Coimmobilization of lipases exhibiting three very different stability ranges. Reuse of the active enzymes and selective discarding of the inactivated ones

Cry3Aa*SpyCatcher Fusion Crystals Produced in Bacteria as Scaffolds for Multienzyme Coimmobilization

(2022) Qian Sun et al.   BIOCONJUGATE CHEMISTRY

Incorporation of Metals and Enzymes with Porous Imine Molecule Cages for Highly Efficient Semiheterogeneous Chemoenzymatic Catalysis

(2021) Shiqi Gao et al.   ACS Catalysis

Multi-enzyme co-immobilized nano-assemblies: Bringing enzymes together for expanding bio-catalysis scope to meet biotechnological challenges

(2021) Muhammad Bilal et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme

(2021) Roberto Morellon-Sterling et al.   ACS Sustainable Chemistry & Engineering

Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

(2021) Rafael C. Rodrigues et al.   BIOTECHNOLOGY ADVANCES

The combination of covalent and ionic exchange immobilizations enables the coimmobilization on vinyl sulfone activated supports and the reuse of the most stable immobilized enzyme

(2021) Sara Arana-Peña et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Enzyme-support interactions and inactivation conditions determine Thermomyces lanuginosus lipase inactivation pathways: Functional and florescence studies

(2021) Priscila M. Paiva Souza et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

(2021) Saadiah A. Abdulmalek et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts

(2021) Eleftheria Diamanti et al.   ACS Catalysis

Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions

(2021) Regine Siedentop et al.   Catalysts

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

(2020) Sara Arana-Peña et al.   Catalysts

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

(2020) Sara Arana-Peña et al.   BIOTECHNOLOGY ADVANCES

Multi‐enzyme Cascade Reactions in Metal‐Organic Frameworks

(2020) Jieying Liang et al.   CHEMICAL RECORD

Co-immobilization multienzyme nanoreactor with co-factor regeneration for conversion of CO2

(2020) Sizhu Ren et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Multi-Combilipases: Co-Immobilizing Lipases with Very Different Stabilities Combining Immobilization via Interfacial Activation and Ion Exchange. The Reuse of the Most Stable Co-Immobilized Enzymes after Inactivation of the Least Stable Ones

(2020) Sara Arana-Peña et al.   Catalysts

Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

(2020) MengYa Guo et al.   CATALYSIS COMMUNICATIONS

Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw

(2020) Xiaoxiao Yu et al.   BIORESOURCE TECHNOLOGY

Improvement of biodiesel production from palm oil by co-immobilization of Thermomyces lanuginosa lipase and Candida antarctica lipase B: Optimization using response surface methodology

(2020) Mansour Shahedi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions

(2019) Rafael C. Rodrigues et al.   BIOTECHNOLOGY ADVANCES

Multienzymatic Cascade Reactions via Enzyme Complex by Immobilization

(2019) Ee Taek Hwang et al.   ACS Catalysis

Reuse of Lipase from Pseudomonas fluorescens via Its Step-by-Step Coimmobilization on Glyoxyl-Octyl Agarose Beads with Least Stable Lipases

(2019) Rios et al.   Catalysts

New applications of glyoxyl-octyl agarose in lipases co-immobilization: Strategies to reuse the most stable lipase

(2019) Sara Arana-Peña et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ordered Coimmobilization of a Multienzyme Cascade System with a Metal Organic Framework in a Membrane: Reduction of CO2 to Methanol

(2019) Dailian Zhu et al.   ACS Applied Materials & Interfaces

Co-Immobilization of Tri-Enzymes for the Conversion of Hydroxymethylfurfural to 2,5-Diformylfuran

(2019) Zhuofu Wu et al.   MOLECULES

Immobilization of formate dehydrogenase on polyethylenimine‐grafted graphene oxide with kinetics and stability study

(2019) Peng Lin et al.   ENGINEERING IN LIFE SCIENCES

Sequential Co-immobilization of Enzymes in Metal-Organic Frameworks for Efficient Biocatalytic Conversion of Adsorbed CO2 to Formate

(2019) Yan Li et al.   Frontiers in Bioengineering and Biotechnology

Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

(2019) Sandra Alonso et al.   Nature Catalysis

Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions

(2018) Jakeline Kathiele Poppe et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Rational Engineering of Multiple Active Sites in an Ester Hydrolase

(2018) Gerard Santiago et al.   BIOCHEMISTRY

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reactor

(2018) Jakeline Kathiele Poppe et al.   BIOTECHNOLOGY PROGRESS

One-step Synthesis of α-Keto Acids from Racemic Amino Acids by A Versatile Immobilized Multienzyme Cell-free System

(2018) Alejandro H. Orrego et al.   ChemCatChem

Combi-metal organic framework (Combi-MOF) of α-amylase and glucoamylase for one pot starch hydrolysis

(2018) Manish Salgaonkar et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Improving the stability and reusability of dextranase by immobilization on polyethylenimine modified magnetic particles

(2018) Yajie Wang et al.   NEW JOURNAL OF CHEMISTRY

Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol

(2018) Khodabakhsh Rashidi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Optimization of a dual-functional biocatalytic system for continuous hydrolysis of lactose in milk

(2018) Heng Li et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Self-Sufficient Flow-Biocatalysis by Coimmobilization of Pyridoxal 5′-Phosphate and ω-Transaminases onto Porous Carriers

(2018) Ana I. Benítez-Mateos et al.   ACS Sustainable Chemistry & Engineering

Immobilization on octyl-agarose beads and some catalytic features of commercial preparations of lipase a from Candida antarctica (Novocor ADL): Comparison with immobilized lipase B from Candida antarctica

(2018) Sara Arana-Peña et al.   BIOTECHNOLOGY PROGRESS

Advances and opportunities for the design of self-sufficient and spatially organized cell-free biocatalytic systems

(2018) Claudia Schmid-Dannert et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

An improved amperometric triglyceride biosensor based on co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode

(2017) Vinay Narwal et al.   ENZYME AND MICROBIAL TECHNOLOGY

Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes

(2017) Heng Li et al.   MOLECULES

Physical crosslinking of lipase from Rhizomucor miehei immobilized on octyl agarose via coating with ionic polymers

(2017) Laura Fernandez-Lopez et al.   PROCESS BIOCHEMISTRY

Coimmobilization of enzymes in bilayers using pei as a glue to reuse the most stable enzyme: Preventing pei release during inactivated enzyme desorption

(2017) Hadjer Zaak et al.   PROCESS BIOCHEMISTRY

Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts

(2017) Jose J. Virgen-Ortíz et al.   Journal of Materials Chemistry B

Co-immobilized Phosphorylated Cofactors and Enzymes as Self-Sufficient Heterogeneous Biocatalysts for Chemical Processes

(2016) Susana Velasco-Lozano et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

(2016) Jerome Amoah et al.   BIORESOURCE TECHNOLOGY

Reversible immobilization of lipases on octyl-glutamic agarose beads: A mixed adsorption that reinforces enzyme immobilization

(2016) Nazzoly Rueda et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Effect of Molecular Structure on the Relative Hydrogen Peroxide Scavenging Ability of Some α-Keto Carboxylic Acids

(2016) Antonio Lopalco et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Co-immobilization and stabilization of xylanase, β-xylosidase and α-l-arabinofuranosidase from Penicillium janczewskii for arabinoxylan hydrolysis

(2016) César Rafael Fanchini Terrasan et al.   PROCESS BIOCHEMISTRY

Immobilized multienzymatic systems for catalysis of cascade reactions

(2016) Qingzhi Ji et al.   PROCESS BIOCHEMISTRY

Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment

(2016) Tiago L.de Albuquerque et al.   PROCESS BIOCHEMISTRY

Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase

(2016) Sara Peirce et al.   RSC Advances

Carrier-free co-immobilization of xylanase, cellulase and β-1,3-glucanase as combined cross-linked enzyme aggregates (combi-CLEAs) for one-pot saccharification of sugarcane bagasse

(2016) Karthik Periyasamy et al.   RSC Advances

Synthetic enzyme supercomplexes: co-immobilization of enzyme cascades

(2015) F. Kazenwadel et al.   Analytical Methods

Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

(2015) Evelin A. Manoel et al.   ENZYME AND MICROBIAL TECHNOLOGY

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment

(2015) Jose C.S. dos Santos et al.   ENZYME AND MICROBIAL TECHNOLOGY

Enhancing biodiesel production via a synergic effect between immobilized Rhizopus oryzae lipase and Novozym 435

(2015) Feng Su et al.   FUEL PROCESSING TECHNOLOGY

Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties

(2015) Jose C.S. dos Santos et al.   PROCESS BIOCHEMISTRY

Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization

(2015) Jose C. S. dos Santos et al.   RSC Advances

Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin

(2015) Jose C. S. dos Santos et al.   RSC Advances

Combining phospholipases and a liquid lipase for one-step biodiesel production using crude oils

(2014) Silvia Cesarini et al.   Biotechnology for Biofuels

Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts

(2014) Joana S. Alves et al.   RSC Advances

Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): A tri-enzyme biocatalyst with one pot starch hydrolytic activity

(2013) Sachin Talekar et al.   BIORESOURCE TECHNOLOGY

The combi-CLEA approach: enzymatic cascade synthesis of enantiomerically pure (S)-mandelic acid

(2013) Andrzej Chmura et al.   TETRAHEDRON-ASYMMETRY

Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison

(2012) Karel Hernandez et al.   CURRENT ORGANIC CHEMISTRY

Self-Assembly of Synthetic Metabolons through Synthetic Protein Scaffolds: One-Step Purification, Co-immobilization, and Substrate Channeling

(2012) Chun You et al.   ACS Synthetic Biology

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Multi-Enzymatic Cascade Reactions: Overview and Perspectives

(2011) Emanuele Ricca et al.   ADVANCED SYNTHESIS & CATALYSIS

Co-immobilization of cholesterol esterase, cholesterol oxidase and peroxidase on PVC strip for serum cholesterol determination

(2011) Nidhi Chauhan et al.   Analytical Methods

Co-immobilization of three cellulases on Au-doped magnetic silicananoparticles for the degradation of cellulose

(2011) Eun Jin Cho et al.   CHEMICAL COMMUNICATIONS

Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical process

(2010) Rafael C. Rodrigues et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium

(2010) Feifei Guan et al.   PROCESS BIOCHEMISTRY

Two conformational states of Candida rugosa lipase

(2010) Pawel Grochulski et al.   PROTEIN SCIENCE

Synthesis of Aliphatic (S)-α-Hydroxycarboxylic Amides using a One-Pot Bienzymatic Cascade of Immobilised Oxynitrilase and Nitrile Hydratase

(2009) Sander van Pelt et al.   ADVANCED SYNTHESIS & CATALYSIS

Transesterification for biodiesel production catalyzed by combined lipases: Optimization and kinetics

(2009) Yun Liu et al.   AICHE JOURNAL

Development of Batch and Continuous Processes on Biodiesel Production in a Packed-Bed Reactor by a Mixture of Immobilized Candida rugosa and Rhizopus oryzae Lipases

(2009) Jong Ho Lee et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Green and Efficient Conversion of CO2to Methanol by Biomimetic Coimmobilization of Three Dehydrogenases in Protamine-Templated Titania

(2009) Qianyun Sun et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Amperometric trienzyme ATP biosensors based on the coimmobilization of salicylate hydroxylase, glucose-6-phosphate dehydrogenase, and hexokinase

(2008) Yue Cui et al.   SENSORS AND ACTUATORS B-CHEMICAL

X-ray Structure of Candida antarctica Lipase A Shows a Novel Lid Structure and a Likely Mode of Interfacial Activation

(2007) Daniel J. Ericsson et al.   JOURNAL OF MOLECULAR BIOLOGY