Technology insight: Plant-derived vesicles—How far from the clinical biotherapeutics and therapeutic drug carriers?

Low concentration of polyethylene glycol facilitates separation of extracellular vesicles from bronchoalveolar lavage fluid

(2021) Heedoo Lee et al.   AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY

A Biomimetic Drug Delivery System by Integrating Grapefruit Extracellular Vesicles and Doxorubicin-Loaded Heparin-Based Nanoparticles for Glioma Therapy

(2021) Wenbo Niu et al.   NANO LETTERS

Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells

(2021) Francesca Perut et al.   Biomolecules

Engineering Exosome-Like Nanovesicles Derived from Asparagus cochinchinensis Can Inhibit the Proliferation of Hepatocellular Carcinoma Cells with Better Safety Profile

(2021) Lei Zhang et al.   International Journal of Nanomedicine

Key principles and methods for studying the endocytosis of biological and nanoparticle therapeutics

(2021) Joshua J. Rennick et al.   Nature Nanotechnology

Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2

(2021) Sreeram Peringattu Kalarikkal et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Panax ginseng-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances

(2021) Eun-Gyung Cho et al.   Cells

Identification of anti‐inflammatory vesicle‐like nanoparticles in honey

(2021) Xingyi Chen et al.   Journal of Extracellular Vesicles

Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources

(2021) Eric Woith et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12

(2021) Yun Teng et al.   MOLECULAR THERAPY

A call for Rigor and standardization in plant extracellular vesicle research

(2021) Marcela Pinedo et al.   Journal of Extracellular Vesicles

Velocity Gradient Separation Reveals a New Extracellular Vesicle Population Enriched in miR-155 and Mitochondrial DNA

(2021) Myriam Vaillancourt et al.   Pathogens

Identification of Tomato Infecting Viruses That Co-Isolate with Nanovesicles Using a Combined Proteomics and Electron-Microscopic Approach

(2021) Ramila Mammadova et al.   Nanomaterials

Scaled preparation of extracellular vesicles from conditioned media

(2021) Simon Staubach et al.   ADVANCED DRUG DELIVERY REVIEWS

Effective methods for isolation and purification of extracellular vesicles from plants

(2021) Yifan Huang et al.   Journal of Integrative Plant Biology

Label‐free characterization of an extracellular vesicle‐based therapeutic

(2021) Eleni Priglinger et al.   Journal of Extracellular Vesicles

Topical treatment strategies to manipulate human skin pigmentation

(2020) Inbal Rachmin et al.   ADVANCED DRUG DELIVERY REVIEWS

Extracellular vesicles as drug delivery systems: Why and how?

(2020) Omnia M. Elsharkasy et al.   ADVANCED DRUG DELIVERY REVIEWS

A cost-effective polyethylene glycol-based method for the isolation of functional edible nanoparticles from ginger rhizomes

(2020) Sreeram Peringattu Kalarikkal et al.   Scientific Reports

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

(2020) Mariangela De Robertis et al.   Biomolecules

Lipidomic analysis reveals the importance of GIPCs in Arabidopsis leaf extracellular vesicles

(2020) Ning-Jing Liu et al.   Molecular Plant

Growing pains: addressing the pitfalls of plant extracellular vesicle research

(2020) Brian D. Rutter et al.   NEW PHYTOLOGIST

An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy

(2020) Meng Yang et al.   JOURNAL OF NANOBIOTECHNOLOGY

Impact of donation after circulatory death donor allografts on outcomes following liver transplantation for fulminant hepatic failure in the United States

(2020) Shiva Kumar et al.   AMERICAN JOURNAL OF TRANSPLANTATION

SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs

(2020) Qun Chen et al.   CELL RESEARCH

Dual‐Isolation and Profiling of Circulating Tumor Cells and Cancer Exosomes from Blood Samples with Melanoma Using Immunoaffinity‐Based Microfluidic Interfaces

(2020) Yoon‐Tae Kang et al.   Advanced Science

Internalization of Garlic-Derived Nanovesicles on Liver Cells is Triggered by Interaction With CD98

(2020) Heliang Song et al.   ACS Omega

Lemon Exosome-like Nanoparticles-Manipulated Probiotics Protect Mice from C. diff Infection

(2020) Chao Lei et al.   iScience

Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA

(2020) Meng Zhao et al.   ACS Nano

Plant Exosome-like Nanovesicles: Emerging Therapeutics and Drug Delivery Nanoplatforms

(2020) Haseeb Anwar Dad et al.   MOLECULAR THERAPY

Exosome-based candidates move into the clinic

(2020) Megan Cully   NATURE REVIEWS DRUG DISCOVERY

Comprehensive analysis of cutaneous and uveal melanoma liver metastases

(2020) Esmee P Hoefsmit et al.   Journal for ImmunoTherapy of Cancer

Extracellular Vesicles from Fresh and Dried Plants—Simultaneous Purification and Visualization Using Gel Electrophoresis

(2019) Eric Woith et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Oral Gavage of Ginger Nanoparticle-derived Lipid Vectors Loaded with Dmt1 siRNA Mitigates Iron Loading in Murine Hereditary Hemochromatosis

(2019) Xiaoyu Wang et al.   MOLECULAR THERAPY

Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization

(2019) Haiying Zhang et al.   Nature Protocols

Exosome-like Nanoparticles from Ginger Rhizomes Inhibited NLRP3 Inflammasome Activation

(2019) Xingyi Chen et al.   MOLECULAR PHARMACEUTICS

Turnip mosaic virus components are released into the extracellular space by vesicles in infected leaves

(2019) Nooshin Movahed et al.   PLANT PHYSIOLOGY

Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles

(2019) Phat Duong et al.   PLoS One

Plant extracellular vesicles

(2019) Yong Cui et al.   PROTOPLASMA

Exosomes in the phloem and xylem of woody plants

(2019) Dmitry G. Chukhchin et al.   PLANTA

Ginseng-derived nanoparticles alter macrophage polarization to inhibit melanoma growth

(2019) Meng Cao et al.   Journal for ImmunoTherapy of Cancer

Effects of Lyophilization of Arginine-rich Cell-penetrating Peptide-modified Extracellular Vesicles on Intracellular Delivery

(2019) KOSUKE NOGUCHI et al.   ANTICANCER RESEARCH

Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles

(2019) Christopher Stanly et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System

(2019) Oğuz Kaan Kırbaş et al.   Scientific Reports

Anti-melanogenic effects of extracellular vesicles derived from plant leaves and stems in mouse melanoma cells and human healthy skin

(2019) Ruri Lee et al.   Journal of Extracellular Vesicles

New Technologies for Analysis of Extracellular Vesicles

(2018) Huilin Shao et al.   CHEMICAL REVIEWS

Extracellular vesicles as key mediators of plant–microbe interactions

(2018) Brian D Rutter et al.   CURRENT OPINION IN PLANT BIOLOGY

Extracellular vesicles: a missing component in plant cell wall remodeling

(2018) Laura de la Canal et al.   JOURNAL OF EXPERIMENTAL BOTANY

Protein biocargo of citrus fruit juice sac cells-derived vesicles reveals heterogeneous transport and extracellular vesicle subpopulations

(2018) Gabriella Pocsfalvi et al.   JOURNAL OF PLANT PHYSIOLOGY

Technical challenges of working with extracellular vesicles

(2018) Marcel I. Ramirez et al.   Nanoscale

Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

(2018) Haiying Zhang et al.   NATURE CELL BIOLOGY

Shedding light on the cell biology of extracellular vesicles

(2018) Guillaume van Niel et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes

(2018) Qiang Cai et al.   SCIENCE

Quantifying Cellular Internalization with a Fluorescent Click Sensor

(2018) Laura I. Selby et al.   ACS Sensors

Plasma membrane aquaporins mediates vesicle stability in broccoli

(2018) Maria del Carmen Martínez-Ballesta et al.   PLoS One

Arrowtail RNA for Ligand Display on Ginger Exosome-like Nanovesicles to Systemic Deliver siRNA for Cancer Suppression

(2018) Zhefeng Li et al.   Scientific Reports

Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables

(2018) Juan Xiao et al.   PeerJ

Plant-Derived Exosomal MicroRNAs Shape the Gut Microbiota

(2018) Yun Teng et al.   Cell Host & Microbe

Exosome-like Nanovesicles Isolated from Citrus limon L. Exert Antioxidative Effect

