Deconstructing virus condensation

Formation and Function of Liquid-Like Viral Factories in Negative-Sense Single-Stranded RNA Virus Infections

(2021) Justin M. Su et al.   Viruses-Basel

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

(2021) Shan Lu et al.   Nature Communications

Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation

(2021) Olga Vladimirova et al.   PLoS Pathogens

SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production

(2021) Lingling Luo et al.   Science Bulletin

ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain

(2021) Mei Dang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Pneumoviral Phosphoprotein, a Multidomain Adaptor-Like Protein of Apparent Low Structural Complexity and High Conformational Versatility

(2021) Christophe Cardone et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Ligand effects on phase separation of multivalent macromolecules

(2021) Kiersten M. Ruff et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The HSV-1 Transcription Factor ICP4 Confers Liquid-Like Properties to Viral Replication Compartments

(2021) Michael Seyffert et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ATP regulates RNA ‐driven CIRBP phase separation

(2021) Qishun Zhou et al.   PROTEIN SCIENCE

GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein

(2021) Ming Zhao et al.   Nature Communications

The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA

(2021) Jasmine Cubuk et al.   Nature Communications

A condensate-hardening drug blocks RSV replication in vivo

(2021) Jennifer Risso-Ballester et al.   NATURE

Targeting liquid–liquid phase separation of SARS-CoV-2 nucleocapsid protein promotes innate antiviral immunity by elevating MAVS activity

(2021) Shuai Wang et al.   NATURE CELL BIOLOGY

Mechanistic dissection of increased enzymatic rate in a phase-separated compartment

(2021) William Peeples et al.   Nature Chemical Biology

Liquid–Liquid Phase Separation in Chromatin

(2021) Karsten Rippe   Cold Spring Harbor Perspectives in Biology

Tombusviruses orchestrate the host endomembrane system to create elaborate membranous replication organelles

(2021) Peter D Nagy et al.   Current Opinion in Virology

A Guide to Regulation of the Formation of Biomolecular Condensates

(2020) Anna Bratek‐Skicki et al.   FEBS Journal

Phase separation of Epstein-Barr Virus EBNA2 and Its Coactivator EBNALP controls gene expression

(2020) Qiu Peng et al.   JOURNAL OF VIROLOGY

Replication Compartments of DNA Viruses in the Nucleus: Location, Location, Location

(2020) Matthew Charman et al.   Viruses-Basel

Conserved Outer Tegument Component UL11 from Herpes Simplex Virus 1 Is an Intrinsically Disordered, RNA-Binding Protein

(2020) Claire M. Metrick et al.   mBio

Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking

(2020) Anne Monette et al.   Cell Reports

Ebola Virus Inclusion Body Formation and RNA Synthesis Are Controlled by a Novel Domain of Nucleoprotein Interacting with VP35

(2020) Tsuyoshi Miyake et al.   JOURNAL OF VIROLOGY

Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder

(2020) Lovisa Stenström et al.   Molecular Systems Biology

α-Synuclein aggregation nucleates through liquid–liquid phase separation

(2020) Soumik Ray et al.   Nature Chemistry

A molecular pore spans the double membrane of the coronavirus replication organelle

(2020) Georg Wolff et al.   SCIENCE

Nucleocapsid Structure of Negative Strand RNA Virus

(2020) Ming Luo et al.   Viruses-Basel

The RNA Replication Site of Tula Orthohantavirus Resides within a Remodelled Golgi Network

(2020) Katherine A. Davies et al.   Cells

The emergence of phase separation as an organizing principle in bacteria

(2020) Christopher A. Azaldegui et al.   BIOPHYSICAL JOURNAL

Mammalian stress granules and P bodies at a glance

(2020) Claire L. Riggs et al.   JOURNAL OF CELL SCIENCE

Nucleated transcriptional condensates amplify gene expression

(2020) Ming-Tzo Wei et al.   NATURE CELL BIOLOGY

The nucleolus as a multiphase liquid condensate

(2020) Denis L. J. Lafontaine et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Components and Architecture of the Rhabdovirus Ribonucleoprotein Complex

(2020) Christiane Riedel et al.   Viruses-Basel

Dance with the Devil: Stress Granules and Signaling in Antiviral Responses

(2020) Nina Eiermann et al.   Viruses-Basel

Minimal Elements Required for the Formation of Respiratory Syncytial Virus Cytoplasmic Inclusion Bodies In Vivo and In Vitro

(2020) Marie Galloux et al.   mBio

ssRNA Virus and Host Lipid Rearrangements: Is There a Role for Lipid Droplets in SARS-CoV-2 Infection?

(2020) Francesca Pagliari et al.   Frontiers in Molecular Biosciences

Negri bodies and other virus membrane-less replication compartments

(2020) Quentin Nevers et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

(2020) Theodora Myrto Perdikari et al.   EMBO JOURNAL

Clustering and reverse transcription of HIV‐1 genomes in nuclear niches of macrophages

(2020) Elena Rensen et al.   EMBO JOURNAL

Liquid–Liquid Phase Separation by Intrinsically Disordered Protein Regions of Viruses: Roles in Viral Life Cycle and Control of Virus–Host Interactions

(2020) Stefania Brocca et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions

(2020) Christopher R. Carlson et al.   MOLECULAR CELL

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

(2020) Christiane Iserman et al.   MOLECULAR CELL

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

(2020) Adriana Savastano et al.   Nature Communications

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

(2020) Steffen Klein et al.   Nature Communications

Lipid droplets fuel SARS-CoV-2 replication and production of inflammatory mediators

(2020) Suelen Silva Gomes Dias et al.   PLoS Pathogens

Insights into gene regulation: From regulatory genomic elements to DNA-protein and protein-protein interactions

(2020) Leonid Serebreni et al.   CURRENT OPINION IN CELL BIOLOGY

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Coalescing replication compartments provide the opportunity for recombination between coinfecting herpesviruses

(2019) Enosh Tomer et al.   FASEB JOURNAL

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

Temperature, Hydrostatic Pressure, and Osmolyte Effects on Liquid-Liquid Phase Separation in Protein Condensates: Physical Chemistry and Biological Implications

(2019) Roland Hermann Alfons Winter et al.   CHEMISTRY-A EUROPEAN JOURNAL

Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

(2019) Marta Alenquer et al.   Nature Communications

Nanoscale organization of rotavirus replication machineries

(2019) Yasel Garcés Suárez et al.   eLife

Liquid–Liquid Phase Separation in Disease

(2019) Simon Alberti et al.   Annual Review of Genetics

Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles

(2019) Yuqin Zhou et al.   JOURNAL OF VIROLOGY

Formation of adenovirus DNA replication compartments

(2019) Paloma Hidalgo et al.   FEBS LETTERS

Coronavirus genomic RNA packaging

(2019) Paul S. Masters   VIROLOGY

Taxonomy of the order Mononegavirales: update 2018

(2018) Gaya K. Amarasinghe et al.   ARCHIVES OF VIROLOGY

Cytoplasmic Relocalization and Colocalization with Viroplasms of Host Cell Proteins, and Their Role in Rotavirus Infection

(2018) Poonam Dhillon et al.   JOURNAL OF VIROLOGY

Rewiring cellular networks by members of the Flaviviridae family

(2018) Christopher J. Neufeldt et al.   NATURE REVIEWS MICROBIOLOGY

Controlling compartmentalization by non-membrane-bound organelles

(2018) Richard J. Wheeler et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

(2018) Won-Ki Cho et al.   SCIENCE

Organization and Function of Non-dynamic Biomolecular Condensates

(2018) Jeffrey B. Woodruff et al.   TRENDS IN BIOCHEMICAL SCIENCES

Paraspeckles: Where Long Noncoding RNA Meets Phase Separation

(2018) Archa H. Fox et al.   TRENDS IN BIOCHEMICAL SCIENCES

Self-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation

(2018) Diana M. Mitrea et al.   Nature Communications

RSV hijacks cellular protein phosphatase 1 to regulate M2-1 phosphorylation and viral transcription

(2018) Charles-Adrien Richard et al.   PLoS Pathogens

Multiple Modes of Protein–Protein Interactions Promote RNP Granule Assembly

(2018) Tanja Mittag et al.   JOURNAL OF MOLECULAR BIOLOGY

Methods for Physical Characterization of Phase-Separated Bodies and Membrane-less Organelles

(2018) Diana M. Mitrea et al.   JOURNAL OF MOLECULAR BIOLOGY

Phase Transitions Drive the Formation of Vesicular Stomatitis Virus Replication Compartments

(2018) Bianca S. Heinrich et al.   mBio

Human Metapneumovirus Induces Formation of Inclusion Bodies for Efficient Genome Replication and Transcription

(2017) Nicolás Cifuentes-Muñoz et al.   JOURNAL OF VIROLOGY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Negri bodies are viral factories with properties of liquid organelles

(2017) Jovan Nikolic et al.   Nature Communications

Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus

(2017) Vincent Rincheval et al.   Nature Communications

Ultrastructural Characterization of Zika Virus Replication Factories

(2017) Mirko Cortese et al.   Cell Reports

RNA assemblages orchestrate complex cellular processes

(2016) Finn Cilius Nielsen et al.   BIOESSAYS

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

Tandemly Integrated HPV16 Can Form a Brd4-Dependent Super-Enhancer-Like Element That Drives Transcription of Viral Oncogenes

(2016) Katharine E. Dooley et al.   mBio

Moving On Out: Transport and Packaging of Influenza Viral RNA into Virions

(2016) Seema S. Lakdawala et al.   Annual Review of Virology

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Relating sequence encoded information to form and function of intrinsically disordered proteins

(2015) Rahul K Das et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Initiation and regulation of paramyxovirus transcription and replication

(2015) Sarah L. Noton et al.   VIROLOGY

Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules

(2015) Sonja Kroschwald et al.   eLife

In Vitro Reconstitution of a Cellular Phase-Transition Process that Involves the mRNA Decapping Machinery

(2014) Simon A. Fromm et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Nucleocapsid Phosphorylation and RNA Helicase DDX1 Recruitment Enables Coronavirus Transition from Discontinuous to Continuous Transcription

(2014) Chia-Hsin Wu et al.   Cell Host & Microbe

Roles of viroplasm-like structures formed by nonstructural protein NSs in infection with severe fever with thrombocytopenia syndrome virus

(2014) Xiaodong Wu et al.   FASEB JOURNAL

Viral and cellular subnuclear structures in human cytomegalovirus-infected cells

(2014) B. L. Strang   JOURNAL OF GENERAL VIROLOGY

Focal Adhesion Kinase Is Involved in Rabies Virus Infection through Its Interaction with Viral Phosphoprotein P

(2014) Baptiste Fouquet et al.   JOURNAL OF VIROLOGY

Paramyxovirus Glycoprotein Incorporation, Assembly and Budding: A Three Way Dance for Infectious Particle Production

(2014) Farah El Najjar et al.   Viruses-Basel

Bromovirus-induced remodeling of host membranes during viral RNA replication

(2014) Arturo Diaz et al.   Current Opinion in Virology

Translation Repressors, an RNA Helicase, and Developmental Cues Control RNP Phase Transitions during Early Development

(2013) Arnaud Hubstenberger et al.   DEVELOPMENTAL CELL

Cellular Chaperonin CCT  Contributes to Rabies Virus Replication during Infection

(2013) J. Zhang et al.   JOURNAL OF VIROLOGY

Measles Virus Nonstructural C Protein Modulates Viral RNA Polymerase Activity by Interacting with Host Protein SHCBP1

(2013) M. Ito et al.   JOURNAL OF VIROLOGY

Analysis of Borna Disease Virus Trafficking in Live Infected Cells by Using a Virus Encoding a Tetracysteine-Tagged P Protein

(2013) C. M. Charlier et al.   JOURNAL OF VIROLOGY

Poxvirus DNA Replication

(2013) B. Moss   Cold Spring Harbor Perspectives in Biology

Colocalization of Different Influenza Viral RNA Segments in the Cytoplasm before Viral Budding as Shown by Single-molecule Sensitivity FISH Analysis

(2013) Yi-ying Chou et al.   PLoS Pathogens

The Spatial Organization of DNA Virus Genomes in the Nucleus

(2013) Roger D. Everett   PLoS Pathogens

Bornavirus Closely Associates and Segregates with Host Chromosomes to Ensure Persistent Intranuclear Infection

(2012) Yusuke Matsumoto et al.   Cell Host & Microbe

Arenavirus Infection Induces Discrete Cytosolic Structures for RNA Replication

(2012) N. L. Baird et al.   JOURNAL OF VIROLOGY

Visualizing HIV-1 Assembly

(2011) Nolwenn Jouvenet et al.   JOURNAL OF MOLECULAR BIOLOGY

Complex Dynamic Development of Poliovirus Membranous Replication Complexes

(2011) G. A. Belov et al.   JOURNAL OF VIROLOGY

The Papillomavirus E1 Helicase Activates a Cellular DNA Damage Response in Viral Replication Foci

(2011) N. Sakakibara et al.   JOURNAL OF VIROLOGY

Ultrastructural Characterization of Arterivirus Replication Structures: Reshaping the Endoplasmic Reticulum To Accommodate Viral RNA Synthesis

(2011) K. Knoops et al.   JOURNAL OF VIROLOGY

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA

(2011) L. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rotaviruses Associate with Cellular Lipid Droplet Components To Replicate in Viroplasms, and Compounds Disrupting or Blocking Lipid Droplets Inhibit Viroplasm Formation and Viral Replication

(2010) W. Cheung et al.   JOURNAL OF VIROLOGY

Viral Disorder or Disordered Viruses: Do Viral Proteins Possess Unique Features?

(2010) Bin Xue et al.   PROTEIN AND PEPTIDE LETTERS

The Human Papillomavirus E7−E2 Interaction Mechanism in Vitro Reveals a Finely Tuned System for Modulating Available E7 and E2 Proteins

(2009) Clara Smal et al.   BIOCHEMISTRY

Modular Organization of Rabies Virus Phosphoprotein

(2009) Francine C.A. Gerard et al.   JOURNAL OF MOLECULAR BIOLOGY

The Respiratory Syncytial Virus M2-1 Protein Forms Tetramers and Interacts with RNA and P in a Competitive Manner

(2009) T.-L. Tran et al.   JOURNAL OF VIROLOGY

Functional Characterization of Negri Bodies (NBs) in Rabies Virus-Infected Cells: Evidence that NBs Are Sites of Viral Transcription and Replication

(2009) X. Lahaye et al.   JOURNAL OF VIROLOGY

Dengue Virus Capsid Protein Usurps Lipid Droplets for Viral Particle Formation

(2009) Marcelo M. Samsa et al.   PLoS Pathogens

Endoplasmic Reticulum Chaperones Are Involved in the Morphogenesis of Rotavirus Infectious Particles

(2008) L. Maruri-Avidal et al.   JOURNAL OF VIROLOGY

SARS-Coronavirus Replication Is Supported by a Reticulovesicular Network of Modified Endoplasmic Reticulum

(2008) Kèvin Knoops et al.   PLOS BIOLOGY