Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos

(2019) Erwei Zuo et al.   SCIENCE

Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice

(2019) Shuai Jin et al.   SCIENCE

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii

(2019) Qi Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases

(2019) Daesik Kim et al.   Annual Review of Biochemistry

CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications

(2019) Kutubuddin A. Molla et al.   TRENDS IN BIOTECHNOLOGY

Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum

(2019) Yu Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPR base editors: genome editing without double-stranded breaks

(2018) Ayman Eid et al.   BIOCHEMICAL JOURNAL

Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

(2018) Takayuki Arazoe et al.   Biotechnology Journal

CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces

(2018) Yawei Zhao et al.   Biotechnology Journal

MACBETH: Multiplex automated Corynebacterium glutamicum base editing method

(2018) Yu Wang et al.   METABOLIC ENGINEERING

Highly efficient base editing in Staphylococcus aureus using an engineered CRISPR RNA-guided cytidine deaminase

(2018) Tongnian Gu et al.   Chemical Science

Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

(2018) Takayuki Arazoe et al.   Biotechnology Journal

CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces

(2018) Yawei Zhao et al.   Biotechnology Journal

Deaminase-mediated multiplex genome editing in Escherichia coli

(2018) Satomi Banno et al.   Nature Microbiology

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes

(2017) Gaelen T. Hess et al.   MOLECULAR CELL

CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons

(2017) Pierre Billon et al.   MOLECULAR CELL

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

(2017) Zenpei Shimatani et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

(2017) Mingzi M Zhang et al.   Nature Chemical Biology

CRISPR/Cas9-mediated correction of human genetic disease

(2017) Ke Men et al.   Science China-Life Sciences

CRISPR/Cas9 system: a powerful technology for in vivo and ex vivo gene therapy

(2017) Xiaohui Zhang et al.   Science China-Life Sciences

A decade of discovery: CRISPR functions and applications

(2017) Rodolphe Barrangou et al.   Nature Microbiology

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

An overview of rapamycin: from discovery to future perspectives

(2016) Young Ji Yoo et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Gifted microbes for genome mining and natural product discovery

(2016) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Characterization of negative regulatory genes for the biosynthesis of rapamycin in Streptomyces rapamycinicus and its application for improved production

(2014) Young Ji Yoo et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Thoughts and facts about antibiotics: Where we are now and where we are heading

(2012) János Bérdy   JOURNAL OF ANTIBIOTICS

DNA Repair in Mammalian Cells

(2009) C. Kunz et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS