Article
Biotechnology & Applied Microbiology
Xue Yao, Baixue Liao, Feng Chen, Lueye Liu, Kaiwen Wu, Yaying Hao, Yanping Li, Yuebin Wang, Ruiling Fan, Jun Yin, Lei Liu, Yuanbiao Guo
Summary: By comparing three different methods for isolating extracellular vesicles from pleural effusion (lung cancer patients), it was found that the combination of ultracentrifugation and size exclusion chromatography (UC-SEC) yielded the highest purity of vesicles. CD11C, HLA DPA1, and HLA DRB1 were identified as potential protein markers for pleural effusion-derived vesicles (pEVs), providing important information for studying diseases complicated with pleural effusion.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Simona Serrati, Antonio Palazzo, Annamaria Lapenna, Helena Mateos, Antonia Mallardi, Rene Massimiliano Marsano, Alessandra Quarta, Mario Del Rosso, Amalia Azzariti
Summary: The role of extracellular vesicles in intercellular communication has been re-evaluated, and developing faster, cheaper isolation protocols for EVs is crucial. The study found that salting-out method was more efficient in EV separation and protein depletion than ultracentrifugation, highlighting it as a potential alternative method.
Review
Biotechnology & Applied Microbiology
Sun Jiawei, Chen Zhi, Tian Kewei, Li Xiaoping
Summary: In this study, commonly used exosome isolation approaches are reviewed, with a focus on magnetic bead-based capture, which offers high purity and integrity of exosomes. The challenges of traditional methods for exosome isolation are also described, highlighting the directions for future research and development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marina Alorda-Clara, Jose Reyes, Marita Grimanesa Trelles-Guzman, Monica Florido, Pilar Roca, Daniel Gabriel Pons, Jordi Oliver
Summary: Colorectal cancer is the third most common cancer worldwide, and the lack of early and specific biomarkers leads to late-stage detection. Tumors release extracellular vesicles (EVs) that have various functions and can be used as a source of biomarkers. Bowel lavage fluid (BLF) obtained during colonoscopy is an underutilized sample that represents EVs from tumor cells and has the potential to be a useful research tool. In this study, EVs from BLF were isolated and characterized, confirming their suitability for CRC study and potential as biomarkers for diagnosis and monitoring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maria Soares, Maria M. Pinto, Rui Jorge Nobre, Luis Pereira de Almeida, Maria da Graca Rasteiro, Teresa Almeida-Santos, Joao Ramalho-Santos, Ana Paula Sousa
Summary: This study compared the efficacy and purity of two methods for isolating extracellular vesicles (EVs): size-exclusion chromatography (SEC) and ultracentrifugation (UC). The results showed that UC yielded a significantly higher number of EVs, while SEC produced purer EVs.
Article
Biochemical Research Methods
Meng Li, Doudou Lou, Joyce Chen, Keqing Shi, Yong Wang, Qingfu Zhu, Fei Liu, Yating Zhang
Summary: Salivary extracellular vesicles obtained by ultracentrifugation and polyethylene glycol precipitation methods were compared in this study, with the former showing higher expression of EV-specific markers. Analysis using tandem mass spectrometry proteomics revealed that ultracentrifugation can isolate more EV-related proteins, providing guidance for EV separation and potential applications in non-invasive diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Migmar Tsamchoe, Stephanie Petrillo, Anthoula Lazaris, Peter Metrakos
Summary: This study compared the effects of different methods for extracellular vesicle (EV) isolation and found that appropriate controls are necessary when adding any additives during EV isolation, as even minor contaminants can have a significant impact on results. Additionally, it was found that biobanked plasma can be used for EV isolation with no major alterations needed.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Cell & Tissue Engineering
Soraya Williams, Maria Fernandez-Rhodes, Alice Law, Ben Peacock, Mark P. Lewis, Owen G. Davies
Summary: The lack of optimal isolation methods hampers the clinical translation of extracellular vesicles (EVs) despite their increasing interest for therapeutic applications. This study evaluated the impact of commonly used isolation methods on EV purity and yield, revealing variations in purity and surface marker expression among different methods.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Juan Alfonso Martinez-Greene, Margarita Gomez-Chavarin, Maria del Pilar Ramos-Godinez, Eduardo Martinez-Martinez
Summary: In recent years, the study of extracellular vesicles (EVs) in the context of various diseases has increased due to their diagnostic and therapeutic potential. New methods for isolating EVs directly from tissue samples have been developed to better represent the in vivo microenvironment. This study established a protocol for isolating EVs from hepatic and adipose tissue of mice, allowing for a more precise understanding of their molecular composition and functions in intercellular communication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Valentina Moccia, Cinzia Centelleghe, Ilaria Giusti, Antonella Peruffo, Vincenza Dolo, Sandro Mazzariol, Valentina Zappulli
Summary: In this study, extracellular vesicles (EVs) were isolated from a bottlenose dolphin and a Cuvier's beaked whale cell line using two different techniques. The EVs were characterized using nanoparticle tracking analysis, Western blotting, and scanning transmission electron microscopy. Further research on cell-derived EVs will enhance our understanding of cetacean pathophysiology and health status assessment.
Article
Biotechnology & Applied Microbiology
Maria Isabel Gonzalez, Begona Gallardo, Carlos Ceron, Elena Aguilera-Jimenez, Marta Cortes-Canteli, Hector Peinado, Manuel Desco, Beatriz Salinas
Summary: This study presents an optimized approach for purifying exosomes from goat milk by combining physical isolation methods with biological treatment. The vesicles isolated with this novel strategy showed characteristics similar to exosomes and high homogeneity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Fisheries
Chamilani Nikapitiya, Eriyawala Hewage Thimira Thulshan Jayathilaka, Shan Lakmal Edirisinghe, Dinusha C. Rajapaksha, Withanage Prasadini Wasana, Jayasinghage Nirmani Chathurangika Jayasinghe, Mahanama De Zoysa
Summary: This study isolated exosomes from the plasma of rock bream using differential ultracentrifugation and a commercial membrane affinity spin column technique. The kit-based method was found to be more suitable for isolating high-purity exosomes, while UC method showed higher safety in terms of yielding exosomes with low toxicity.
Article
Microbiology
Rachel R. Mizenko, Terza Brostoff, Kenneth Jackson, Patricia A. Pesavento, Randy P. Carney
Summary: Feline calicivirus (FCV) is a significant cause of respiratory disease in cats and has implications for human medical research. This study explores the role of extracellular vesicles (EVs) in FCV infection and highlights the importance of coisolates during viral purification.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Laura Saludas, Elisa Garbayo, Adrian Ruiz-Villalba, Silvia Hernandez, Pieter Vader, Felipe Prosper, Maria J. Blanco-Prieto
Summary: Extracellular vesicles (EVs), as the next-generation therapies, have attracted great interest. This study investigated the effects of different cell culture conditions and purification methods on EV isolation using cardiac progenitor cells (CPCs). The findings contribute to the establishment of the basis for the use of EVs as therapeutic platforms.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Junli Zhang, Yifan Zhu, Mengting Guan, Yingying Liu, Min Lv, Chongwei Zhang, Hongling Zhang, Zhenzhong Zhang
Summary: Exosomes have potential as cancer biomarkers in clinical applications, but enrichment and detection from complex media remain challenging. This study used iodixanol density gradient centrifugation for exosome isolation and purification, and surface plasmon resonance (SPR) biochip for label-free detection of exosomal PD-L1. The method showed better specificity in distinguishing exosomes with different levels of PD-L1 compared to enzyme-linked immunosorbent assays.
Article
Immunology
Laura Bouchareychas, Eva M. Groessinger, Mincheol Kang, Hong Qiu, Iannis E. Adamopoulos
JOURNAL OF IMMUNOLOGY
(2017)
Article
Endocrinology & Metabolism
S. Beliard, W. Le Goff, F. Saint-Charles, L. Poupel, V. Deswaerte, L. Bouchareychas, T. Huby, P. Lesnik
INTERNATIONAL JOURNAL OF OBESITY
(2017)
Article
Immunology
Eva M. Grossinger, Mincheol Kang, Laura Bouchareychas, Ritu Sarin, Dominik R. Haudenschild, Laura N. Borodinsky, Iannis E. Adamopoulos
JOURNAL OF IMMUNOLOGY
(2018)
Article
Immunology
Yehudi Bloch, Laura Bouchareychas, Romain Merceron, Katarzyna Skladanowska, Lien Van den Bossche, Sammy Detry, Srinath Govindarajan, Dirk Elewaut, Filomeen Haerynck, Melissa Dullaers, Iannis E. Adamopoulos, Savvas N. Savvides
Article
Multidisciplinary Sciences
Laura Bouchareychas, Eva M. Grossinger, Mincheol Kang, Iannis E. Adamopoulos
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Oscar D. Murillo, William Thistlethwaite, Joel Rozowsky, Sai Lakshmi Subramanian, Rocco Lucero, Neethu Shah, Andrew R. Jackson, Srimeenakshi Srinivasan, Allen Chung, Clara D. Laurent, Robert R. Kitchen, Timur Galeev, Jonathan Warrell, James A. Diao, Joshua A. Welsh, Kristina Hanspers, Anders Riutta, Sebastian Burgstaller-Muehlbacher, Ravi Shah, Ashish Yeri, Lisa M. Jenkins, Mehmet E. Ahsen, Carlos Cordon-Cardo, Navneet Dogra, Stacey M. Gifford, Joshua T. Smith, Gustavo Stolovitzky, Ashutosh K. Tewari, Benjamin H. Wunsch, Kamlesh K. Yadav, Kirsty M. Danielson, Justyna Filant, Courtney Moeller, Parham Nejad, Anu Paul, Bridget Simonson, David K. Wong, Xuan Zhang, Leonora Balaj, Roopali Gandhi, Anil K. Sood, Roger P. Alexander, Liang Wang, Chunlei Wu, David T. W. Wong, David J. Galas, Kendall van Keuren-Jensen, Tushar Patel, Jennifer C. Jones, Saumya Das, Kei-Hoi Cheung, Alexander R. Pico, Andrew Su, Robert L. Raffai, Louise C. Laurent, Matthew E. Roth, Mark B. Gerstein, Aleksandar Milosavljevic
Article
Biochemistry & Molecular Biology
Srimeenakshi Srinivasan, Ashish Yeri, Pike See Cheah, Allen Chung, Kirsty Danielson, Peter De Hoff, Justyna Filant, Clara D. Laurent, Lucie D. Laurent, Rogan Magee, Courtney Moeller, Venkatesh L. Murthy, Parham Nejad, Anu Paul, Isidore Rigoutsos, Rodosthenis Rodosthenous, Ravi Shah, Bridget Simonson, Cuong To, David Wong, Irene K. Yan, Xuan Zhang, Leonora Balaj, Xandra O. Breakefield, George Daaboul, Roopali Gandhi, Jodi Lapidus, Eric Londin, Tushar Patel, Robert L. Raffai, Anil K. Sood, Roger P. Alexander, Saumya Das, Louise C. Laurent
Article
Cell Biology
Laura Bouchareychas, Phat Duong, Sergio Covarrubias, Eric Alsop, Tuan Anh Phu, Allen Chung, Michael Gomes, David Wong, Bessie Meechoovet, Allyson Capili, Ryo Yamamoto, Hiromitsu Nakauchi, Michael T. McManus, Susan Carpenter, Kendall Van Keuren-Jensen, Robert L. Raffai
Article
Multidisciplinary Sciences
Laura Bouchareychas, Phat Duong, Tuan Anh Phu, Eric Alsop, Bessie Meechoovet, Rebecca Reiman, Martin Ng, Ryo Yamamoto, Hiromitsu Nakauchi, Warren J. Gasper, Kendall Van Keuren-Jensen, Robert L. Raffai
Summary: The study showed that extracellular vesicles (EVs) produced under hyperglycemic conditions can accelerate atherosclerosis in diabetes by increasing hematopoiesis, changing metabolic activity, inducing cell proliferation, and reprogramming energy metabolism. Profiles of microRNA in EVs from diabetic subjects with advanced atherosclerosis identified miR-486-5p as a common factor in dysregulated hematopoiesis and Abca1 control.
Review
Peripheral Vascular Disease
Allen Chung, Muredach P. Reilly, Robert C. Bauer
Summary: This review focuses on the genetic association between ADAMTS7 and coronary artery disease, the functional studies of ADAMTS7 in mouse models, and the potential substrates of ADAMTS7. Recent studies have confirmed the genetic link between ADAMTS7 and atherosclerosis and showed that the proatherogenicity of ADAMTS7 is derived from its catalytic functions. Furthermore, vaccination against ADAMTS7 reduced atherosclerosis in mice, suggesting that ADAMTS7 could be a target for therapeutic intervention.
CURRENT ATHEROSCLEROSIS REPORTS
(2023)
Review
Cardiac & Cardiovascular Systems
Laura Bouchareychas, Robert L. Raffai
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2018)
Meeting Abstract
Biophysics
Eva M. Grossinger, Mincheol Kang, Laura Bouchareychas, Ritu Sarin, Dominik Haudenschild, Laura Borodinsky, Iannis E. Adamopoulos
BIOPHYSICAL JOURNAL
(2017)