Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

Design and construction of short synthetic terminators for β-amyrin production in Saccharomyces cerevisiae

(2019) Muhammad Saad Ahmed et al.   BIOCHEMICAL ENGINEERING JOURNAL

Recent developments in terminator technology in Saccharomyces cerevisiae

(2019) Takashi Matsuyama   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Molecular tools for pathway engineering in Saccharomyces cerevisiae

(2018) Pamela B Besada-Lombana et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA

(2018) Yasuyuki Nakamura et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli

(2018) Guillaume Cambray et al.   NATURE BIOTECHNOLOGY

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

Yeast Terminator Function Can Be Modulated and Designed on the Basis of Predictions of Nucleosome Occupancy

(2017) Nicholas J. Morse et al.   ACS Synthetic Biology

Construction, characterization and application of a genome-wide promoter library in Saccharomyces cerevisiae

(2017) Ting Yuan et al.   Frontiers of Chemical Science and Engineering

5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae

(2016) Hisashi Hoshida et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide

(2016) Kentaro Inokuma et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances

(2016) James M. Wagner et al.   FUNGAL GENETICS AND BIOLOGY

A GC-rich sequence feature in the 3′ UTR directs UPF1-dependent mRNA decay in mammalian cells

(2016) Naoto Imamachi et al.   GENOME RESEARCH

Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

(2016) Jan-Philipp Schwarzhans et al.   Microbial Cell Factories

Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae

(2016) Yoichiro Ito et al.   Scientific Reports

Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing

(2016) Jan-Philipp Schwarzhans et al.   Scientific Reports

An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

(2015) William C DeLoache et al.   Nature Chemical Biology

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast

(2015) Kathleen A. Curran et al.   ACS Synthetic Biology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

On the Normalization of the Minimum Free Energy of RNAs by Sequence Length

(2014) Edoardo Trotta   PLoS One

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae

(2013) Yoichiro Ito et al.   JOURNAL OF BIOTECHNOLOGY

Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications

(2013) Kathleen A. Curran et al.   METABOLIC ENGINEERING

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

A Genome-Wide Activity Assessment of Terminator Regions in Saccharomyces cerevisiae Provides a ″Terminatome″ Toolbox

(2013) Mamoru Yamanishi et al.   ACS Synthetic Biology

ViennaRNA Package 2.0

(2011) Ronny Lorenz et al.   Algorithms for Molecular Biology

Predicting nucleosome positioning using a duration Hidden Markov Model

(2010) Liqun Xi et al.   BMC BIOINFORMATICS

Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production

(2010) Gerhard Stadlmayr et al.   JOURNAL OF BIOTECHNOLOGY

A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell Labeling

(2009) Rita L. Strack et al.   BIOCHEMISTRY

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH

Transterm: a database to aid the analysis of regulatory sequences in mRNAs

(2008) G. H. Jacobs et al.   NUCLEIC ACIDS RESEARCH