Developing all-in-one virus-like particles for Cas9 mRNA/single guide RNA co-delivery and aptamer-containing lentiviral vectors for improved gene expression

CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles

(2022) Joffrey Mianné et al.   BMC BIOLOGY

Improved alpharetrovirus-based Gag.MS2 particles for efficient and transient delivery of CRISPR-Cas9 into target cells

(2022) Yvonne Baron et al.   Molecular Therapy-Nucleic Acids

Targeting herpes simplex virus with CRISPR–Cas9 cures herpetic stromal keratitis in mice

(2021) Di Yin et al.   NATURE BIOTECHNOLOGY

Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice

(2021) Sikai Ling et al.   Nature Biomedical Engineering

Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like Particles Show High On-Target Base Editing and Undetectable RNA Off-Target Activities

(2021) Pin Lyu et al.   CRISPR Journal

Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering

(2021) Jennifer R. Hamilton et al.   Cell Reports

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

(2020) Peter Gee et al.   Nature Communications

Highly efficient ‘hit-and-run’ genome editing with unconcentrated lentivectors carrying Vpr.Prot.Cas9 protein produced from RRE-containing transcripts

(2020) Ivana Indikova et al.   NUCLEIC ACIDS RESEARCH

Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency

(2020) Eirini Vamva et al.   Molecular Therapy-Methods & Clinical Development

Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing

(2019) Baisong Lu et al.   NUCLEIC ACIDS RESEARCH

CRISPResso2 provides accurate and rapid genome editing sequence analysis

(2019) Kendell Clement et al.   NATURE BIOTECHNOLOGY

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing

(2019) Pin Lyu et al.   NUCLEIC ACIDS RESEARCH

TraFo-CRISPR: Enhanced Genome Engineering by Transient Foamy Virus Vector-Mediated Delivery of CRISPR/Cas9 Components

(2019) Fabian Lindel et al.   Molecular Therapy-Nucleic Acids

Robust Enhancement of Lentivirus Production by Promoter Activation

(2018) Naoto Suzuki et al.   Scientific Reports

Transient retrovirus-based CRISPR/Cas9 all-in-one particles for efficient, targeted gene knockout

(2018) Yvonne Knopp et al.   Molecular Therapy-Nucleic Acids

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

(2018) Philippe E. Mangeot et al.   Nature Communications

Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair

(2018) Eirini M. Kallimasioti-Pazi et al.   PLOS BIOLOGY

Development of the First World Health Organization Lentiviral Vector Standard: Toward the Production Control and Standardization of Lentivirus-Based Gene Therapy Products

(2017) Yuan Zhao et al.   Human Gene Therapy Methods

No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells

(2017) Parisa Javidi-Parsijani et al.   PLoS One

Cas-analyzer: an online tool for assessing genome editing results using NGS data

(2016) Jeongbin Park et al.   BIOINFORMATICS

Lentivirus pre-packed with Cas9 protein for safer gene editing

(2016) J G Choi et al.   GENE THERAPY

Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow

(2016) Hanhui Ma et al.   NATURE BIOTECHNOLOGY

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

(2016) Hao Yin et al.   NATURE BIOTECHNOLOGY

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

(2016) R Stefan Isaac et al.   eLife

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

(2016) Max A Horlbeck et al.   eLife

Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro

(2015) John M. Hinz et al.   BIOCHEMISTRY

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles

(2015) Anne Prel et al.   Molecular Therapy-Methods & Clinical Development

Enhanced lentiviral vector production in 293FT cells expressing Siglec-9

(2014) Toru Shoji et al.   CYTOTECHNOLOGY

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

(2013) Thomas J. Cradick et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Tracking genome engineering outcome at individual DNA breakpoints

(2011) Michael T Certo et al.   NATURE METHODS

Creating Higher Titer Lentivirus with Caffeine

(2010) Brian L. Ellis et al.   HUMAN GENE THERAPY

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

(2009) Sven Ansorge et al.   JOURNAL OF GENE MEDICINE

Increased virus replication in mammalian cells by blocking intracellular innate defense responses

(2008) W de Vries et al.   GENE THERAPY