Genetically encoded sensors towards imaging cAMP and PKA activity in vivo

Signaling Microdomains in the Spotlight: Visualizing Compartmentalized Signaling Using Genetically Encoded Fluorescent Biosensors

(2021) Jin-Fan Zhang et al.   Annual Review of Pharmacology and Toxicology

A versatile GPCR toolkit to track in vivo neuromodulation: not a one-size-fits-all sensor

(2021) Marie A. Labouesse et al.   NEUROPSYCHOPHARMACOLOGY

Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools

(2020) Tetsuya Watabe et al.   ACS Sensors

A rationally enhanced red fluorescent protein expands the utility of FRET biosensors

(2020) Gary C. H. Mo et al.   Nature Communications

An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice

(2020) Jin-Fan Zhang et al.   Nature Chemical Biology

Imaging Neurotransmitter and Neuromodulator Dynamics In Vivo with Genetically Encoded Indicators

(2020) Bernardo L. Sabatini et al.   NEURON

Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors

(2019) Hsien-Wei Yeh et al.   Annual Review of Analytical Chemistry

Therapeutic targeting of 3′,5′-cyclic nucleotide phosphodiesterases: inhibition and beyond

(2019) George S. Baillie et al.   NATURE REVIEWS DRUG DISCOVERY

Dual-Mode FRET and BRET Sensors for Detecting cAMP Dynamics

(2019) Alexander R. French et al.   ACS Omega

Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

(2018) Daria M. Shcherbakova et al.   Nature Chemical Biology

Red fluorescent cAMP indicator with increased affinity and expanded dynamic range

(2018) Yusaku Ohta et al.   Scientific Reports

A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging

(2018) Naoki Komatsu et al.   Scientific Reports

Interrogating the Spatiotemporal Landscape of Neuromodulatory GPCR Signaling by Real-Time Imaging of cAMP in Intact Neurons and Circuits

(2018) Brian S. Muntean et al.   Cell Reports

Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities

(2018) Sohum Mehta et al.   NATURE CELL BIOLOGY

A Highly Sensitive A-Kinase Activity Reporter for Imaging Neuromodulatory Events in Awake Mice

(2018) Lei Ma et al.   NEURON

Visualizing and discovering cellular structures with super-resolution microscopy

(2018) Yaron M. Sigal et al.   SCIENCE

An Inconvenient Truth: Calcium Sensors Are Calcium Buffers

(2018) Shane M. McMahon et al.   TRENDS IN NEUROSCIENCES

Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution

(2017) Gary C H Mo et al.   NATURE METHODS

Endogenous Gαq-Coupled Neuromodulator Receptors Activate Protein Kinase A

(2017) Yao Chen et al.   NEURON

Imaging ERK and PKA Activation in Single Dendritic Spines during Structural Plasticity

(2017) Shen Tang et al.   NEURON

FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility

(2017) Nicoletta C. Surdo et al.   Nature Communications

Activation of PKA in cell requires higher concentration of cAMP than in vitro: implications for compartmentalization of cAMP signalling

(2017) Andreas Koschinski et al.   Scientific Reports

Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM

(2017) Claire Demeautis et al.   Scientific Reports

Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates

(2017) Shane E. Tillo et al.   Cell Reports

Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging

(2017) Kazuki Harada et al.   Scientific Reports

Nontrivial Effect of the Color-Exchange of a Donor/Acceptor Pair in the Engineering of Förster Resonance Energy Transfer (FRET)-Based Indicators

(2016) Yusaku Ohta et al.   ACS Chemical Biology

A novel biosensor to study cAMP dynamics in cilia and flagella

(2016) Shatanik Mukherjee et al.   eLife

The cAMP Pathway as Therapeutic Target in Autoimmune and Inflammatory Diseases

(2016) Verena Katharina Raker et al.   Frontiers in Immunology

A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy

(2015) Wen Tao et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Quantitative two-photon imaging of fluorescent biosensors

(2015) Gary Yellen et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus

(2015) H. B. Fernandes et al.   JOURNAL OF NEUROSCIENCE

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

(2015) Yidan Ding et al.   NATURE METHODS

Applying superresolution localization-based microscopy to neurons

(2015) Haining Zhong   SYNAPSE

Involvement of cAMP-guanine nucleotide exchange factor II in hippocampal long-term depression and behavioral flexibility

(2015) Kyungmin Lee et al.   Molecular Brain

Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

(2015) Jeffrey Klarenbeek et al.   PLoS One

Decoding spatial and temporal features of neuronal cAMP/PKA signaling with FRET biosensors

(2014) Liliana R. V. Castro et al.   Biotechnology Journal

High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells

(2014) Sergi Regot et al.   CELL

Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems

(2014) V. Sample et al.   JOURNAL OF CELL SCIENCE

A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging

(2014) Yao Chen et al.   Frontiers in Pharmacology

Single-fluorophore biosensors based on conformation-sensitive GFP variants

(2013) Agnès Bonnot et al.   FASEB JOURNAL

CRIS—A Novel cAMP-Binding Protein Controlling Spermiogenesis and the Development of Flagellar Bending

(2013) Anke Miriam Krähling et al.   PLoS Genetics

Social, Communication, and Cortical Structural Impairments in Epac2-Deficient Mice

(2012) D. P. Srivastava et al.   JOURNAL OF NEUROSCIENCE

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

EPAC Null Mutation Impairs Learning and Social Interactions via Aberrant Regulation of miR-124 and Zif268 Translation

(2012) Ying Yang et al.   NEURON

Dopaminergic Modulation of Synaptic Transmission in Cortex and Striatum

(2012) Nicolas X. Tritsch et al.   NEURON

Structural Analyses of a Constitutively Active Mutant of Exchange Protein Directly Activated by cAMP

(2012) Mark A. White et al.   PLoS One

A Luminescent Biosensor with Increased Dynamic Range for Intracellular cAMP

(2011) Brock F. Binkowski et al.   ACS Chemical Biology

An Ion-insensitive cAMP Biosensor for Long Term Quantitative Ratiometric Fluorescence Resonance Energy Transfer (FRET) Measurements under Variable Physiological Conditions

(2011) Petrus S. Salonikidis et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Luminescent Kinase Activity Biosensors Based on a Versatile Bimolecular Switch

(2011) Katie J. Herbst et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range

(2011) Jeffrey B. Klarenbeek et al.   PLoS One

Type 4 Phosphodiesterase Plays Different Integrating Roles in Different Cellular Domains in Pyramidal Cortical Neurons

(2010) L. R. V. Castro et al.   JOURNAL OF NEUROSCIENCE

Visualization of PKA activity in plasma membrane microdomains

(2010) Charlene Depry et al.   Molecular BioSystems

Signaling diversity of PKA achieved via a Ca2+-cAMP-PKA oscillatory circuit

(2010) Qiang Ni et al.   Nature Chemical Biology

Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte

(2009) R. P. Norris et al.   DEVELOPMENT

The role of membrane microdomains in shaping β2-adrenergic receptor-mediated cAMP dynamics

(2009) Lisa M. DiPilato et al.   Molecular BioSystems

Capturing adenylyl cyclases as potential drug targets

(2009) Sandra Pierre et al.   NATURE REVIEWS DRUG DISCOVERY

Subcellular Dynamics of Type II PKA in Neurons

(2009) Haining Zhong et al.   NEURON

Cell Signaling in Space and Time: Where Proteins Come Together and When They're Apart

(2009) J. D. Scott et al.   SCIENCE

Persistent cAMP-Signals Triggered by Internalized G-Protein–Coupled Receptors

(2009) Davide Calebiro et al.   PLOS BIOLOGY

Novel Genetically Encoded Biosensors Using Firefly Luciferase

(2008) Frank Fan et al.   ACS Chemical Biology

Imaging second messenger dynamics in developing neural circuits

(2008) Timothy A. Dunn et al.   Developmental Neurobiology

Cyclic Nucleotide-regulated Cation Channels

(2008) Martin Biel   JOURNAL OF BIOLOGICAL CHEMISTRY

Cortical Adenylyl Cyclase 1 Is Required for Thalamocortical Synapse Maturation and Aspects of Layer IV Barrel Development

(2008) T. Iwasato et al.   JOURNAL OF NEUROSCIENCE

Activation of exchange protein activated by cyclic-AMP enhances long-lasting synaptic potentiation in the hippocampus

(2008) J. N. Gelinas et al.   LEARNING & MEMORY

Pharmacological Characterization of Membrane-Expressed Human Trace Amine-Associated Receptor 1 (TAAR1) by a Bioluminescence Resonance Energy Transfer cAMP Biosensor

(2008) L. S. Barak et al.   MOLECULAR PHARMACOLOGY

Widespread Receptivity to Neuropeptide PDF throughout the Neuronal Circadian Clock Network of Drosophila Revealed by Real-Time Cyclic AMP Imaging

(2008) Orie T. Shafer et al.   NEURON

The cyclic AMP phenotype of fragile X and autism

(2008) D KELLEY et al.   NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS

A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

(2008) Gerard N. M. van der Krogt et al.   PLoS One

A Tunable FRET Circuit for Engineering Fluorescent Biosensors

(2007) Michael D. Allen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION