Separation of charge variants of a monoclonal antibody by overloaded ion exchange chromatography

A case study of the mechanism of unfolding and aggregation of a monoclonal antibody in ion exchange chromatography

(2020) Izabela Poplewska et al.   JOURNAL OF CHROMATOGRAPHY A

A high-throughput method for fast detecting unfolding of monoclonal antibodies on cation exchange resins

(2020) Artur Stańczak et al.   JOURNAL OF CHROMATOGRAPHY A

Selectivity enhanced cation exchange chromatography for simultaneous determination of peptide variants

(2019) Sameh Ahmed et al.   TALANTA

Separation of monoclonal antibody charge variants using cation exchange chromatography: Resins and separation conditions optimization

(2019) Shu-Ying Jing et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Industrial bioprocessing perspectives on managing therapeutic protein charge variant profiles

(2018) Stanley Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Evaluation of differences between dual salt-pH gradient elution and mono gradient elution using a thermodynamic model: Simultaneous separation of six monoclonal antibody charge and size variants on preparative-scale ion exchange chromatographic resin

(2018) Yi Feng Lee et al.   BIOTECHNOLOGY PROGRESS

Design of two-column batch-to-batch recirculation to enhance performance in ion-exchange chromatography

(2018) Oliver Persson et al.   JOURNAL OF CHROMATOGRAPHY A

Prediction tool for loading, isocratic elution, gradient elution and scaling up of ion exchange chromatography of proteins

(2018) Wojciech Kazimierz Marek et al.   JOURNAL OF CHROMATOGRAPHY A

Multi-column displacement chromatography for separation of charge variants of monoclonal antibodies

(2018) Ohnmar Khanal et al.   JOURNAL OF CHROMATOGRAPHY A

Microheterogeneity of therapeutic monoclonal antibodies is governed by changes in the surface charge of the protein

(2016) Beate Hintersteiner et al.   Biotechnology Journal

Evaluation of Heavy-Chain C-Terminal Deletion on Product Quality and Pharmacokinetics of Monoclonal Antibodies

(2016) Guoying Jiang et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Charge heterogeneity: Basic antibody charge variants with increased binding to Fc receptors

(2016) Beate Hintersteiner et al.   mAbs

Microheterogeneity of therapeutic monoclonal antibodies is governed by changes in the surface charge of the protein

(2016) Beate Hintersteiner et al.   Biotechnology Journal

Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, Part I: Salt gradient approach

(2015) Szabolcs Fekete et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, Part II: pH gradient approach

(2015) Szabolcs Fekete et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Unfolding of a model protein on ion exchange and mixed mode chromatography surfaces

(2014) Adrian M. Gospodarek et al.   JOURNAL OF CHROMATOGRAPHY A

Unfolding and aggregation of a glycosylated monoclonal antibody on a cation exchange column. Part II. Protein structure effects by hydrogen deuterium exchange mass spectrometry

(2014) Jing Guo et al.   JOURNAL OF CHROMATOGRAPHY A

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Near-infrared and two-dimensional fluorescence spectroscopy monitoring of monoclonal antibody fermentation media quality: Aged media decreases cell growth

(2013) Christian Hakemeyer et al.   Biotechnology Journal

Mid-infrared spectroscopy-based antibody aggregate quantification in cell culture fluids

(2013) Florian Capito et al.   Biotechnology Journal

Systematic generation of buffer systems for pH gradient ion exchange chromatography and their application

(2013) Frieder Kröner et al.   JOURNAL OF CHROMATOGRAPHY A

Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. II: Dynamics of the integrated separation on ion exchange and hydrophobic interaction chromatography media

(2013) Wojciech Marek et al.   JOURNAL OF CHROMATOGRAPHY A

Highly linear pH gradients for analyzing monoclonal antibody charge heterogeneity in the alkaline range

(2013) Nico Lingg et al.   JOURNAL OF CHROMATOGRAPHY A

Modeling of salt and pH gradient elution in ion-exchange chromatography

(2013) Michael Schmidt et al.   JOURNAL OF SEPARATION SCIENCE

Biosimilar, Biobetter, and Next Generation Antibody Characterization by Mass Spectrometry

(2012) Alain Beck et al.   ANALYTICAL CHEMISTRY

The sweet tooth of biopharmaceuticals: Importance of recombinant protein glycosylation analysis

(2012) Nico Lingg et al.   Biotechnology Journal

Therapeutic application of monoclonal antibodies in cancer: advances and challenges

(2012) H. Modjtahedi et al.   BRITISH MEDICAL BULLETIN

Cation exchange surface-mediated denaturation of an aglycosylated immunoglobulin (IgG1)

(2012) Ron Gillespie et al.   JOURNAL OF CHROMATOGRAPHY A

Improving pH gradient cation-exchange chromatography of monoclonal antibodies by controlling ionic strength

(2012) Liangyi Zhang et al.   JOURNAL OF CHROMATOGRAPHY A

Understanding protein adsorption phenomena at solid surfaces

(2011) Michael Rabe et al.   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Isolation and characterization of therapeutic antibody charge variants using cation exchange displacement chromatography

(2011) Taylor Zhang et al.   JOURNAL OF CHROMATOGRAPHY A

What's fueling the biotech engine—2010 to 2011

(2011) Saurabh Aggarwal   NATURE BIOTECHNOLOGY

Effects of Charge on Antibody Tissue Distribution and Pharmacokinetics

(2010) C. Andrew Boswell et al.   BIOCONJUGATE CHEMISTRY

Separation of oxidized variants of a monoclonal antibody by anion-exchange

(2010) Glen Teshima et al.   JOURNAL OF CHROMATOGRAPHY A

Charge variants in IgG1

(2010) Leslie A. Khawli et al.   mAbs

Reduced elimination of IgG antibodies by engineering the variable region

(2010) T. Igawa et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Recent advances in large-scale production of monoclonal antibodies and related proteins

(2010) Abhinav A. Shukla et al.   TRENDS IN BIOTECHNOLOGY

Multiproduct High-Resolution Monoclonal Antibody Charge Variant Separations by pH Gradient Ion-Exchange Chromatography

(2009) Dell Farnan et al.   ANALYTICAL CHEMISTRY

Effects of thermal heterogeneity in hydrophobic interaction chromatography

(2009) Renata Muca et al.   JOURNAL OF CHROMATOGRAPHY A

Quantitative analysis of monoclonal antibodies by cation-exchange chromatofocusing

(2009) Anna Rozhkova   JOURNAL OF CHROMATOGRAPHY A

Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP)

(2008) Thomas Müller-Späth et al.   BIOTECHNOLOGY AND BIOENGINEERING

Separation of protein charge variants with induced pH gradients using anion exchange chromatographic columns

(2008) Timothy M. Pabst et al.   BIOTECHNOLOGY PROGRESS

Protein separations with induced pH gradients using cation-exchange chromatographic columns containing weak acid groups

(2008) Timothy M. Pabst et al.   JOURNAL OF CHROMATOGRAPHY A

Heterogeneity of Monoclonal Antibodies

(2007) Hongcheng Liu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES