Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9

PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways

(2021) Mingming Qi et al.   Frontiers in Bioengineering and Biotechnology

CDK1 inhibitor controls G2/M phase transition and reverses DNA damage sensitivity

(2021) Shigeaki Sunada et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

High-yield production of (R)-acetoin in Saccharomyces cerevisiae by deleting genes for NAD(P)H-dependent ketone reductases producing meso-2,3-butanediol and 2,3-dimethylglycerate

(2021) Sang-Jeong Bae et al.   METABOLIC ENGINEERING

Production of (2R,3R)-butanediol by Paenibacillus polymyxa PM 3605 from crude glycerol supplemented with sugarcane molasses

(2021) Daniel Tinôco et al.   PROCESS BIOCHEMISTRY

Yeast synthetic biology for designed cell factories producing secretory recombinant proteins

(2020) Eun Jung Thak et al.   FEMS YEAST RESEARCH

Metabolic engineering of Pichia pastoris for production of isopentanol (3-Methyl-1-butanol)

(2020) Wiparat Siripong et al.   ENZYME AND MICROBIAL TECHNOLOGY

High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris

(2020) Yankun Yang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks

(2020) Tai-Ting Woo et al.   NUCLEIC ACIDS RESEARCH

Identifying improved sites for heterologous gene integration using ATAC-seq

(2020) Joseph R Brady et al.   ACS Synthetic Biology

Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli

(2020) Yonggang Wang et al.   PeerJ

A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae

(2020) Chang Dong et al.   ACS Synthetic Biology

Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

(2020) Yoichiro Ito et al.   NUCLEIC ACIDS RESEARCH

Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris

(2019) Yang Gu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Methods for Enhancing Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Homology-Directed Repair Efficiency

(2019) Xi-Dian Tang et al.   Frontiers in Genetics

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

(2019) Qi Liu et al.   Microbial Cell Factories

Advances in engineering methylotrophic yeast for biosynthesis of valuable chemicals from methanol

(2018) Xingpeng Duan et al.   CHINESE CHEMICAL LETTERS

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris

(2018) Ning Xu et al.   YEAST

5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger

(2018) Xiaomei Zheng et al.   ACS Synthetic Biology

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Enhancing the production of recombinant acidic α-amylase and phytase in Pichia pastoris under dual promoters [constitutive ( GAP ) and inducible ( AOX )] in mixed fed batch high cell density cultivation

(2016) Deepak Parashar et al.   PROCESS BIOCHEMISTRY

Metabolic engineering of Pichia pastoris for the production of dammarenediol-II

(2015) Xin-Bin Liu et al.   JOURNAL OF BIOTECHNOLOGY

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol

(2014) Jiazhang Lian et al.   METABOLIC ENGINEERING

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

(2013) Limei Gao et al.   Microbial Cell Factories

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

A color-based stable multi-copy integrant selection system forPichia pastorisusing the attenuatedADE1andADE2genes as auxotrophic markers

(2012) Min Du et al.   Bioengineered

Expression of GFP usingPichia pastorisvectors with zeocin or G-418 sulphate as the primary selectable marker

(2009) Theo Papakonstantinou et al.   YEAST