Fed-batch production assessment of a tetravalent bispecific antibody: A case study on piggyBac stably transfected HEK293 cells

A human expression system based on HEK293 for the stable production of recombinant erythropoietin

(2019) Christine Lin Chin et al.   Scientific Reports

Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin

(2019) Hubert Schwarz et al.   JOURNAL OF BIOTECHNOLOGY

Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1

(2019) Sarah Inwood et al.   New Biotechnology

Concomitant reduction of lactate and ammonia accumulation in fed-batch cultures: Impact on glycoprotein production and quality

(2018) Eric Karengera et al.   BIOTECHNOLOGY PROGRESS

Impact of Buffers on Colloidal Property and Aggregation Propensities of a Bispecific Antibody

(2018) Subhabrata Majumder et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Therapeutic glycoprotein production in mammalian cells

(2017) Marie-Eve Lalonde et al.   JOURNAL OF BIOTECHNOLOGY

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

(2016) Sowmya Balasubramanian et al.   BIOTECHNOLOGY PROGRESS

Generation of stable Chinese hamster ovary pools yielding antibody titers of up to 7.6 g/L using the piggyBac transposon system

(2016) Yashas Rajendra et al.   BIOTECHNOLOGY PROGRESS

Lactate and glucose concomitant consumption as a self-regulated pH detoxification mechanism in HEK293 cell cultures

(2015) Leticia Liste-Calleja et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

(2015) Sowmya Balasubramanian et al.   JOURNAL OF BIOTECHNOLOGY

Human cell lines for the production of recombinant proteins: on the horizon

(2015) Lukas Fliedl et al.   New Biotechnology

Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells

(2015) Sunandan Saha et al.   NUCLEIC ACIDS RESEARCH

Design of serum-free medium for suspension culture of CHO cells on the basis of general commercial media

(2014) Hideo Miki et al.   CYTOTECHNOLOGY

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

(2013) Z. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recent advances in technology supporting biopharmaceutical production from mammalian cells

(2012) M. Butler et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting

(2010) Johannes Pichler et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell death in mammalian cell culture: molecular mechanisms and cell line engineering strategies

(2010) Britta Krampe et al.   CYTOTECHNOLOGY

Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins

(2010) Darius Ghaderi et al.   NATURE BIOTECHNOLOGY

Expression systems for therapeutic glycoprotein production

(2009) Yves Durocher et al.   CURRENT OPINION IN BIOTECHNOLOGY

piggyBac can bypass DNA synthesis during cut and paste transposition

(2008) Rupak Mitra et al.   EMBO JOURNAL