PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways

CRISPR/Cas13d-Mediated Microbial RNA Knockdown

(2020) Kun Zhang et al.   Frontiers in Bioengineering and Biotechnology

Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

(2019) Jiazhang Lian et al.   Nature Communications

Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

(2018) Johanna Hausjell et al.   BIOSCIENCE REPORTS

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains

(2018) Jiazhang Lian et al.   BIOTECHNOLOGY AND BIOENGINEERING

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

(2018) Jiazhang Lian et al.   Biotechnology Journal

Recent advances in metabolic engineering of Saccharomyces cerevisiae : New tools and their applications

(2018) Jiazhang Lian et al.   METABOLIC ENGINEERING

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

(2018) Jiazhang Lian et al.   Biotechnology Journal

A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast

(2018) Leanne Bourgeois et al.   ACS Synthetic Biology

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

(2016) Jiazhang Lian et al.   BIOTECHNOLOGY AND BIOENGINEERING

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

(2016) Mathew M Jessop-Fabre et al.   Biotechnology Journal

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

(2016) Shuobo Shi et al.   METABOLIC ENGINEERING

A Cas9-based toolkit to program gene expression inSaccharomyces cerevisiae

(2016) Amanda Reider Apel et al.   NUCLEIC ACIDS RESEARCH

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

(2016) Mathew M Jessop-Fabre et al.   Biotechnology Journal

High-throughput functional genomics using CRISPR–Cas9

(2015) Ophir Shalem et al.   NATURE REVIEWS GENETICS

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

(2015) Tadas Jakočiu̅nas et al.   ACS Synthetic Biology

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Reversal of the β-Oxidation Cycle in Saccharomyces cerevisiae for Production of Fuels and Chemicals

(2014) Jiazhang Lian et al.   ACS Synthetic Biology

Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications

(2013) Kathleen A. Curran et al.   METABOLIC ENGINEERING

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae

(2012) Jie Sun et al.   BIOTECHNOLOGY AND BIOENGINEERING

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae

(2011) Nancy A. Da Silva et al.   FEMS YEAST RESEARCH

Stabilized gene duplication enables long-term selection-free heterologous pathway expression

(2009) Keith E J Tyo et al.   NATURE BIOTECHNOLOGY

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH