Concomitant reduction of lactate and ammonia accumulation in fed-batch cultures: Impact on glycoprotein production and quality

Altering the central carbon metabolism of HEK293 cells: Impact on recombinant glycoprotein quality

(2017) Eric Karengera et al.   JOURNAL OF BIOTECHNOLOGY

Genetic defects in the hexosamine and sialic acid biosynthesis pathway

(2016) Anke P. Willems et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Metabolic engineering of CHO cells to alter lactate metabolism during fed-batch cultures

(2016) Cécile Toussaint et al.   JOURNAL OF BIOTECHNOLOGY

Limitations to the development of recombinant human embryonic kidney 293E cells using glutamine synthetase-mediated gene amplification: Methionine sulfoximine resistance

(2016) Da Young Yu et al.   JOURNAL OF BIOTECHNOLOGY

Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation

(2015) Bojiao Yin et al.   BIOTECHNOLOGY AND BIOENGINEERING

Chinese hamster ovary (CHO) host cell engineering to increase sialylation of recombinant therapeutic proteins by modulating sialyltransferase expression

(2015) Nan Lin et al.   BIOTECHNOLOGY PROGRESS

Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives

(2015) Jennifer Dumont et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Pyruvate carboxylase is critical for non–small-cell lung cancer proliferation

(2015) Katherine Sellers et al.   JOURNAL OF CLINICAL INVESTIGATION

Glutamine synthetase activity fuels nucleotide biosynthesis and supports growth of glutamine-restricted glioblastoma

(2015) Saverio Tardito et al.   NATURE CELL BIOLOGY

Human cell lines for the production of recombinant proteins: on the horizon

(2015) Lukas Fliedl et al.   New Biotechnology

Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody

(2014) Hengameh Aghamohseni et al.   JOURNAL OF BIOTECHNOLOGY

Effect of glutamine substitution by TCA cycle intermediates on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture

(2014) Tae Kwang Ha et al.   JOURNAL OF BIOTECHNOLOGY

Glutamine deprivation stimulates mTOR-JNK-dependent chemokine secretion

(2014) Naval P. Shanware et al.   Nature Communications

Exploiting the metabolism of PYC expressing HEK293 cells in fed-batch cultures

(2013) Cédric Vallée et al.   JOURNAL OF BIOTECHNOLOGY

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

(2013) Bo Liu et al.   JOURNAL OF BIOTECHNOLOGY

Fed-batch CHO cell t-PA production and feed glutamine replacement to reduce ammonia production

(2012) Do Yun Kim et al.   BIOTECHNOLOGY PROGRESS

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

(2012) Francesca Zagari et al.   New Biotechnology

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Inhibition of glutamine-dependent autophagy increases t-PA production in CHO Cell fed-batch processes

(2011) Mario A. Jardon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

(2011) Yashas Rajendra et al.   BIOTECHNOLOGY LETTERS

Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

(2011) Susan C Burleigh et al.   BMC BIOTECHNOLOGY

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

(2011) Meixia Zhou et al.   JOURNAL OF BIOTECHNOLOGY

Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth

(2011) Michael Taschwer et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase

(2011) Olivier Henry et al.   METABOLIC ENGINEERING

Pyruvate carboxylase is required for glutamine-independent growth of tumor cells

(2011) T. Cheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures

(2010) Danny Chee Furng Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

CHO-K1 host cells adapted to growth in glutamine-free medium by FACS-assisted evolution

(2010) Juan A. Hernández Bort et al.   Biotechnology Journal

The hexosamine biosynthetic pathway couples growth factor-induced glutamine uptake to glucose metabolism

(2010) K. E. Wellen et al.   GENES & DEVELOPMENT

Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins

(2010) Darius Ghaderi et al.   NATURE BIOTECHNOLOGY

Optimal and consistent protein glycosylation in mammalian cell culture

(2009) P. Hossler et al.   GLYCOBIOLOGY

Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

(2008) Martin Loignon et al.   BMC BIOTECHNOLOGY