(2018) Nicola Baldini et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Characterization of three different types of extracellular vesicles and their impact on bacterial growth

(2018) Siran Yu et al.   FOOD CHEMISTRY

Apple-Derived Nanoparticles Modulate Expression of Organic-Anion-Transporting Polypeptide (OATP) 2B1 in Caco-2 Cells

(2018) Daichi Fujita et al.   MOLECULAR PHARMACEUTICS

The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis

(2018) Daan Vorselen et al.   Nature Communications

Extracellular vesicles in food: Experimental evidence of their secretion in grape fruits

(2017) Pedro Pérez-Bermúdez et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth

(2017) Mariana Regente et al.   JOURNAL OF EXPERIMENTAL BOTANY

Broccoli-Derived Nanoparticle Inhibits Mouse Colitis by Activating Dendritic Cell AMP-Activated Protein Kinase

(2017) Zhongbin Deng et al.   MOLECULAR THERAPY

Oral administration of ginger-derived nanolipids loaded with siRNA as a novel approach for efficient siRNA drug delivery to treat ulcerative colitis

(2017) Mingzhen Zhang et al.   Nanomedicine

Extracellular vesicles in renal disease

(2017) Diana Karpman et al.   Nature Reviews Nephrology

Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7

(2017) Jianchao Du et al.   Science China-Life Sciences

Extracellular Vesicles: Unique Intercellular Delivery Vehicles

(2017) Sybren L.N. Maas et al.   TRENDS IN CELL BIOLOGY

Mechanistic understanding of in vivo protein corona formation on polymeric nanoparticles and impact on pharmacokinetics

(2017) Nicolas Bertrand et al.   Nature Communications

Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

(2017) Birke J. Benedikter et al.   Scientific Reports

Progress in Exosome Isolation Techniques

(2017) Pin Li et al.   Theranostics

Life Under the Microscope: Quantifying Live Cell Interactions to Improve Nanoscale Drug Delivery

(2017) Angus P. R. Johnston   ACS Sensors

Extracellular vesicles for drug delivery

(2016) Pieter Vader et al.   ADVANCED DRUG DELIVERY REVIEWS

Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer

(2016) Mingzhen Zhang et al.   BIOMATERIALS

Charge-based precipitation of extracellular vesicles

(2016) Maria Chiara Deregibus et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

DLS and zeta potential – What they are and what they are not?

(2016) Sourav Bhattacharjee   JOURNAL OF CONTROLLED RELEASE

Mesenchymal stem cells and their secreted molecules predominantly ameliorate fulminant hepatic failure and chronic liver fibrosis in mice respectively

(2016) Biao Huang et al.   Journal of Translational Medicine

Grapefruit-derived Nanovectors Delivering Therapeutic miR17 Through an Intranasal Route Inhibit Brain Tumor Progression

(2016) Xiaoying Zhuang et al.   MOLECULAR THERAPY

Edible Ginger-derived Nano-lipids Loaded with Doxorubicin as a Novel Drug-delivery Approach for Colon Cancer Therapy

(2016) Mingzhen Zhang et al.   MOLECULAR THERAPY

Extracellular Vesicles Isolated from the Leaf Apoplast Carry Stress-Response Proteins

(2016) Brian D. Rutter et al.   PLANT PHYSIOLOGY

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

(2016) Joanna Kowal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Grapefruit-derived nanovectors deliver miR-18a for treatment of liver metastasis of colon cancer by induction of M1 macrophages

(2016) Yun Teng et al.   Oncotarget

ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles

(2016) Mark A. Rider et al.   Scientific Reports

Size Characterization and Quantification of Exosomes by Asymmetrical-Flow Field-Flow Fractionation

(2015) Simona Sitar et al.   ANALYTICAL CHEMISTRY

Mass-Spectrometry-Based Molecular Characterization of Extracellular Vesicles: Lipidomics and Proteomics

(2015) Simion Kreimer et al.   JOURNAL OF PROTEOME RESEARCH

Mass spectrometry of extracellular vesicles

(2015) Gabriella Pocsfalvi et al.   MASS SPECTROMETRY REVIEWS

Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

(2015) Joel Z. Nordin et al.   Nanomedicine-Nanotechnology Biology and Medicine

Citrus limon-derived nanovesicles inhibit cancer cell proliferation and suppress CML xenograft growth by inducing TRAIL-mediated cell death

(2015) Stefania Raimondo et al.   Oncotarget

Dendritic cell-derived exosomes as maintenance immunotherapy after first line chemotherapy in NSCLC

(2015) Benjamin Besse et al.   OncoImmunology

Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses

(2014) Zhen Zhou et al.   CELL RESEARCH

Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood

(2014) Juliana Pessanha Rodrigues Motta et al.   CRYOBIOLOGY

A novel “salting-out” procedure for the isolation of tumor-derived exosomes

(2014) Zachary Brownlee et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles

(2014) Jingyao Mu et al.   MOLECULAR NUTRITION & FOOD RESEARCH

Endophilin marks and controls a clathrin-independent endocytic pathway

(2014) Emmanuel Boucrot et al.   NATURE

The Plant Endomembrane System—A Complex Network Supporting Plant Development and Physiology

(2014) Miyo Terao Morita et al.   PLANT AND CELL PHYSIOLOGY

A Programmable Sensor to Probe the Internalization of Proteins and Nanoparticles in Live Cells

(2013) Haiyin Liu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Integration of Chemical and RNAi Multiparametric Profiles Identifies Triggers of Intracellular Mycobacterial Killing

(2013) Varadharajan Sundaramurthy et al.   Cell Host & Microbe

Grape Exosome-like Nanoparticles Induce Intestinal Stem Cells and Protect Mice From DSS-Induced Colitis

(2013) Songwen Ju et al.   MOLECULAR THERAPY

Targeted Drug Delivery to Intestinal Macrophages by Bioactive Nanovesicles Released from Grapefruit

(2013) Baomei Wang et al.   MOLECULAR THERAPY

Extracellular vesicles: biology and emerging therapeutic opportunities

(2013) Samir EL Andaloussi et al.   NATURE REVIEWS DRUG DISCOVERY

Fungal Small RNAs Suppress Plant Immunity by Hijacking Host RNA Interference Pathways

(2013) Arne Weiberg et al.   SCIENCE

Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids

(2013) Qilong Wang et al.   Nature Communications

Purification of DNA-origami nanostructures by rate-zonal centrifugation

(2012) Chenxiang Lin et al.   NUCLEIC ACIDS RESEARCH

Treatment of Brain Inflammatory Diseases by Delivering Exosome Encapsulated Anti-inflammatory Drugs From the Nasal Region to the Brain

(2011) Xiaoying Zhuang et al.   MOLECULAR THERAPY

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis

(2011) Rebecca A. Dragovic et al.   Nanomedicine-Nanotechnology Biology and Medicine

Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes

(2011) Lydia Alvarez-Erviti et al.   NATURE BIOTECHNOLOGY

Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size

(2011) H. Cabral et al.   Nature Nanotechnology

The safety and side effects of monoclonal antibodies

(2010) Trevor T. Hansel et al.   NATURE REVIEWS DRUG DISCOVERY

EXPO, an Exocyst-Positive Organelle Distinct from Multivesicular Endosomes and Autophagosomes, Mediates Cytosol to Cell Wall Exocytosis inArabidopsisand Tobacco Cells

(2010) Juan Wang et al.   PLANT CELL

Novel test for microparticles in platelet-rich plasma and platelet concentrates using dynamic light scattering

(2010) Yan Xu et al.   TRANSFUSION

Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins

(2009) Mariana Regente et al.   FEBS LETTERS

A novel membrane fusion-mediated plant immunity against bacterial pathogens

(2009) N. Hatsugai et al.   GENES & DEVELOPMENT

Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular Injury

(2009) S. Bruno et al.   JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Microparticle sizing by dynamic light scattering in fresh-frozen plasma

(2009) A. S. Lawrie et al.   VOX SANGUINIS

Phospholipids are present in extracellular fluids of imbibing sunflower seeds and are modulated by hormonal treatments

(2008) Mariana Regente et al.   JOURNAL OF EXPERIMENTAL BOTANY

Extracellular transport and integration of plant secretory proteins into pathogen-induced cell wall compartments

(2008) Dorit Meyer et al.   PLANT JOURNAL