Generation of stable Chinese hamster ovary pools yielding antibody titers of up to 7.6 g/L using the piggyBac transposon system

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines

(2015) Sowmya Balasubramanian et al.   BIOTECHNOLOGY AND BIOENGINEERING

Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY LETTERS

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

(2015) Sowmya Balasubramanian et al.   JOURNAL OF BIOTECHNOLOGY

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach

(2013) Jean-René Alattia et al.   BIOTECHNOLOGY AND BIOENGINEERING

A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression

(2013) Katharine Cain et al.   BIOTECHNOLOGY PROGRESS

A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

(2013) Olalekan Daramola et al.   BIOTECHNOLOGY PROGRESS

Development of a highly-efficient CHO cell line generation system with engineered SV40E promoter

(2013) Lianchun Fan et al.   JOURNAL OF BIOTECHNOLOGY

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

(2013) Z. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

(2011) Mattia Matasci et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells

(2011) Lianchun Fan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome-wide target profiling of piggyBac and Tol2 in HEK 293: pros and cons for gene discovery and gene therapy

(2011) Yaa-Jyuhn J Meir et al.   BMC BIOTECHNOLOGY

High-level transgene expression by homologous recombination-mediated gene transfer

(2011) Mélanie Grandjean et al.   NUCLEIC ACIDS RESEARCH

Rapid protein production using CHO stable transfection pools

(2010) Jianxin Ye et al.   BIOTECHNOLOGY PROGRESS

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs