CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy

Pyruvate production by Escherichia coli using pyruvate dehydrogenase variants

(2021) W. Chris Moxley et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

dCas9 regulator to neutralize competition in CRISPRi circuits

(2021) Hsin-Ho Huang et al.   Nature Communications

Restoration of fitness lost due to dysregulation of the pyruvate dehydrogenase complex is triggered by ribosomal binding site modifications

(2021) Amitesh Anand et al.   Cell Reports

Gene Silencing Through CRISPR Interference in Bacteria: Current Advances and Future Prospects

(2021) Riyu Zhang et al.   Frontiers in Microbiology

Semi-rational design of L-amino acid deaminase for production of pyruvate and d-alanine by Escherichia coli whole-cell biocatalyst

(2021) Ke Liu et al.   AMINO ACIDS

Impact of CRISPR interference on strain development in biotechnology

(2020) Kerstin Schultenkämper et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Metabolic manipulation through CRISPRi and gene deletion to enhance cadaverine production in Escherichia coli

(2020) Wan-Wen Ting et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Strain engineering for high‐level 5‐aminolevulinic acid production in Escherichia coli

(2020) Dragan Miscevic et al.   BIOTECHNOLOGY AND BIOENGINEERING

Pyruvate-responsive genetic circuits for dynamic control of central metabolism

(2020) Xianhao Xu et al.   Nature Chemical Biology

Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale

(2020) Deepanwita Banerjee et al.   Nature Communications

Balancing glucose and oxygen uptake rates to enable high amorpha‐4,11‐diene production in Escherichia coli via the methylerythritol phosphate (MEP) pathway

(2020) Vikas Patil et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPR-based metabolic pathway engineering

(2020) Dongdong Zhao et al.   METABOLIC ENGINEERING

High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription

(2019) Scott A. Scholz et al.   Cell Systems

Improving isobutanol production with the yeast Saccharomyces cerevisiae by successively blocking competing metabolic pathways as well as ethanol and glycerol formation

(2019) Johannes Wess et al.   Biotechnology for Biofuels

CRISPRi-Based Downregulation of Transcriptional Feedback Improves Growth and Metabolism of Arginine Overproducing E. coli

(2019) Timur Sander et al.   ACS Synthetic Biology

Efficient biocatalyst of L-DOPA with Escherichia coli expressing a tyrosine phenol-lyase mutant from Kluyvera intermedia

(2019) Wei Yuan et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis

(2019) Yaokang Wu et al.   NUCLEIC ACIDS RESEARCH

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Gene repression via multiplex gRNA strategy in Y. lipolytica

(2018) Jin-lai Zhang et al.   Microbial Cell Factories

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

(2018) Lun Cui et al.   Nature Communications

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Identification and application of a growth-regulated promoter for improving l-valine production in Corynebacterium glutamicum

(2018) Yuechao Ma et al.   Microbial Cell Factories

Pyruvate dehydrogenase complex regulator (PdhR) gene deletion boosts glucose metabolism in Escherichia coli under oxygen-limited culture conditions

(2017) Soya Maeda et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Escherichia coli HGT: Engineered for high glucose throughput even under slowly growing or resting conditions

(2017) Annette Michalowski et al.   METABOLIC ENGINEERING

Metabolic assessment of E. coli as a Biofactory for commercial products

(2016) Xiaolin Zhang et al.   METABOLIC ENGINEERING

A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production

(2016) Tat-Ming Lo et al.   Cell Systems

Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites

(2015) Irene M. Brockman et al.   METABOLIC ENGINEERING

The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite

(2015) Luciano F. Huergo et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

A Vector Library for Silencing Central Carbon Metabolism Genes with Antisense RNAs in Escherichia coli

(2013) Nobutaka Nakashima et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

A novel membrane-integrated fermentation reactor system: application to pyruvic acid production in continuous culture by Torulopsis glabrata

(2011) Hideki Sawai et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Transcription Analysis of Central Metabolism Genes in Escherichia coli. Possible Roles of σ38 in Their Expression, as a Response to Carbon Limitation

(2009) Leticia Olvera et al.   PLoS One

High Glycolytic Flux Improves Pyruvate Production by a Metabolically Engineered Escherichia coli Strain

(2008) Y. Zhu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering BioBrick vectors from BioBrick parts

(2008) Reshma P Shetty et al.   Journal of Biological Engineering