In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in Escherichia coli (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their Components

First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts

(2021) Sapna Badhan et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Necessity for Validation of Effectiveness of Selected Guide RNA In Silico for Application of CRISPR/Cas9

(2021) Dong-Hwan Kim et al.   MOLECULAR BIOTECHNOLOGY

Precision genome editing using cytosine and adenine base editors in mammalian cells

(2021) Tony P. Huang et al.   Nature Protocols

CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew

(2021) Dibyajyoti Pramanik et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

High-efficiency and multiplex adenine base editing in plants using new TadA variants

(2021) Daqi Yan et al.   Molecular Plant

Detect-seq reveals out-of-protospacer editing and target-strand editing by cytosine base editors

(2021) Zhixin Lei et al.   NATURE METHODS

Development of a base editor for protein evolution via in situ mutation in vivo

(2021) Wenliang Hao et al.   NUCLEIC ACIDS RESEARCH

A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing

(2021) Yaojun Tong et al.   Nature Communications

Cytosine Base Editor-Mediated Multiplex Genome Editing to Accelerate Discovery of Novel Antibiotics in Bacillus subtilis and Paenibacillus polymyxa

(2021) Man Su Kim et al.   Frontiers in Microbiology

Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

(2021) Arik Shams et al.   Nature Communications

GO: a functional reporter system to identify and enrich base editing activity

(2020) Alyna Katti et al.   NUCLEIC ACIDS RESEARCH

Are the current gRNA ranking prediction algorithms useful for genome editing in plants?

(2020) Fatima Naim et al.   PLoS One

Efficient genome editing of rubber tree (hevea brasiliensis) protoplasts using CRISPR/Cas9 ribonucleoproteins

(2020) Yueting Fan et al.   INDUSTRIAL CROPS AND PRODUCTS

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

A multiplex guide RNA expression system and its efficacy for plant genome engineering

(2020) Youngbin Oh et al.   Plant Methods

Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning

(2020) Mandana Arbab et al.   CELL

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Development of multiplex genome editing toolkits for citrus with high efficacy in biallelic and homozygous mutations

(2020) Xiaoen Huang et al.   PLANT MOLECULAR BIOLOGY

DNA capture by a CRISPR-Cas9–guided adenine base editor

(2020) Audrone Lapinaite et al.   SCIENCE

CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis

(2020) Sili Yu et al.   ACS Synthetic Biology

Development and Application of CRISPR/Cas in Microbial Biotechnology

(2020) Wentao Ding et al.   Frontiers in Bioengineering and Biotechnology

CRISPR-Assisted Multiplex Base Editing System in Pseudomonas putida KT2440

(2020) Jun Sun et al.   Frontiers in Bioengineering and Biotechnology

Tomato protoplasts as cell target for ribonucleoprotein (RNP)-mediated multiplexed genome editing

(2020) Alessandro Nicolia et al.   PLANT CELL TISSUE AND ORGAN CULTURE

CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

(2020) Dibyajyoti Pramanik et al.   Molecular Plant

Efficient CRISPR-mediated base editing inAgrobacteriumspp.

(2020) Savio D. Rodrigues et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii

(2019) Qi Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum

(2019) Yu Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome Editing in Agriculture: Technical and Practical Considerations

(2019) Julia Jansing et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Continuous evolution of base editors with expanded target compatibility and improved activity

(2019) Benjamin W. Thuronyi et al.   NATURE BIOTECHNOLOGY

Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST

(2019) Yaojun Tong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expanding C–T base editing toolkit with diversified cytidine deaminases

(2019) Tian-Lin Cheng et al.   Nature Communications

Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

(2019) Yawei Zhao et al.   Science China-Life Sciences

MACBETH: Multiplex automated Corynebacterium glutamicum base editing method

(2018) Yu Wang et al.   METABOLIC ENGINEERING

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

(2018) Ryan Marshall et al.   MOLECULAR CELL

Modeling the Embrace of a Mutator: APOBEC Selection of Nucleic Acid Ligands

(2018) Jason D. Salter et al.   TRENDS IN BIOCHEMICAL SCIENCES

Deaminase-mediated multiplex genome editing in Escherichia coli

(2018) Satomi Banno et al.   Nature Microbiology

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

(2018) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana

(2017) Florian Hahn et al.   Frontiers in Plant Science

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

The APOBEC Protein Family: United by Structure, Divergent in Function

(2016) Jason D. Salter et al.   TRENDS IN BIOCHEMICAL SCIENCES

Internal guide RNA interactions interfere with Cas9-mediated cleavage

(2016) Summer B. Thyme et al.   Nature Communications

EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology

(2016) Simon J. Moore et al.   ACS Synthetic Biology

High-throughput recombinant protein expression inEscherichia coli: current status and future perspectives

(2016) Baolei Jia et al.   Open Biology

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

(2016) R Stefan Isaac et al.   eLife

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

(2015) Michael E. Lee et al.   ACS Synthetic Biology

A Golden Gate Modular Cloning Toolbox for Plants

(2014) Carola Engler et al.   ACS Synthetic Biology

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One