CRISPR-Assisted Multiplex Base Editing System in Pseudomonas putida KT2440

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii

(2019) Qi Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Combination of ssDNA recombineering and CRISPR-Cas9 for Pseudomonas putida KT2440 genome editing

(2019) Zhixin Wu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum

(2019) Yu Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

(2018) Takayuki Arazoe et al.   Biotechnology Journal

MACBETH: Multiplex automated Corynebacterium glutamicum base editing method

(2018) Yu Wang et al.   METABOLIC ENGINEERING

Metabolic engineering of Pseudomonas taiwanensis VLB120 with minimal genomic modifications for high-yield phenol production

(2018) Benedikt Wynands et al.   METABOLIC ENGINEERING

Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

(2018) Jun Sun et al.   Microbial Cell Factories

Development of a Plasmid-Free Biosynthetic Pathway for Enhanced Muconic Acid Production in Pseudomonas chlororaphis HT66

(2018) Songwei Wang et al.   ACS Synthetic Biology

Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

(2018) Takayuki Arazoe et al.   Biotechnology Journal

Web-based design and analysis tools for CRISPR base editing

(2018) Gue-Ho Hwang et al.   BMC BIOINFORMATICS

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

Construction and Characterization of Broad-Spectrum Promoters for Synthetic Biology

(2017) Sen Yang et al.   ACS Synthetic Biology

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

(2017) Tomás Aparicio et al.   Biotechnology Journal

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions

(2016) Pablo I Nikel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

CasOT: a genome-wide Cas9/gRNA off-target searching tool

(2014) A. Xiao et al.   BIOINFORMATICS

Biotechnological domestication of pseudomonads using synthetic biology

(2014) Pablo I. Nikel et al.   NATURE REVIEWS MICROBIOLOGY

Features of pseudomonads growing at low temperatures: another facet of their versatility

(2014) Renata Moreno et al.   Environmental Microbiology Reports

Industrial biotechnology of Pseudomonas putida and related species

(2012) Ignacio Poblete-Castro et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of a Method for Markerless Gene Deletion in Pseudomonas putida

(2011) Nadja Graf et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

(2010) Rubing Liang et al.   BMC MICROBIOLOGY