Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

Rational Engineering of Multiple Active Sites in an Ester Hydrolase

(2018) Gerard Santiago et al.   BIOCHEMISTRY

Rational redesign of the active site of selenosubtilisin with strongly enhanced glutathione peroxidase activity

(2018) Tingting Wang et al.   JOURNAL OF CATALYSIS

A Rational Active-Site Redesign Converts a Decarboxylase into a C═C Hydratase: “Tethered Acetate” Supports Enantioselective Hydration of 4-Hydroxystyrenes

(2018) Stefan E. Payer et al.   ACS Catalysis

Engineered Biomolecular Catalysts

(2017) Kara L. Bren   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Adaptive simulations, towards interactive protein-ligand modeling

(2017) Daniel Lecina et al.   Scientific Reports

Directed evolution of artificial metalloenzymes for in vivo metathesis

(2016) Markus Jeschek et al.   NATURE

An artificial metalloenzyme with the kinetics of native enzymes

(2016) P. Dydio et al.   SCIENCE

Rational Enzyme Engineering Through Biophysical and Biochemical Modeling

(2016) Sandra Acebes et al.   ACS Catalysis

Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity

(2015) M. Filice et al.   CHEMICAL COMMUNICATIONS

Artificial metalloenzymes derived from three-helix bundles

(2015) Alison G Tebo et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

New Tricks for Old Proteins: Single Mutations in a Nonenzymatic Protein Give Rise to Various Enzymatic Activities

(2015) Yurii S. Moroz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Histidine Orientation Modulates the Structure and Dynamics of a de Novo Metalloenzyme Active Site

(2015) Matthew R. Ross et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

(2015) Ivana Drienovská et al.   Chemical Science

Short peptides self-assemble to produce catalytic amyloids

(2014) Caroline M. Rufo et al.   Nature Chemistry

De Novo Design of an Artificial Bis[4Fe-4S] Binding Protein

(2013) Anindya Roy et al.   BIOCHEMISTRY

Influence of Active Site Location on Catalytic Activity in de Novo-Designed Zinc Metalloenzymes

(2013) Melissa L. Zastrow et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Precision is essential for efficient catalysis in an evolved Kemp eliminase

(2013) Rebecca Blomberg et al.   NATURE

Elementary tetrahelical protein design for diverse oxidoreductase functions

(2013) Tammer A Farid et al.   Nature Chemical Biology

Artificial Metalloenzymes and Metallopeptide Catalysts for Organic Synthesis

(2013) Jared C. Lewis   ACS Catalysis

Enantioselective Artificial Metalloenzymes by Creation of a Novel Active Site at the Protein Dimer Interface

(2012) Jeffrey Bos et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Catalysis by a De Novo Zinc-Mediated Protein Interface: Implications for Natural Enzyme Evolution and Rational Enzyme Engineering

(2012) Bryan S. Der et al.   BIOCHEMISTRY

Computational Design of Catalytic Dyads and Oxyanion Holes for Ester Hydrolysis

(2012) Florian Richter et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis

(2012) Sagar D Khare et al.   Nature Chemical Biology

Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

(2012) Nobuhiko Tokuriki et al.   Nature Communications

Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

(2011) Olga Khersonsky et al.   JOURNAL OF MOLECULAR BIOLOGY

Hydrolytic catalysis and structural stabilization in a designed metalloprotein

(2011) Melissa L. Zastrow et al.   Nature Chemistry

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE