State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila

Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes

(2021) Sarah E. Allen et al.   PLOS BIOLOGY

Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis inDrosophila

(2021) Gabriel T. Koreman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of Drosophila attP containing cell lines using CRISPR-Cas9

(2021) Daniel Mariyappa et al.   G3-Genes Genomes Genetics

Programmable RNA targeting with the single-protein CRISPR effector Cas7-11

(2021) Ahsen Özcan et al.   NATURE

Targeted gene disruption by CRISPR/xCas9 system in Drosophila melanogaster

(2020) Xu‐yang Ni et al.   ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

Large-Scale Transgenic Drosophila Resource Collections for Loss- and Gain-of-Function Studies

(2020) Jonathan Zirin et al.   GENETICS

A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila

(2020) Fillip Port et al.   eLife

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

(2020) Julian Grünewald et al.   NATURE BIOTECHNOLOGY

Base editors for simultaneous introduction of C-to-T and A-to-G mutations

(2020) Rina C. Sakata et al.   NATURE BIOTECHNOLOGY

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Glycosylase base editors enable C-to-A and C-to-G base changes

(2020) Dongdong Zhao et al.   NATURE BIOTECHNOLOGY

A programmable sequence of reporters for lineage analysis

(2020) Jorge Garcia-Marques et al.   NATURE NEUROSCIENCE

Programmable RNA Targeting Using CasRx in Flies

(2020) Anna B. Buchman et al.   CRISPR Journal

A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

(2020) Kevin G. Nyberg et al.   FLY

CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells

(2020) Baolong Xia et al.   Nature Protocols

Multiplexed conditional genome editing with Cas12a in Drosophila

(2020) Fillip Port et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precise genome engineering inDrosophilausing prime editing

(2020) Justin A. Bosch et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

(2019) Hagar Meltzer et al.   Nature Communications

Unlimited Genetic Switches for Cell-Type-Specific Manipulation

(2019) Jorge Garcia-Marques et al.   NEURON

Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids

(2019) Justin A. Bosch et al.   GENETICS

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

(2019) Oguz Kanca et al.   eLife

Gene activation by dCas9-CBP and the SAM system differ in target preference

(2019) Suresh Sajwan et al.   Scientific Reports

SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing

(2019) Chiao-Lin Chen et al.   G3-Genes Genomes Genetics

Advances in Engineering the Fly Genome with the CRISPR-Cas System

(2018) Ethan Bier et al.   GENETICS

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Next-generation CRISPR/Cas9 transcriptional activation inDrosophilausing flySAM

(2018) Yu Jia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ovo D Co-selection: A Method for Enriching CRISPR/Cas9-Edited Alleles in Drosophila

(2018) Ben Ewen-Campen et al.   G3-Genes Genomes Genetics

A gene-specific T2A-GAL4 library for Drosophila

(2018) Pei-Tseng Lee et al.   eLife

Advances in Engineering the Fly Genome with the CRISPR-Cas System

(2018) Ethan Bier et al.   GENETICS

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells

(2018) Raghuvir Viswanatha et al.   eLife

An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

(2018) David Li-Kroeger et al.   eLife

A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila

(2018) Naoki Okamoto et al.   DEVELOPMENTAL CELL

Target-Specific Precision of CRISPR-Mediated Genome Editing

(2018) Anob M. Chakrabarti et al.   MOLECULAR CELL

Generation of genome-modified Drosophila cell lines using SwAP

(2017) Alexandra Franz et al.   FLY

Nonhomologous DNA end-joining for repair of DNA double-strand breaks

(2017) Nicholas R. Pannunzio et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Use of the CRISPR-Cas9 system for genome editing in cultured Drosophila ovarian somatic cells

(2017) Hirotsugu Ishizu et al.   METHODS

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Optimized strategy for in vivo Cas9-activation inDrosophila

(2017) Ben Ewen-Campen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9

(2016) Abigail M. Lamb et al.   FLY

Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs

(2016) Fillip Port et al.   NATURE METHODS

Comparison of Cas9 activators in multiple species

(2016) Alejandro Chavez et al.   NATURE METHODS

Effective knockdown ofDrosophilalong non-coding RNAs by CRISPR interference

(2016) Sanjay Ghosh et al.   NUCLEIC ACIDS RESEARCH

CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

(2016) Jonathan L. Schmid-Burgk et al.   Nature Communications

Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion

(2016) D. T. Ge et al.   G3-Genes Genomes Genetics

Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy

(2016) Nanci S. Kane et al.   G3-Genes Genomes Genetics

A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells

(2016) Stefan Kunzelmann et al.   G3-Genes Genomes Genetics

Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology

(2015) Hideaki Mabashi-Asazuma et al.   ACS Chemical Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi

(2015) Benjamin E. Housden et al.   Science Signaling

Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research

(2015) Fillip Port et al.   G3-Genes Genomes Genetics

Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi

(2015) Benjamin E. Housden et al.   Science Signaling

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Alternative end-joining pathway(s): Bricolage at DNA breaks

(2014) Philippe Frit et al.   DNA REPAIR

Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair inDrosophila

(2014) Scott J. Gratz et al.   GENETICS

CRISPR/Cas9 mediated genome engineering in Drosophila

(2014) Andrew Bassett et al.   METHODS

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

(2014) F. Port et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Performance of the Cas9 Nickase System in Drosophila melanogaster

(2014) Xingjie Ren et al.   G3-Genes Genomes Genetics

CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila

(2014) Zhaoyu Xue et al.   G3-Genes Genomes Genetics

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila

(2013) Shu Kondo et al.   GENETICS

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Rewriting a genome

(2013) Emmanuelle Charpentier et al.   NATURE

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9

(2013) X. Ren et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9

(2013) A. R. Bassett et al.   Biology Open

In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration

(2012) Sandra Cristea et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy

(2012) Jiyong Liu et al.   Journal of Genetics and Genomics

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE