Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 186, Issue -, Pages 735-749Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.07.064
Keywords
Multienzymes; Co-immobilization; Nanocarriers; Nano-catalysis; Carbon materials; Polymeric cues; Silica; Metal particles
Funding
- Consejo Nacional de Ciencia y Tecnologia (CONACYT) [735340]
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Co-immobilization of multi-enzymes has emerged as a promising concept to design and signify bio-catalysis engineering, by combining various types of nanocarriers with different strategies to expand the scope of bio-catalysis.
Co-immobilization of multi-enzymes has emerged as a promising concept to design and signify bio-catalysis engineering. Undoubtedly, the existence and importance of basic immobilization methods such as encapsula-tion, covalent binding, cross-linking, or even simple adsorption cannot be ignored as they are the core of advanced co-immobilization strategies. Different strategies have been developed and deployed to green the twenty-first century bio-catalysis. Moreover, co-immobilization of multi-enzymes has successfully resolved the limitations of individual enzyme loaded constructs. With an added value of this advanced bio-catalysis engi-neering platform, designing, and fabricating co-immobilized enzymes loaded nanostructure carriers to perform a particular set of reactions with high catalytic turnover is of supreme interest. Herein, we spotlight the emergence of co-immobilization strategies by bringing multi-enzymes together with various types of nanocarriers to expand the bio-catalysis scope. Following a brief introduction, the first part of the review focuses on multienzyme co-immobilization strategies, i.e., random co-immobilization, compartmentalization, and positional co-immobilization. The second part comprehensively covers four major categories of nanocarriers, i.e., carbon based nanocarriers, polymer based nanocarriers, silica-based nanocarriers, and metal-based nanocarriers along with their particular examples. In each section, several critical factors that can affect the performance and successful deployment of co-immobilization of enzymes are given in this work.
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