Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme

Effect of Concentrated Salts Solutions on the Stability of Immobilized Enzymes: Influence of Inactivation Conditions and Immobilization Protocol

(2021) Sabrina Ait Braham et al.   MOLECULES

Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions

(2020) Jakub F. Kornecki et al.   PROCESS BIOCHEMISTRY

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

(2020) Sara Arana-Peña et al.   Catalysts

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

(2020) Sara Arana-Peña et al.   BIOTECHNOLOGY ADVANCES

Multi‐enzyme Cascade Reactions in Metal‐Organic Frameworks

(2020) Jieying Liang et al.   CHEMICAL RECORD

Coimmobilization and colocalization of a glycosyltransferase and a sucrose synthase greatly improves the recycling of UDP-glucose: Glycosylation of resveratrol 3-O-β-D-glucoside

(2020) Lara Trobo-Maseda et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

On the taught new tricks of enzymes immobilization: An all-inclusive overview

(2020) Roswanira Abdul Wahab et al.   REACTIVE & FUNCTIONAL POLYMERS

Dual-enzyme co-immobilization for the one-pot production of glucose 6-phosphate from maltodextrin

(2020) Jinxiu Zhang et al.   BIOCHEMICAL ENGINEERING JOURNAL

Multi-Combilipases: Co-Immobilizing Lipases with Very Different Stabilities Combining Immobilization via Interfacial Activation and Ion Exchange. The Reuse of the Most Stable Co-Immobilized Enzymes after Inactivation of the Least Stable Ones

(2020) Sara Arana-Peña et al.   Catalysts

Unlocking nature’s biosynthetic potential by directed genome evolution

(2020) Mingfeng Cao et al.   CURRENT OPINION IN BIOTECHNOLOGY

Co-immobilization of two-component hydroxylase monooxygenase by functionalized magnetic nanoparticles for preserving high catalytic activity and enhancing enzyme stabilty

(2020) Junya Liao et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw

(2020) Xiaoxiao Yu et al.   BIORESOURCE TECHNOLOGY

Directed evolution for enzyme development in biocatalysis

(2020) Serena Gargiulo et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Tailoring Multipurpose Biocatalysts via Protein Engineering Approaches: A Review

(2019) Muhammad Bilal et al.   CATALYSIS LETTERS

Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis

(2019) Roger A. Sheldon et al.   ChemSusChem

Reuse of Lipase from Pseudomonas fluorescens via Its Step-by-Step Coimmobilization on Glyoxyl-Octyl Agarose Beads with Least Stable Lipases

(2019) Rios et al.   Catalysts

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

(2019) Ge Qu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

New applications of glyoxyl-octyl agarose in lipases co-immobilization: Strategies to reuse the most stable lipase

(2019) Sara Arana-Peña et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Site-Specific and Covalent Immobilization of His-Tagged Proteins via Surface Vinyl Sulfone–Imidazole Coupling

(2019) Mingyang Li et al.   LANGMUIR

Parameters necessary to define an immobilized enzyme preparation

(2019) Joseph Boudrant et al.   PROCESS BIOCHEMISTRY

Pectin lyase immobilization using the glutaraldehyde chemistry increases the enzyme operation range

(2019) Lucas Dal Magro et al.   ENZYME AND MICROBIAL TECHNOLOGY

Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

(2019) Sandra Alonso et al.   Nature Catalysis

Rational Engineering of Multiple Active Sites in an Ester Hydrolase

(2018) Gerard Santiago et al.   BIOCHEMISTRY

One-step Synthesis of α-Keto Acids from Racemic Amino Acids by A Versatile Immobilized Multienzyme Cell-free System

(2018) Alejandro H. Orrego et al.   ChemCatChem

A new heterofunctional amino-vinyl sulfone support to immobilize enzymes: Application to the stabilization of β-galactosidase from A spergillus oryzae

(2018) Hadjer Zaak et al.   PROCESS BIOCHEMISTRY

Biomimetic enzyme cascade reaction system in microfluidic electrospray microcapsules

(2018) Huan Wang et al.   Science Advances

Optimization of a dual-functional biocatalytic system for continuous hydrolysis of lactose in milk

(2018) Heng Li et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Self-Sufficient Flow-Biocatalysis by Coimmobilization of Pyridoxal 5′-Phosphate and ω-Transaminases onto Porous Carriers

(2018) Ana I. Benítez-Mateos et al.   ACS Sustainable Chemistry & Engineering

Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes

(2017) Heng Li et al.   MOLECULES

Stabilization of ficin extract by immobilization on glyoxyl agarose. Preliminary characterization of the biocatalyst performance in hydrolysis of proteins

(2017) El-Hocine Siar et al.   PROCESS BIOCHEMISTRY

Coimmobilization of enzymes in bilayers using pei as a glue to reuse the most stable enzyme: Preventing pei release during inactivated enzyme desorption

(2017) Hadjer Zaak et al.   PROCESS BIOCHEMISTRY

Co-immobilized Phosphorylated Cofactors and Enzymes as Self-Sufficient Heterogeneous Biocatalysts for Chemical Processes

(2016) Susana Velasco-Lozano et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression

(2016) Jian-zhong Xu et al.   Journal of Zhejiang University-SCIENCE B

Ion exchange of β-galactosidase: The effect of the immobilization pH on enzyme stability

(2016) Tiago L. de Albuquerque et al.   PROCESS BIOCHEMISTRY

Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment

(2016) Tiago L.de Albuquerque et al.   PROCESS BIOCHEMISTRY

Vinyl sulfone-activated silica for efficient covalent immobilization of alkaline unstable enzymes: application to levansucrase for fructooligosaccharide synthesis

(2016) P. Santos-Moriano et al.   RSC Advances

Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase

(2016) Sara Peirce et al.   RSC Advances

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

(2015) Oveimar Barbosa et al.   BIOTECHNOLOGY ADVANCES

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment

(2015) Jose C.S. dos Santos et al.   ENZYME AND MICROBIAL TECHNOLOGY

Bovine trypsin immobilization on agarose activated with divinylsulfone: Improved activity and stability via multipoint covalent attachment

(2015) Jose C.S. dos Santos et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Immobilization of lipases on glyoxyl–octyl supports: Improved stability and reactivation strategies

(2015) Angélica Suescun et al.   PROCESS BIOCHEMISTRY

Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties

(2015) Jose C.S. dos Santos et al.   PROCESS BIOCHEMISTRY

Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization

(2015) Jose C. S. dos Santos et al.   RSC Advances

Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin

(2015) Jose C. S. dos Santos et al.   RSC Advances

Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

(2013) Juan C Begara-Morales et al.   BMC PLANT BIOLOGY

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

(2013) Jong Ho Lee et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Kinetic modeling of biodiesel production by mixed immobilized and co-immobilized lipase systems under two pressure conditions

(2013) Jong Ho Lee et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Enzymatic Hydrolysis of N-Benzoyl-l-Tyrosine p-Nitroanilide by α-Chymotrypsin in DMSO-Water/AOT/n-Heptane Reverse Micelles. A Unique Interfacial Effect on the Enzymatic Activity

(2013) Fernando Moyano et al.   LANGMUIR

6-Aminopenicillanic acid production in stationary basket bioreactor with packed bed of immobilized penicillin amidase—Penicillin G mass transfer and consumption rate under internal diffusion limitation

(2012) Dan Caşcaval et al.   BIOCHEMICAL ENGINEERING JOURNAL

Rational Co-Immobilization of Bi-Enzyme Cascades on Porous Supports and their Applications in Bio-Redox Reactions with In Situ Recycling of Soluble Cofactors

(2012) Javier Rocha-Martín et al.   ChemCatChem

Novel synthetic route for covalent coupling of biomolecules on super-paramagnetic hybrid nanoparticles

(2012) Antonio L. Medina-Castillo et al.   JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Multi-Enzymatic Cascade Reactions: Overview and Perspectives

(2011) Emanuele Ricca et al.   ADVANCED SYNTHESIS & CATALYSIS

Biodiesel production from crude canola oil by two-step enzymatic processes

(2011) Myung Gwi Jang et al.   RENEWABLE ENERGY

Vinyl sulfone functionalized silica: a “ready to use” pre-activated material for immobilization of biomolecules

(2010) Mariano Ortega-Muñoz et al.   JOURNAL OF MATERIALS CHEMISTRY

Purification, Characterization, and Solvent-Induced Thermal Stabilization of Ficin from Ficus carica

(2008) Kamsagara Basavarajappa Devaraj et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Metagenomics for Mining New Genetic Resources of Microbial Communities

(2008) Manuel Ferrer et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

Enzyme stability and stabilization—Aqueous and non-aqueous environment

(2008) Padma V. Iyer et al.   PROCESS BIOCHEMISTRY

Application and properties of butyl acrylate/pentaerythrite triacrylate copolymers and cellulose-based Granocel as carriers for trypsin immobilization

(2007) Jolanta Bryjak et al.   COLLOIDS AND SURFACES B-BIOINTERFACES