieu Dondelinger et al.   Frontiers in Immunology

An efficient constitutive expression system for Anti-CEACAM5 nanobody production in the yeast Pichia pastoris

(2018) Quan Chen et al.   PROTEIN EXPRESSION AND PURIFICATION

Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state

(2017) Iebe Rossey et al.   Nature Communications

Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence

(2017) Yariv Mazor et al.   Scientific Reports

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein–Protein Interaction Analysis, and Protein Function Exploration

(2017) Els Beghein et al.   Frontiers in Immunology

Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook

(2017) Mehdi Arbabi-Ghahroudi   Frontiers in Immunology

VHH antibodies: emerging reagents for the analysis of environmental chemicals

(2016) Candace S. Bever et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Single domain antibody-based bispecific antibody induces potent specific anti-tumor activity

(2016) Jing Li et al.   CANCER BIOLOGY & THERAPY

A novel bispecific antibody, BiSS, with potent anti-cancer activities

(2016) Bin Dong et al.   CANCER BIOLOGY & THERAPY

Nanobodies as therapeutics: big opportunities for small antibodies

(2016) Sophie Steeland et al.   DRUG DISCOVERY TODAY

Brain penetration, target engagement, and disposition of the blood–brain barrier-crossing bispecific antibody antagonist of metabotropic glutamate receptor type 1

(2016) Carl I. Webster et al.   FASEB JOURNAL

Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications

(2016) Yongzhong Wang et al.   International Journal of Nanomedicine

Development by Genetic Immunization of Monovalent Antibodies (Nanobodies) Behaving as Antagonists of the Human ChemR23 Receptor

(2016) Xavier Peyrassol et al.   JOURNAL OF IMMUNOLOGY

Caplacizumab for Acquired Thrombotic Thrombocytopenic Purpura

(2016) Flora Peyvandi et al.   NEW ENGLAND JOURNAL OF MEDICINE

NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies

(2016) Sandrine Moutel et al.   eLife

A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies

(2016) Kevin A. Henry et al.   PLoS One

Enzyme-Mediated Modification of Single-Domain Antibodies for Imaging Modalities with Different Characteristics

(2015) Mohammad Rashidian et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Blinatumomab: First Global Approval

(2015) Mark Sanford   DRUGS

Nanobodies as Probes for Protein Dynamicsin Vitroand in Cells

(2015) Oleg Y. Dmitriev et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nanobodies and recombinant binders in cell biology

(2015) Jonas Helma et al.   JOURNAL OF CELL BIOLOGY

Phase I Study of 68Ga-HER2-Nanobody for PET/CT Assessment of HER2 Expression in Breast Carcinoma

(2015) M. Keyaerts et al.   JOURNAL OF NUCLEAR MEDICINE

Camelid Ig V genes reveal significant human homology not seen in therapeutic target genes, providing for a powerful therapeutic antibody platform

(2015) Alex Klarenbeek et al.   mAbs

Identification of cross-reactive single-domain antibodies against serum albumin using next-generation DNA sequencing

(2015) Kevin A. Henry et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The preclinical pharmacology of the high affinity anti-IL-6R Nanobody® ALX-0061 supports its clinical development in rheumatoid arthritis

(2015) Maarten Van Roy et al.   ARTHRITIS RESEARCH & THERAPY

Characterization and applications of Nanobodies against human procalcitonin selected from a novel naïve Nanobody phage display library

(2015) Junrong Yan et al.   JOURNAL OF NANOBIOTECHNOLOGY

A novel platform for engineering blood-brain barrier-crossing bispecific biologics

(2014) Graham K. Farrington et al.   FASEB JOURNAL

Tetrameric and Homodimeric Camelid IgGs Originate from the Same IgH Locus

(2014) I. Achour et al.   JOURNAL OF IMMUNOLOGY

Restricted Diversity of Antigen Binding Residues of Antibodies Revealed by Computational Alanine Scanning of 227 Antibody–Antigen Complexes

(2014) Gautier Robin et al.   JOURNAL OF MOLECULAR BIOLOGY

Potential candidate camelid antibodies for the treatment of protein-misfolding diseases

(2014) Monique Antoinette David et al.   JOURNAL OF NEUROIMMUNOLOGY

Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications

(2014) Junrong Yan et al.   Journal of Translational Medicine

Structural insights and biomedical potential of IgNAR scaffolds from sharks

(2014) Stefan Zielonka et al.   mAbs

The therapeutic monoclonal antibody market

(2014) Dawn M Ecker et al.   mAbs

Pentavalent Single-Domain Antibodies Reduce Campylobacter jejuni Motility and Colonization in Chickens

(2014) Ali Riazi et al.   PLoS One

Protease-resistant single-domain antibodies inhibit Campylobacter jejuni motility

(2014) G. Hussack et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Nanobody: The “Magic Bullet” for Molecular Imaging?

(2014) Rubel Chakravarty et al.   Theranostics

Molecular Evolution of Broadly Neutralizing Llama Antibodies to the CD4-Binding Site of HIV-1

(2014) Laura E. McCoy et al.   PLoS Pathogens

IgG Subclasses and Allotypes: From Structure to Effector Functions

(2014) Gestur Vidarsson et al.   Frontiers in Immunology

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

(2013) Volker Jäger et al.   BMC BIOTECHNOLOGY

Llama-derived Single Variable Domains (Nanobodies) Directed against Chemokine Receptor CXCR7 Reduce Head and Neck Cancer Cell Growthin Vivo

(2013) David Maussang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single-Domain Antibody-Based and Linker-Free Bispecific Antibodies Targeting Fc RIII Induce Potent Antitumor Activity without Recruiting Regulatory T Cells

(2013) C. Rozan et al.   MOLECULAR CANCER THERAPEUTICS

Cell-penetrating anti-GFAP VHH and corresponding fluorescent fusion protein VHH-GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging

(2012) Tengfei Li et al.   FASEB JOURNAL

Pre-clinical studies of toxin-specific Nanobodies: Evidence of in vivo efficacy to prevent fatal disturbances provoked by scorpion envenoming

(2012) Issam Hmila et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Using microdialysis to analyse the passage of monovalent nanobodies through the blood-brain barrier

(2011) G Caljon et al.   BRITISH JOURNAL OF PHARMACOLOGY

Dual Beneficial Effect of Interloop Disulfide Bond for Single Domain Antibody Fragments

(2011) Jochen Govaert et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Boosting Brain Uptake of a Therapeutic Antibody by Reducing Its Affinity for a Transcytosis Target

(2011) Y. J. Yu et al.   Science Translational Medicine

Structural analysis of effector functions related motifs, complement activation and hemagglutinating activities in Lama glama heavy chain antibodies

(2011) Natalia Saccodossi et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

A Case Of Convergence: Why Did a Simple Alternative to Canonical Antibodies Arise in Sharks and Camels?

(2011) Martin F. Flajnik et al.   PLOS BIOLOGY

Research and development of next generation of antibody-based therapeutics

(2010) Jing Li et al.   ACTA PHARMACOLOGICA SINICA

A Novel Promiscuous Class of Camelid Single-Domain Antibody Contributes to the Antigen-Binding Repertoire

(2010) N. Deschacht et al.   JOURNAL OF IMMUNOLOGY

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

(2009) Janusz Wesolowski et al.   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

Development trends for therapeutic antibody fragments

(2009) Aaron L Nelson et al.   NATURE BIOTECHNOLOGY

Modulation of protein properties in living cells using nanobodies

(2009) Axel Kirchhofer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Isolation and Functional Characterization of Single Domain Antibody Modulators of Caspase-3 and Apoptosis

(2008) Katrina McGonigal et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Single-domain antibodies as building blocks for novel therapeutics

(2008) D SAERENS et al.   CURRENT OPINION IN PHARMACOLOGY

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cloning, expression, and characterization of a single-domain antibody fragment with affinity for 15-acetyl-deoxynivalenol

(2008) Patrick J. Doyle et al.   MOLECULAR IMMUNOLOGY

Single-domain antibody fragments with high conformational stability

(2002) Mireille Dumoulin et al.   PROTEIN SCIENCE